Restriction map for
pF1KB6348
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Aor51HI
ApaI AscI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmAI BsmBI BsmI
BsoBI BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HapII HhaI
HinP1I HindIII HpaI HpaII KasI
KpnI KspI MfeI MflI MluI
MluNI MroI MscI MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 21 GTCTAC GT^[AC][GT]AC
AcsI 244 GAATTT [GA]^AATT[CT]
AcyI 277 GGCGTC G[GA]^CG[CT]C
Alw44I 657 GTGCAC G^TGCAC
AlwI 454 GATCC GGATC(4/5)
AlwNI 587 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 461 CCTCAGG CC^T[ACGT]AGG
ApaLI 657 GTGCAC G^TGCAC
ApoI 244 GAATTT [GA]^AATT[CT]
Asp700I 3 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 657 GTGCAC G[AT]GC[AT]^C
AvaII 211 GGACC G^G[AT]CC
BfaI 448 CTAG C^TAG
BmyI 657 GTGCAC G[AGT]GC[ACT]^C
BpmI 12 CTGGAG CTGGAG(16/14)
BsaBI 388 GATAACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 277 GGCGTC G[GA]^CG[CT]C
BsiHKAI 657 GTGCAC G[AT]GC[AT]^C
BsmFI 74 GGGAC GGGAC(10/14)
Bsp1286I 657 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 732 TGTACA T^GTACA
BspMI 525 GCAGGT ACCTGC(4/8)
BsrGI 732 TGTACA T^GTACA
BsrI 86 CCAGT ACTGG(1/-1)
Bsu36I 461 CCTCAGG CC^T[ACGT]AGG
Cfr13I 211 GGACC G^G[ACGT]CC
DraI 625 TTTAAA TTT^AAA
DsaI 718 CCATGG C^C[GA][CT]GG
Eco81I 461 CCTCAGG CC^T[ACGT]AGG
FauI 444 CCCGC CCCGC(4/6)
HaeIII 814 GGCC GG^CC
Hin1I 277 GGCGTC G[GA]^CG[CT]C
MaeI 448 CTAG C^TAG
MaeII 77 ACGT A^CGT
MaeIII 404 GTGAC ^GT[ACGT]AC
MamI 388 GATAACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 308 CATTAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 115 CAGCTG C[AC]G^C[GT]G
NcoI 718 CCATGG C^CATGG
PleI 762 GACTC GAGTC(4/5)
PstI 593 CTGCAG CTGCA^G
PvuII 115 CAGCTG CAG^CTG
Sau96I 211 GGACC G^G[ACGT]CC
ScaI 597 AGTACT AGT^ACT
SfaNI 756 GATGC GCATC(5/9)
SfcI 593 CTGCAG C^T[GA][CT]AG
SphI 198 GCATGC GCATG^C
SspBI 732 TGTACA T^GTACA
SspI 327 AATATT AAT^ATT
TaqI 425 TCGA T^CGA
TfiI 723 GAATC G^A[AT]TC
Tsp45I 404 GTGAC ^GT[GC]AC
TthHB8I 425 TCGA T^CGA
XmnI 3 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AciI 445 CCGC CCGC(-3/-1)
504 GCGG
AseI 309 ATTAAT AT^TAAT
531 ATTAAT
AsnI 309 ATTAAT AT^TAAT
531 ATTAAT
BbsI 60 GAAGAC GAAGAC(2/6)
691 GAAGAC
BpuAI 60 GAAGAC GAAGAC(2/6)
691 GAAGAC
BsaJI 251 CCTTGG C^C[ACGT][ACGT]GG
718 CCATGG
BsgI 101 CTGCAC GTGCAG(16/14)
286 GTGCAG
BsiYI 457 CCCACCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
816 CCTGTCATGGG
BslI 457 CCCACCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
816 CCTGTCATGGG
BstF5I 381 CATCC GGATG(2/0)
474 GGATG
DpnII 44 GATC ^GATC
454 GATC
EcoT14I 251 CCTTGG C^C[AT][AT]GG
718 CCATGG
FokI 381 CATCC GGATG(9/13)
474 GGATG
HgaI 202 GCGTC GACGC(5/10)
278 GCGTC
HincII 183 GTCAAC GT[CT]^[GA]AC
640 GTTGAC
HindII 183 GTCAAC GT[CT]^[GA]AC
640 GTTGAC
HinfI 723 GAATC G^A[ACGT]TC
762 GACTC
MboI 44 GATC ^GATC
454 GATC
MwoI 29 GCTCTGTACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
169 GCCCCAGCAGC
NdeII 44 GATC ^GATC
454 GATC
NlaIV 211 GGACCC GG[ACGT]^[ACGT]CC
506 GGAACC
NspI 17 GCATGT [GA]CATG^[CT]
198 GCATGC
PshBI 309 ATTAAT AT^TAAT
531 ATTAAT
Sau3AI 44 GATC ^GATC
454 GATC
StyI 251 CCTTGG C^C[AT][AT]GG
718 CCATGG
Tsp509I 245 AATT ^AATT
705 AATT
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AfaI AluI BbvI BssKI BstNI
Cac8I DdeI EarI EcoRII Fnu4HI
HphI ItaI Ksp632I MboII MnlI
MseI MvaI NlaIII RsaI ScrFI
Tru9I TseI TspRI