Restriction map for
pF1KE0336
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# NO CUT ENZYMES ( 126 enzymes) #
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Acc65I AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AviII AvrII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaWI BseAI BsiHKAI
BsiWI BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI Eam1105I
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KasI KpnI MaeI MamI
MfeI MluI MroI MseI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI ScaI
SfaNI SfoI SfuI SgrAI SmaI
SnaBI SpeI SspI StuI SwaI
TaqI TfiI Tru9I Tsp509I Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 1162 GACGTC GACGT^C
AccI 850 GTAGAC GT^[AC][GT]AC
ApaI 506 GGGCCC GGGCC^C
Asp700I 605 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 205 CTCGGG C^[CT]CG[GA]G
BamHI 650 GGATCC G^GATCC
BanI 1146 GGTGCC G^G[CT][GA]CC
BsaAI 247 TACGTG [CT]AC^GT[GA]
BsaI 1031 GGTCTC GGTCTC(1/5)
BsiEI 1456 CGGCCG CG[GA][CT]^CG
BsmBI 354 GAGACG CGTCTC(1/5)
BsmI 1062 GAATGC GAATGC(1/-1)
BsoBI 205 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1019 TGTACA T^GTACA
BsrDI 1123 GCAATG GCAATG(2/0)
BsrGI 1019 TGTACA T^GTACA
BstAPI 1128 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 650 GGATCC [GA]^GATC[CT]
DraIII 1051 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1197 GACAGGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1456 CGGCCG C^GGCCG
EarI 829 CTCTTC CTCTTC(1/4)
EclXI 1456 CGGCCG C^GGCCG
Eco52I 1456 CGGCCG C^GGCCG
EcoNI 1296 CCTCATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 1228 AGCGCC [GA]GCGC^[CT]
HincII 1222 GTCAAC GT[CT]^[GA]AC
HindII 1222 GTCAAC GT[CT]^[GA]AC
Ksp632I 829 CTCTTC CTCTTC(1/4)
MboII 830 TCTTC GAAGA(8/7)
MflI 650 GGATCC [GA]^GATC[CT]
NcoI 371 CCATGG C^CATGG
NspI 1274 GCATGC [GA]CATG^[CT]
PshAI 1158 GACAGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1141 CAGCTG CAG^CTG
SapI 828 GCTCTTC GCTCTTC(1/4)
SbfI 114 CCTGCAGG CCTGCA^GG
SexAI 71 ACCTGGT A^CC[AT]GGT
SmlI 172 CTTGAG C^T[CT][GA]AG
SphI 1274 GCATGC GCATG^C
Sse8387I 114 CCTGCAGG CCTGCA^GG
SspBI 1019 TGTACA T^GTACA
Tsp45I 397 GTGAC ^GT[GC]AC
XcmI 62 CCACTGTCCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 650 GGATCC [GA]^GATC[CT]
XmnI 605 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcyI 1117 GGCGTC G[GA]^CG[CT]C
1162 GACGTC
AlwI 651 GATCC GGATC(4/5)
650 GGATC
BalI 377 TGGCCA TGG^CCA
1352 TGGCCA
BsaHI 1117 GGCGTC G[GA]^CG[CT]C
1162 GACGTC
BseRI 100 GAGGAG GAGGAG(10/8)
299 GAGGAG
BssSI 499 CACGAG CACGAG(-5/-1)
1054 CTCGTG
BstXI 446 CCAATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1355 CCAGCGTCGTGG
CfoI 868 GCGC GCG^C
1229 GCGC
DraII 766 GGGACCT [GA]G^G[ACGT]CC[CT]
1346 GGGTCCT
EcoO109I 766 GGGACCT [GA]G^G[ACGT]CC[CT]
1346 GGGTCCT
EcoT14I 371 CCATGG C^C[AT][AT]GG
593 CCTTGG
HhaI 868 GCGC GCG^C
1229 GCGC
Hin1I 1117 GGCGTC G[GA]^CG[CT]C
1162 GACGTC
HinP1I 868 GCGC G^CGC
1229 GCGC
KspI 239 CCGCGG CCGC^GG
807 CCGCGG
MaeIII 339 GTTAC ^GT[ACGT]AC
397 GTGAC
MluNI 377 TGGCCA TGG^CCA
1352 TGGCCA
MscI 377 TGGCCA TGG^CCA
1352 TGGCCA
PpuMI 766 GGGACCT [GA]G^G[AT]CC[CT]
1346 GGGTCCT
SacII 239 CCGCGG CCGC^GG
807 CCGCGG
SfiI 369 GGCCATGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
477 GGCCCTCGCGGCC
StyI 371 CCATGG C^C[AT][AT]GG
593 CCTTGG
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# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AvaII BanII BbvI BcnI BglI
BmyI BpmI BsaJI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
Cac8I Cfr13I DdeI DpnII DsaI
EaeI EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HinfI HpaII
HphI ItaI MaeII MboI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfcI TseI TspRI