Restriction map for
pF1KB5369
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbvCI
BcgI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrGI BssHII Bst1107I BstBI BstZ17I
CelII ClaI CpoI DraI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV FseI FspI HindIII HinfI
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MroI MseI
MspA1I MunI NarI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PleI PmeI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 1539 CGCG CG^CG
AcyI 1417 GGCGTC G[GA]^CG[CT]C
AflIII 674 ACATGT A^C[GA][CT]GT
AlwI 954 GATCC GGATC(4/5)
AocI 580 CCTGAGG CC^T[ACGT]AGG
ApaI 1392 GGGCCC GGGCC^C
AspHI 1490 GTGCTC G[AT]GC[AT]^C
BanII 1392 GGGCCC G[GA]GC[CT]^C
BbrPI 1444 CACGTG CAC^GTG
BciVI 1159 GGATAC GTATCC(6/5)
BclI 650 TGATCA T^GATCA
BsaAI 1444 CACGTG [CT]AC^GT[GA]
BsaHI 1417 GGCGTC G[GA]^CG[CT]C
BsiHKAI 1490 GTGCTC G[AT]GC[AT]^C
BspLU11I 674 ACATGT A^CATGT
BsrDI 1247 GCAATG GCAATG(2/0)
BsrFI 331 GCCGGC [GA]^CCGG[CT]
BstEII 438 GGTCACC G^GT[ACGT]ACC
BstPI 438 GGTCACC G^GT[ACGT]ACC
BstUI 1539 CGCG CG^CG
BstXI 234 CCACAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 953 AGATCC [GA]^GATC[CT]
Bsu36I 580 CCTGAGG CC^T[ACGT]AGG
Cfr10I 331 GCCGGC [GA]^CCGG[CT]
DraIII 841 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 580 CCTGAGG CC^T[ACGT]AGG
EcoNI 1044 CCTGCATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 438 GGTCACC G^GT[ACGT]ACC
EcoT22I 625 ATGCAT ATGCA^T
FbaI 650 TGATCA T^GATCA
Hin1I 1417 GGCGTC G[GA]^CG[CT]C
HincII 757 GTTGAC GT[CT]^[GA]AC
HindII 757 GTTGAC GT[CT]^[GA]AC
MflI 953 AGATCC [GA]^GATC[CT]
MvnI 1539 CGCG CG^CG
NaeI 331 GCCGGC GCC^GGC
NgoMIV 331 GCCGGC G^CCGGC
NsiI 625 ATGCAT ATGCA^T
PmaCI 1444 CACGTG CAC^GTG
PmlI 1444 CACGTG CAC^GTG
PpuMI 317 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 1116 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1520 CTGCAG CTGCA^G
SapI 768 GAAGAGC GCTCTTC(1/4)
SfcI 1520 CTGCAG C^T[GA][CT]AG
StuI 584 AGGCCT AGG^CCT
TaqI 869 TCGA T^CGA
Tsp509I 1005 AATT ^AATT
TthHB8I 869 TCGA T^CGA
XhoII 953 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Asp700I 768 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1221 GAAGCCCTTC
BalI 23 TGGCCA TGG^CCA
944 TGGCCA
BpmI 121 CTGGAG CTGGAG(16/14)
259 CTGGAG
BseRI 1098 CTCCTC GAGGAG(10/8)
247 GAGGAG
BsgI 451 CTGCAC GTGCAG(16/14)
1411 CTGCAC
BspMI 34 ACCTGC ACCTGC(4/8)
1406 ACCTGC
BstAPI 1038 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1404 GCACCTGCTGC
DpnII 651 GATC ^GATC
954 GATC
DrdI 912 GACGGACCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1372 GACGTGGCCGTC
EarI 865 CTCTTC CTCTTC(1/4)
768 GAAGAG
FauI 209 CCCGC CCCGC(4/6)
1364 GCGGG
HaeII 328 AGCGCC [GA]GCGC^[CT]
523 AGCGCC
Ksp632I 865 CTCTTC CTCTTC(1/4)
768 GAAGAG
MboI 651 GATC ^GATC
954 GATC
MluNI 23 TGGCCA TGG^CCA
944 TGGCCA
MscI 23 TGGCCA TGG^CCA
944 TGGCCA
NcoI 1295 CCATGG C^CATGG
1397 CCATGG
NdeII 651 GATC ^GATC
954 GATC
NspI 674 ACATGT [GA]CATG^[CT]
881 ACATGC
Sau3AI 651 GATC ^GATC
954 GATC
ScaI 18 AGTACT AGT^ACT
80 AGTACT
SmlI 217 CTCAAG C^T[CT][GA]AG
588 CTTGAG
XmnI 768 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1221 GAAGCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BanI BbsI BbvI BcnI BglI
BmyI BpuAI BsaI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BssSI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DraII DsaI
EaeI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HpaII HphI ItaI MaeII
MaeIII MboII MnlI MslI MspI
MvaI MwoI NciI NlaIII NlaIV
PflMI RsaI Sau96I ScrFI SfaNI
StyI TseI Tsp45I TspRI Van91I
XcmI