Restriction map for
pF1KB7932
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AvaI AvaII
AviII AvrII BalI BamHI BbrPI
BbvCI BcgI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstXI Bsu36I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI Eam1105I Eco47III Eco81I
EcoNI EcoO109I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HindIII
KpnI MaeII MamI MfeI MluI
MluNI MscI MunI NaeI NcoI
NdeI NgoMIV NheI NlaIII NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SalI SbfI ScaI SexAI SfcI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 465 GTATAC GT^[AC][GT]AC
AccIII 497 TCCGGA T^CCGGA
AfaI 66 GTAC GT^AC
AluI 331 AGCT AG^CT
AspI 535 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 208 GAGCCC G[GA]GC[CT]^C
BfaI 505 CTAG C^TAG
BglI 255 GCCCGTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 558 AGATCT A^GATCT
BmyI 208 GAGCCC G[AGT]GC[ACT]^C
BsaWI 497 TCCGGA [AT]^CCGG[AT]
BseAI 497 TCCGGA T^CCGGA
BsgI 580 GTGCAG GTGCAG(16/14)
BsiEI 172 CGGCCG CG[GA][CT]^CG
BsmAI 528 GAGAC GTCTC(1/5)
Bsp1286I 208 GAGCCC G[AGT]GC[ACT]^C
BspEI 497 TCCGGA T^CCGGA
BsrBI 595 GAGCGG CCGCTC(-3/-3)
BsrDI 615 CATTGC GCAATG(2/0)
Bst1107I 465 GTATAC GTA^TAC
BstEII 340 GGTCACC G^GT[ACGT]ACC
BstPI 340 GGTCACC G^GT[ACGT]ACC
BstYI 558 AGATCT [GA]^GATC[CT]
BstZ17I 465 GTATAC GTA^TAC
DdeI 515 CTGAG C^T[ACGT]AG
DpnII 559 GATC ^GATC
DsaI 175 CCGCGG C^C[GA][CT]GG
EaeI 172 CGGCCG [CT]^GGCC[GA]
EagI 172 CGGCCG C^GGCCG
EclXI 172 CGGCCG C^GGCCG
Eco52I 172 CGGCCG C^GGCCG
EcoO65I 340 GGTCACC G^GT[ACGT]ACC
FauI 86 GCGGG CCCGC(4/6)
HpaI 388 GTTAAC GTT^AAC
KspI 175 CCGCGG CCGC^GG
MaeI 505 CTAG C^TAG
MaeIII 341 GTCAC ^GT[ACGT]AC
MboI 559 GATC ^GATC
MflI 558 AGATCT [GA]^GATC[CT]
MroI 497 TCCGGA T^CCGGA
MseI 389 TTAA T^TAA
MslI 571 CACCCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 175 CCGCGG C[AC]G^C[GT]G
NdeII 559 GATC ^GATC
NotI 171 GCGGCCGC GC^GGCCGC
PflFI 535 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsaI 66 GTAC GT^AC
SacII 175 CCGCGG CCGC^GG
SapI 351 GCTCTTC GCTCTTC(1/4)
Sau3AI 559 GATC ^GATC
Tru9I 389 TTAA T^TAA
Tsp45I 341 GTCAC ^GT[GC]AC
Tth111I 535 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 558 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BanI 100 GGCGCC G^G[CT][GA]CC
145 GGCGCC
BbeI 100 GGCGCC GGCGC^C
145 GGCGCC
BbsI 79 GAAGAC GAAGAC(2/6)
565 GAAGAC
BpmI 487 CTCCAG CTGGAG(16/14)
592 CTGGAG
BpuAI 79 GAAGAC GAAGAC(2/6)
565 GAAGAC
BsiYI 6 CCGGAGGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
307 CCTGGCACGGG
BslI 6 CCGGAGGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
307 CCTGGCACGGG
BsmFI 23 GGGAC GGGAC(10/14)
367 GGGAC
BstF5I 324 CATCC GGATG(2/0)
629 GGATG
FokI 324 CATCC GGATG(9/13)
629 GGATG
HaeII 100 GGCGCC [GA]GCGC^[CT]
145 GGCGCC
HgaI 56 GCGTC GACGC(5/10)
245 GCGTC
HincII 388 GTTAAC GT[CT]^[GA]AC
424 GTTGAC
HindII 388 GTTAAC GT[CT]^[GA]AC
424 GTTGAC
HphI 195 GGTGA GGTGA(8/7)
342 TCACC
KasI 100 GGCGCC G^GCGCC
145 GGCGCC
NarI 100 GGCGCC GG^CGCC
145 GGCGCC
SfoI 100 GGCGCC GGC^GCC
145 GGCGCC
TaqI 59 TCGA T^CGA
272 TCGA
Tsp509I 401 AATT ^AATT
520 AATT
TspRI 455 CACTG CA[GC]TG[ACGT][ACGT]^
461 CAGTG
TthHB8I 59 TCGA T^CGA
272 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AccII AciI AcyI BbvI BcnI
BsaHI BsaJI BseRI BsrI BssKI
BstNI BstUI Cac8I CfoI Cfr13I
EarI EcoRII Fnu4HI HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
ItaI Ksp632I MboII MnlI MspI
MvaI MvnI MwoI NciI NlaIV
PleI Sau96I ScrFI SfaNI TseI