Restriction map for
pF1KB6464
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
Acc65I AccI AccII AclI AflII
AflIII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BbsI BcgI BciVI BclI BfaI
BfrI BglII BlnI BpuAI BsaAI
BsaBI BsaI BsiWI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
ClaI CpoI DraI DraIII DrdI
Eam1105I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MaeI MamI MfeI MluI MunI
MvnI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI SspBI SspI StuI
SwaI TaqI Tth111I TthHB8I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AatII 279 GACGTC GACGT^C
AccIII 608 TCCGGA T^CCGGA
AcsI 409 AAATTC [GA]^AATT[CT]
AlwNI 302 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 523 CCTGAGG CC^T[ACGT]AGG
Aor51HI 52 AGCGCT AGC^GCT
ApoI 409 AAATTC [GA]^AATT[CT]
Asp700I 972 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 195 GAGCAC G[AT]GC[AT]^C
BalI 140 TGGCCA TGG^CCA
BanI 459 GGCGCC G^G[CT][GA]CC
BbeI 459 GGCGCC GGCGC^C
BbvCI 468 GCTGAGG CCTCAGC(-5/-2)
BcnI 876 CCGGG CC^[GC]GG
BlpI 30 GCTGAGC GC^T[ACGT]AGC
BpmI 284 CTGGAG CTGGAG(16/14)
Bpu1102I 30 GCTGAGC GC^T[ACGT]AGC
BsaWI 608 TCCGGA [AT]^CCGG[AT]
BseAI 608 TCCGGA T^CCGGA
BsiEI 707 CGGCCG CG[GA][CT]^CG
BsiHKAI 195 GAGCAC G[AT]GC[AT]^C
BsmBI 277 GAGACG CGTCTC(1/5)
BspEI 608 TCCGGA T^CCGGA
BsrFI 709 GCCGGT [GA]^CCGG[CT]
BstAPI 910 GCATTTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 116 GGATCT [GA]^GATC[CT]
Bsu36I 523 CCTGAGG CC^T[ACGT]AGG
CelII 30 GCTGAGC GC^T[ACGT]AGC
Cfr10I 709 GCCGGT [GA]^CCGG[CT]
EagI 707 CGGCCG C^GGCCG
EclXI 707 CGGCCG C^GGCCG
Eco47III 52 AGCGCT AGC^GCT
Eco52I 707 CGGCCG C^GGCCG
Eco81I 523 CCTGAGG CC^T[ACGT]AGG
FauI 456 GCGGG CCCGC(4/6)
HgaI 935 GCGTC GACGC(5/10)
KasI 459 GGCGCC G^GCGCC
MaeII 280 ACGT A^CGT
MflI 116 GGATCT [GA]^GATC[CT]
MluNI 140 TGGCCA TGG^CCA
MroI 608 TCCGGA T^CCGGA
MscI 140 TGGCCA TGG^CCA
MseI 595 TTAA T^TAA
NarI 459 GGCGCC GG^CGCC
NciI 876 CCGGG CC^[GC]GG
PflMI 507 CCAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SbfI 483 CCTGCAGG CCTGCA^GG
ScaI 1004 AGTACT AGT^ACT
SfoI 459 GGCGCC GGC^GCC
Sse8387I 483 CCTGCAGG CCTGCA^GG
TfiI 685 GATTC G^A[AT]TC
Tru9I 595 TTAA T^TAA
Tsp509I 410 AATT ^AATT
TspRI 191 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 507 CCAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 533 CCATCTTCACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 116 GGATCT [GA]^GATC[CT]
XmnI 972 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 279 GACGTC G[GA]^CG[CT]C
459 GGCGCC
AlwI 116 GGATC GGATC(4/5)
640 GGATC
ApaI 14 GGGCCC GGGCC^C
879 GGGCCC
AvaI 10 CTCGGG C^[CT]CG[GA]G
600 CCCGAG
BglI 37 GCCTGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
692 GCCAGCTGGGC
BsaHI 279 GACGTC G[GA]^CG[CT]C
459 GGCGCC
BsgI 91 GTGCAG GTGCAG(16/14)
996 GTGCAG
BsmAI 277 GAGAC GTCTC(1/5)
769 GAGAC
BsoBI 10 CTCGGG C^[CT]CG[GA]G
600 CCCGAG
BsrI 657 ACTGG ACTGG(1/-1)
777 CCAGT
EaeI 140 TGGCCA [CT]^GGCC[GA]
707 CGGCCG
EarI 940 CTCTTC CTCTTC(1/4)
631 GAAGAG
Hin1I 279 GACGTC G[GA]^CG[CT]C
459 GGCGCC
Ksp632I 940 CTCTTC CTCTTC(1/4)
631 GAAGAG
MspA1I 466 CAGCTG C[AC]G^C[GT]G
694 CAGCTG
PstI 484 CTGCAG CTGCA^G
832 CTGCAG
PvuII 466 CAGCTG CAG^CTG
694 CAGCTG
SfaNI 916 GATGC GCATC(5/9)
518 GCATC
Tsp45I 670 GTCAC ^GT[GC]AC
814 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AvaII BanII
BbvI BmyI BsaJI BseRI BsiYI
BslI BsmFI Bsp1286I BspMI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MnlI MslI MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfcI StyI TseI