Restriction map for
pF1KE1082
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccIII AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BalI BamHI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspEI BspHI BspLU11I BsrDI BsrFI
BsrGI BssSI Bst1107I BstAPI BstBI
BstXI BstZ17I Bsu36I CelII Cfr10I
CpoI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoNI EcoRI EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI MamI MfeI
MluI MluNI MroI MscI MslI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI RcaI RsrII SacI SalI
SapI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI StuI SwaI Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AclI 10 AACGTT AA^CGTT
AcsI 154 AAATTC [GA]^AATT[CT]
AcyI 129 GGCGCC G[GA]^CG[CT]C
AfaI 528 GTAC GT^AC
ApoI 154 AAATTC [GA]^AATT[CT]
Asp700I 150 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 715 GTGCTC G[AT]GC[AT]^C
AvaI 703 CCCGAG C^[CT]CG[GA]G
BbeI 129 GGCGCC GGCGC^C
BbsI 580 GTCTTC GAAGAC(2/6)
BfaI 188 CTAG C^TAG
BpuAI 580 GTCTTC GAAGAC(2/6)
BsaHI 129 GGCGCC G[GA]^CG[CT]C
BsaI 364 GAGACC GGTCTC(1/5)
BsgI 55 GTGCAG GTGCAG(16/14)
BsiHKAI 715 GTGCTC G[AT]GC[AT]^C
BsmFI 183 GTCCC GGGAC(10/14)
BsoBI 703 CCCGAG C^[CT]CG[GA]G
BspDI 784 ATCGAT AT^CGAT
BspMI 530 ACCTGC ACCTGC(4/8)
BsrI 882 ACTGG ACTGG(1/-1)
BssHII 463 GCGCGC G^CGCGC
BstEII 303 GGTGACC G^GT[ACGT]ACC
BstPI 303 GGTGACC G^GT[ACGT]ACC
ClaI 784 ATCGAT AT^CGAT
EarI 1246 GAAGAG CTCTTC(1/4)
EcoO65I 303 GGTGACC G^GT[ACGT]ACC
EcoRV 142 GATATC GAT^ATC
EcoT14I 1172 CCAAGG C^C[AT][AT]GG
HgaI 506 GCGTC GACGC(5/10)
Hin1I 129 GGCGCC G[GA]^CG[CT]C
KasI 129 GGCGCC G^GCGCC
Ksp632I 1246 GAAGAG CTCTTC(1/4)
KspI 495 CCGCGG CCGC^GG
MaeI 188 CTAG C^TAG
MseI 1187 TTAA T^TAA
NarI 129 GGCGCC GG^CGCC
PpuMI 1036 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 10 AACGTT AA^CGTT
PvuII 292 CAGCTG CAG^CTG
RsaI 528 GTAC GT^AC
SacII 495 CCGCGG CCGC^GG
SbfI 531 CCTGCAGG CCTGCA^GG
SfaNI 563 GCATC GCATC(5/9)
SfoI 129 GGCGCC GGC^GCC
SmlI 889 CTCAAG C^T[CT][GA]AG
Sse8387I 531 CCTGCAGG CCTGCA^GG
StyI 1172 CCAAGG C^C[AT][AT]GG
TfiI 661 GATTC G^A[AT]TC
Tru9I 1187 TTAA T^TAA
Tsp509I 155 AATT ^AATT
XmnI 150 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 464 CGCG CG^CG
496 CGCG
AspI 678 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
982 GACAGTGTC
AvaII 885 GGTCC G^G[AT]CC
1037 GGTCC
BanI 129 GGCGCC G^G[CT][GA]CC
896 GGTGCC
BanII 202 GGGCTC G[GA]GC[CT]^C
706 GAGCCC
BsiEI 284 CGGCCG CG[GA][CT]^CG
416 CGGCCG
BsmBI 432 CGTCTC CGTCTC(1/5)
903 CGTCTC
BsrBI 195 CCGCTC CCGCTC(-3/-3)
405 GAGCGG
BstF5I 387 CATCC GGATG(2/0)
1206 CATCC
BstUI 464 CGCG CG^CG
496 CGCG
BstYI 537 GGATCT [GA]^GATC[CT]
1139 AGATCC
DraII 1036 AGGTCCT [GA]G^G[ACGT]CC[CT]
1166 AGGCCCC
DsaI 281 CCACGG C^C[GA][CT]GG
495 CCGCGG
EagI 284 CGGCCG C^GGCCG
416 CGGCCG
EclXI 284 CGGCCG C^GGCCG
416 CGGCCG
Eco52I 284 CGGCCG C^GGCCG
416 CGGCCG
EcoO109I 1036 AGGTCCT [GA]G^G[ACGT]CC[CT]
1166 AGGCCCC
FokI 387 CATCC GGATG(9/13)
1206 CATCC
MflI 537 GGATCT [GA]^GATC[CT]
1139 AGATCC
MvnI 464 CGCG CG^CG
496 CGCG
NlaIII 327 CATG CATG^
721 CATG
PflFI 678 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
982 GACAGTGTC
PstI 118 CTGCAG CTGCA^G
532 CTGCAG
SfcI 118 CTGCAG C^T[GA][CT]AG
532 CTGCAG
TspRI 881 CACTG CA[GC]TG[ACGT][ACGT]^
984 CAGTG
Tth111I 678 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
982 GACAGTGTC
XhoII 537 GGATCT [GA]^GATC[CT]
1139 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AlwI AlwNI BbvI
BcnI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI Bsp1286I BssKI
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIV PleI Sau3AI Sau96I ScrFI
TaqI TseI Tsp45I TthHB8I