Restriction map for
pF1KE0483
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# NO CUT ENZYMES ( 161 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AvaI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SwaI TaqI TfiI TthHB8I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AspI 484 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 926 GGACC G^G[AT]CC
BcnI 635 CCCGG CC^[GC]GG
BmyI 182 GTGCCC G[AGT]GC[ACT]^C
BsgI 646 GTGCAG GTGCAG(16/14)
BsmAI 628 GTCTC GTCTC(1/5)
Bsp1286I 182 GTGCCC G[AGT]GC[ACT]^C
Bsp1407I 1017 TGTACA T^GTACA
BsrGI 1017 TGTACA T^GTACA
BstEII 363 GGTCACC G^GT[ACGT]ACC
BstPI 363 GGTCACC G^GT[ACGT]ACC
DraII 925 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 925 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 363 GGTCACC G^GT[ACGT]ACC
FauI 667 GCGGG CCCGC(4/6)
HapII 636 CCGG C^CGG
HpaII 636 CCGG C^CGG
MspI 636 CCGG C^CGG
MwoI 349 GCAACAGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 635 CCCGG CC^[GC]GG
NspI 966 GCATGT [GA]CATG^[CT]
PflFI 484 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 925 GGGACCT [GA]G^G[AT]CC[CT]
SfaNI 371 GCATC GCATC(5/9)
SspBI 1017 TGTACA T^GTACA
SspI 120 AATATT AAT^ATT
Tth111I 484 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AccII 643 CGCG CG^CG
700 CGCG
AcsI 133 GAATTT [GA]^AATT[CT]
217 AAATTC
AfaI 383 GTAC GT^AC
1018 GTAC
AlwNI 353 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
494 CAGCTGCTG
ApoI 133 GAATTT [GA]^AATT[CT]
217 AAATTC
BglII 99 AGATCT A^GATCT
153 AGATCT
BpmI 263 CTGGAG CTGGAG(16/14)
832 CTGGAG
BsmFI 925 GGGAC GGGAC(10/14)
105 GTCCC
BspMI 928 ACCTGC ACCTGC(4/8)
648 GCAGGT
BstNI 9 CCAGG CC^[AT]GG
279 CCTGG
BstUI 643 CGCG CG^CG
700 CGCG
BstXI 818 CCAAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
899 CCAGAGGGGTGG
BstYI 99 AGATCT [GA]^GATC[CT]
153 AGATCT
Cfr13I 896 GGGCC G^G[ACGT]CC
926 GGACC
DdeI 520 CTTAG C^T[ACGT]AG
571 CTCAG
DpnII 100 GATC ^GATC
154 GATC
EarI 472 GAAGAG CTCTTC(1/4)
805 GAAGAG
EcoRII 9 CCAGG ^CC[AT]GG
279 CCTGG
HgaI 61 GACGC GACGC(5/10)
606 GCGTC
Ksp632I 472 GAAGAG CTCTTC(1/4)
805 GAAGAG
MaeIII 364 GTCAC ^GT[ACGT]AC
490 GTCAC
MboI 100 GATC ^GATC
154 GATC
MflI 99 AGATCT [GA]^GATC[CT]
153 AGATCT
MseI 919 TTAA T^TAA
958 TTAA
MvaI 9 CCAGG CC^[AT]GG
279 CCTGG
MvnI 643 CGCG CG^CG
700 CGCG
NdeII 100 GATC ^GATC
154 GATC
NlaIII 234 CATG CATG^
967 CATG
NlaIV 638 GGAGCC GG[ACGT]^[ACGT]CC
925 GGGACC
PvuII 356 CAGCTG CAG^CTG
494 CAGCTG
RsaI 383 GTAC GT^AC
1018 GTAC
Sau3AI 100 GATC ^GATC
154 GATC
Sau96I 896 GGGCC G^G[ACGT]CC
926 GGACC
StuI 659 AGGCCT AGG^CCT
821 AGGCCT
Tru9I 919 TTAA T^TAA
958 TTAA
Tsp45I 364 GTCAC ^GT[GC]AC
490 GTCAC
Tsp509I 134 AATT ^AATT
218 AATT
TspRI 618 CACTG CA[GC]TG[ACGT][ACGT]^
677 CACTG
XcmI 224 CCAAGGCTAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
578 CCAACCCACGCTTGG
XhoII 99 AGATCT [GA]^GATC[CT]
153 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AluI BalI BbsI BbvI
BpuAI BsaJI BseRI BsiYI BslI
BsrI BssKI BstF5I Cac8I EaeI
EcoT14I Fnu4HI FokI HaeIII HphI
ItaI MaeII MboII MluNI MnlI
MscI MspA1I ScrFI StyI TseI