Restriction map for
pF1KB3445
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# NO CUT ENZYMES ( 171 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspHI AspI
AvaI AvaII AviII AvrII BanI
BbeI BbrPI BbsI BbvCI BcgI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DsaI EagI EarI EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MamI MfeI MluI
MroI MseI MspA1I MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tru9I Tsp509I Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AluI 91 AGCT AG^CT
BalI 359 TGGCCA TGG^CCA
BamHI 213 GGATCC G^GATCC
BbvI 443 GCAGC GCAGC(8/12)
BciVI 417 GGATAC GTATCC(6/5)
BcnI 171 CCCGG CC^[GC]GG
BpmI 601 CTCCAG CTGGAG(16/14)
BsaI 220 GAGACC GGTCTC(1/5)
BsaJI 544 CCTTGG C^C[ACGT][ACGT]GG
BsgI 335 GTGCAG GTGCAG(16/14)
Bsp1407I 518 TGTACA T^GTACA
BsrGI 518 TGTACA T^GTACA
BstEII 297 GGTCACC G^GT[ACGT]ACC
BstPI 297 GGTCACC G^GT[ACGT]ACC
BstYI 213 GGATCC [GA]^GATC[CT]
Cac8I 397 GCCCGC GC[ACGT]^[ACGT]GC
DraII 460 GGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 151 GACACAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 447 CCTGAGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 460 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 297 GGTCACC G^GT[ACGT]ACC
EcoT14I 544 CCTTGG C^C[AT][AT]GG
FauI 398 CCCGC CCCGC(4/6)
HapII 172 CCGG C^CGG
HpaII 172 CCGG C^CGG
MflI 213 GGATCC [GA]^GATC[CT]
MluNI 359 TGGCCA TGG^CCA
MscI 359 TGGCCA TGG^CCA
MslI 226 CATTGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 172 CCGG C^CGG
MwoI 534 GCCATTCAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 171 CCCGG CC^[GC]GG
PleI 450 GAGTC GAGTC(4/5)
PstI 441 CTGCAG CTGCA^G
SexAI 301 ACCTGGT A^CC[AT]GGT
SfcI 441 CTGCAG C^T[GA][CT]AG
SspBI 518 TGTACA T^GTACA
StyI 544 CCTTGG C^C[AT][AT]GG
TseI 443 GCAGC G^C[AT]GC
TspRI 522 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 535 CCATTCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 213 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AhdI 222 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
268 GACATACTGTC
AlwI 214 GATCC GGATC(4/5)
213 GGATC
AspEI 222 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
268 GACATACTGTC
BanII 318 GAGCCC G[GA]GC[CT]^C
436 GGGCTC
BmyI 318 GAGCCC G[AGT]GC[ACT]^C
436 GGGCTC
BsiYI 447 CCTGAGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
544 CCTTGGAAGGG
BslI 447 CCTGAGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
544 CCTTGGAAGGG
BsmFI 149 GGGAC GGGAC(10/14)
552 GGGAC
Bsp1286I 318 GAGCCC G[AGT]GC[ACT]^C
436 GGGCTC
BsrBI 132 CCGCTC CCGCTC(-3/-3)
28 GAGCGG
BstF5I 75 CATCC GGATG(2/0)
32 GGATG
DdeI 218 CTGAG C^T[ACGT]AG
448 CTGAG
EaeI 347 TGGCCG [CT]^GGCC[GA]
359 TGGCCA
Eam1105I 222 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
268 GACATACTGTC
Fnu4HI 131 GCCGC GC^[ACGT]GC
443 GCAGC
FokI 75 CATCC GGATG(9/13)
32 GGATG
ItaI 131 GCCGC GC^[ACGT]GC
443 GCAGC
MaeII 186 ACGT A^CGT
374 ACGT
MaeIII 263 GTGAC ^GT[ACGT]AC
298 GTCAC
MboII 471 GAAGA GAAGA(8/7)
389 TCTTC
NlaIII 232 CATG CATG^
477 CATG
Tsp45I 263 GTGAC ^GT[GC]AC
298 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI AfaI BsmAI BssKI BstNI
Cfr13I DpnII EcoRII HaeIII HinfI
HphI MboI MnlI MvaI NdeII
NlaIV RsaI Sau3AI Sau96I ScrFI
TaqI TfiI TthHB8I