Restriction map for
pF1KE0213
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BcnI
BfaI BfrI BglI BglII BlnI
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstEII
BstPI BstXI Bsu36I Cfr10I Cfr13I
ClaI CpoI DraI DraII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FseI FspI HaeIII HapII HgaI
Hin1I HindIII HpaI HpaII KasI
KpnI KspI MaeI MamI MfeI
MluI MluNI MroI MscI MslI
MspI MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI Sau96I SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TfiI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 737 GTATAC GT^[AC][GT]AC
AccII 679 CGCG CG^CG
AlwI 189 GGATC GGATC(4/5)
AseI 237 ATTAAT AT^TAAT
AsnI 237 ATTAAT AT^TAAT
Asp700I 64 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BclI 795 TGATCA T^GATCA
BlpI 142 GCTCAGC GC^T[ACGT]AGC
BmyI 93 GGGCAC G[AGT]GC[ACT]^C
BpmI 545 CTGGAG CTGGAG(16/14)
Bpu1102I 142 GCTCAGC GC^T[ACGT]AGC
BsaJI 36 CCCTGG C^C[ACGT][ACGT]GG
BseRI 103 GAGGAG GAGGAG(10/8)
BsiYI 487 CCTGATGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 487 CCTGATGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 93 GGGCAC G[AGT]GC[ACT]^C
BspMI 741 ACCTGC ACCTGC(4/8)
BsrBI 49 GAGCGG CCGCTC(-3/-3)
BsrI 220 ACTGG ACTGG(1/-1)
Bst1107I 737 GTATAC GTA^TAC
BstBI 731 TTCGAA TT^CGAA
BstUI 679 CGCG CG^CG
BstYI 189 GGATCT [GA]^GATC[CT]
BstZ17I 737 GTATAC GTA^TAC
Cac8I 51 GCGGGC GC[ACGT]^[ACGT]GC
CelII 142 GCTCAGC GC^T[ACGT]AGC
CfoI 147 GCGC GCG^C
DraIII 599 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
FauI 51 GCGGG CCCGC(4/6)
FbaI 795 TGATCA T^GATCA
HaeII 146 AGCGCC [GA]GCGC^[CT]
HhaI 147 GCGC GCG^C
HinP1I 147 GCGC G^CGC
HincII 123 GTTGAC GT[CT]^[GA]AC
HindII 123 GTTGAC GT[CT]^[GA]AC
HphI 584 TCACC GGTGA(8/7)
MaeII 419 ACGT A^CGT
MaeIII 25 GTGAC ^GT[ACGT]AC
MflI 189 GGATCT [GA]^GATC[CT]
MspA1I 448 CAGCGG C[AC]G^C[GT]G
MvnI 679 CGCG CG^CG
MwoI 745 GCCTACCTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 291 ACATGC [GA]CATG^[CT]
NspV 731 TTCGAA TT^CGAA
PshBI 237 ATTAAT AT^TAAT
PstI 161 CTGCAG CTGCA^G
SapI 931 GCTCTTC GCTCTTC(1/4)
ScaI 324 AGTACT AGT^ACT
SfcI 161 CTGCAG C^T[GA][CT]AG
SfuI 731 TTCGAA TT^CGAA
TaqI 732 TCGA T^CGA
Tsp45I 25 GTGAC ^GT[GC]AC
TspRI 21 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 732 TCGA T^CGA
XhoII 189 GGATCT [GA]^GATC[CT]
XmnI 64 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 10 enzymes) #
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AcsI 226 AAATTT [GA]^AATT[CT]
870 GAATTT
ApoI 226 AAATTT [GA]^AATT[CT]
870 GAATTT
BbvI 163 GCAGC GCAGC(8/12)
939 GCTGC
BsaI 482 GAGACC GGTCTC(1/5)
757 GAGACC
EarI 68 CTCTTC CTCTTC(1/4)
932 CTCTTC
Fnu4HI 163 GCAGC GC^[ACGT]GC
939 GCTGC
ItaI 163 GCAGC GC^[ACGT]GC
939 GCTGC
Ksp632I 68 CTCTTC CTCTTC(1/4)
932 CTCTTC
SmlI 402 CTCAAG C^T[CT][GA]AG
509 CTCAAG
TseI 163 GCAGC G^C[AT]GC
939 GCTGC
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI AfaI AluI BsmAI BsmFI
BssKI BstF5I BstNI DdeI DpnII
EcoRII FokI HinfI MboI MboII
MnlI MseI MvaI NdeII NlaIII
PleI RsaI Sau3AI ScrFI SfaNI
Tru9I Tsp509I