Restriction map for
pF1KB6453
##################################
# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AocI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BbrPI
BbvCI BcgI BciVI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaWI BseAI BsgI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DrdI DsaI Eam1105I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HgaI HincII HindII
HindIII HpaI KpnI KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MslI MunI MvnI NaeI
NcoI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AcsI 481 GAATTT [GA]^AATT[CT]
AcyI 839 GGCGCC G[GA]^CG[CT]C
AlwNI 325 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 557 AGCGCT AGC^GCT
ApaI 120 GGGCCC GGGCC^C
ApoI 481 GAATTT [GA]^AATT[CT]
Asp700I 568 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 46 GAGCTC G[AT]GC[AT]^C
AvaII 138 GGACC G^G[AT]CC
BbeI 839 GGCGCC GGCGC^C
BbsI 34 GAAGAC GAAGAC(2/6)
BcnI 465 CCGGG CC^[GC]GG
BfaI 946 CTAG C^TAG
BglII 188 AGATCT A^GATCT
BpmI 544 CTGGAG CTGGAG(16/14)
BpuAI 34 GAAGAC GAAGAC(2/6)
BsaHI 839 GGCGCC G[GA]^CG[CT]C
BsiEI 83 CGGCCG CG[GA][CT]^CG
BsiHKAI 46 GAGCTC G[AT]GC[AT]^C
BsmFI 861 GTCCC GGGAC(10/14)
BsrBI 23 GAGCGG CCGCTC(-3/-3)
BsrFI 551 ACCGGC [GA]^CCGG[CT]
BstAPI 732 GCAGGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 551 ACCGGC [GA]^CCGG[CT]
DdeI 49 CTCAG C^T[ACGT]AG
DraIII 161 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 83 CGGCCG [CT]^GGCC[GA]
EagI 83 CGGCCG C^GGCCG
EarI 72 GAAGAG CTCTTC(1/4)
EclXI 83 CGGCCG C^GGCCG
Eco47III 557 AGCGCT AGC^GCT
Eco52I 83 CGGCCG C^GGCCG
FauI 404 CCCGC CCCGC(4/6)
Hin1I 839 GGCGCC G[GA]^CG[CT]C
KasI 839 GGCGCC G^GCGCC
Ksp632I 72 GAAGAG CTCTTC(1/4)
MaeI 946 CTAG C^TAG
MseI 227 TTAA T^TAA
MspA1I 314 CAGCTG C[AC]G^C[GT]G
NarI 839 GGCGCC GG^CGCC
NciI 465 CCGGG CC^[GC]GG
NdeI 509 CATATG CA^TATG
PpuMI 137 AGGACCC [GA]G^G[AT]CC[CT]
PstI 730 CTGCAG CTGCA^G
PvuII 314 CAGCTG CAG^CTG
SacI 46 GAGCTC GAGCT^C
SfcI 730 CTGCAG C^T[GA][CT]AG
SfoI 839 GGCGCC GGC^GCC
SmlI 184 CTCAAG C^T[CT][GA]AG
Tru9I 227 TTAA T^TAA
Tsp45I 887 GTGAC ^GT[GC]AC
XmnI 568 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwI 821 GATCC GGATC(4/5)
897 GATCC
BanI 761 GGCACC G^G[CT][GA]CC
839 GGCGCC
BanII 46 GAGCTC G[GA]GC[CT]^C
120 GGGCCC
BclI 453 TGATCA T^GATCA
921 TGATCA
BsaI 286 GAGACC GGTCTC(1/5)
626 GAGACC
BseRI 442 GAGGAG GAGGAG(10/8)
445 GAGGAG
BsmAI 286 GAGAC GTCTC(1/5)
626 GAGAC
BsmI 684 GAATGC GAATGC(1/-1)
813 GAATGC
BspMI 174 ACCTGC ACCTGC(4/8)
876 ACCTGC
BstYI 188 AGATCT [GA]^GATC[CT]
896 AGATCC
EcoNI 129 CCTGTATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
517 CCTAATGCAGG
FbaI 453 TGATCA T^GATCA
921 TGATCA
HapII 465 CCGG C^CGG
552 CCGG
HinfI 338 GACTC G^A[ACGT]TC
487 GACTC
HpaII 465 CCGG C^CGG
552 CCGG
MflI 188 AGATCT [GA]^GATC[CT]
896 AGATCC
MspI 465 CCGG C^CGG
552 CCGG
NlaIII 451 CATG CATG^
643 CATG
PleI 338 GACTC GAGTC(4/5)
487 GACTC
TaqI 216 TCGA T^CGA
819 TCGA
TthHB8I 216 TCGA T^CGA
819 TCGA
XhoII 188 AGATCT [GA]^GATC[CT]
896 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AfaI AluI BbvI BmyI
BsaJI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DpnII DraII EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HhaI HinP1I HphI ItaI MaeIII
MboI MboII MnlI MvaI MwoI
NdeII NlaIV RsaI Sau3AI Sau96I
ScrFI StyI TseI Tsp509I TspRI