Restriction map for
pF1KB5971
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BamHI BbrPI
BciVI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspEI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstXI BstZ17I
CelII Cfr10I CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRI EcoT14I EcoT22I
FseI HindIII HpaI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SbfI
ScaI SexAI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI Tsp509I Tth111I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 1062 GTCGAC GT^[AC][GT]AC
AccII 7 CGCG CG^CG
AcyI 796 GGCGCC G[GA]^CG[CT]C
Alw44I 972 GTGCAC G^TGCAC
AocI 889 CCTGAGG CC^T[ACGT]AGG
ApaLI 972 GTGCAC G^TGCAC
AspHI 972 GTGCAC G[AT]GC[AT]^C
AviII 1391 TGCGCA TGC^GCA
BalI 1295 TGGCCA TGG^CCA
BbeI 796 GGCGCC GGCGC^C
BbvCI 1135 CCTCAGC CCTCAGC(-5/-2)
BcgI 111 CGAGCTGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 575 TGATCA T^GATCA
BsaHI 796 GGCGCC G[GA]^CG[CT]C
BsaI 39 GAGACC GGTCTC(1/5)
BsgI 923 GTGCAG GTGCAG(16/14)
BsiHKAI 972 GTGCAC G[AT]GC[AT]^C
BsmI 640 GAATGC GAATGC(1/-1)
BspDI 730 ATCGAT AT^CGAT
BspHI 1262 TCATGA T^CATGA
BspLU11I 1478 ACATGT A^CATGT
BspMI 775 ACCTGC ACCTGC(4/8)
BsrBI 496 GAGCGG CCGCTC(-3/-3)
BstEII 586 GGTGACC G^GT[ACGT]ACC
BstPI 586 GGTGACC G^GT[ACGT]ACC
BstUI 7 CGCG CG^CG
Bsu36I 889 CCTGAGG CC^T[ACGT]AGG
ClaI 730 ATCGAT AT^CGAT
Eco81I 889 CCTGAGG CC^T[ACGT]AGG
EcoO65I 586 GGTGACC G^GT[ACGT]ACC
EcoRV 124 GATATC GAT^ATC
FbaI 575 TGATCA T^GATCA
FspI 1391 TGCGCA TGC^GCA
HgaI 1065 GACGC GACGC(5/10)
Hin1I 796 GGCGCC G[GA]^CG[CT]C
KasI 796 GGCGCC G^GCGCC
MaeII 461 ACGT A^CGT
MluNI 1295 TGGCCA TGG^CCA
MscI 1295 TGGCCA TGG^CCA
MseI 509 TTAA T^TAA
MspA1I 741 CCGCTG C[AC]G^C[GT]G
MvnI 7 CGCG CG^CG
NarI 796 GGCGCC GG^CGCC
PleI 1275 GACTC GAGTC(4/5)
PshAI 849 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1069 CTGCAG CTGCA^G
RcaI 1262 TCATGA T^CATGA
SalI 1062 GTCGAC G^TCGAC
SapI 1179 GAAGAGC GCTCTTC(1/4)
SfcI 1069 CTGCAG C^T[GA][CT]AG
SfiI 292 GGCCTGAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 796 GGCGCC GGC^GCC
TfiI 33 GATTC G^A[AT]TC
Tru9I 509 TTAA T^TAA
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 461 ACGTGT A^C[GA][CT]GT
1478 ACATGT
AlwI 417 GATCC GGATC(4/5)
1014 GATCC
AvaI 1088 CCCGGG C^[CT]CG[GA]G
1493 CCCGGG
BbsI 441 GAAGAC GAAGAC(2/6)
1272 GAAGAC
BpuAI 441 GAAGAC GAAGAC(2/6)
1272 GAAGAC
BseRI 400 CTCCTC GAGGAG(10/8)
1515 CTCCTC
BsmAI 39 GAGAC GTCTC(1/5)
134 GTCTC
BsmFI 842 GGGAC GGGAC(10/14)
1208 GTCCC
BsoBI 1088 CCCGGG C^[CT]CG[GA]G
1493 CCCGGG
BstYI 416 AGATCC [GA]^GATC[CT]
1013 AGATCC
DsaI 86 CCGTGG C^C[GA][CT]GG
92 CCGTGG
EaeI 89 TGGCCG [CT]^GGCC[GA]
1295 TGGCCA
HaeII 796 GGCGCC [GA]GCGC^[CT]
1255 AGCGCC
HincII 631 GTCAAC GT[CT]^[GA]AC
1062 GTCGAC
HindII 631 GTCAAC GT[CT]^[GA]AC
1062 GTCGAC
HinfI 33 GATTC G^A[ACGT]TC
1275 GACTC
MflI 416 AGATCC [GA]^GATC[CT]
1013 AGATCC
MslI 619 CACTGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
965 CACCTCTGTG
NspI 1099 ACATGC [GA]CATG^[CT]
1478 ACATGT
SmaI 1088 CCCGGG CCC^GGG
1493 CCCGGG
SmlI 930 CTTGAG C^T[CT][GA]AG
1009 CTCAAG
XhoII 416 AGATCC [GA]^GATC[CT]
1013 AGATCC
XmaCI 1088 CCCGGG C^CCGGG
1493 CCCGGG
XmaI 1088 CCCGGG C^CCGGG
1493 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI AlwNI ApaI
AvaII BanI BanII BbvI BcnI
BfaI BglI BmyI BpmI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
StuI TaqI TseI Tsp45I TspRI
TthHB8I XcmI