Restriction map for
pF1KB6271
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# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AhdI ApoI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BamHI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BsaBI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrGI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HindIII HpaI
KpnI MamI MfeI MluI MroI
MseI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PmeI PshAI PshBI Psp1406I
PvuI RsrII SalI SexAI SfuI
SmaI SmlI SnaBI SpeI SphI
SspBI SspI SwaI Tru9I Tsp509I
Tth111I XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 366 GTCTAC GT^[AC][GT]AC
AgeI 1517 ACCGGT A^CCGGT
AocI 765 CCTGAGG CC^T[ACGT]AGG
AscI 684 GGCGCGCC GG^CGCGCC
AviII 820 TGCGCA TGC^GCA
BalI 1343 TGGCCA TGG^CCA
BbrPI 1878 CACGTG CAC^GTG
BbsI 1138 GTCTTC GAAGAC(2/6)
BpuAI 1138 GTCTTC GAAGAC(2/6)
BsaI 346 GAGACC GGTCTC(1/5)
BsmBI 202 GAGACG CGTCTC(1/5)
BspHI 836 TCATGA T^CATGA
BssSI 1129 CACGAG CACGAG(-5/-1)
BstAPI 456 GCAGGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 80 AGATCC [GA]^GATC[CT]
Bsu36I 765 CCTGAGG CC^T[ACGT]AGG
DrdI 1588 GACTCGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 765 CCTGAGG CC^T[ACGT]AGG
FspI 820 TGCGCA TGC^GCA
HincII 229 GTCAAC GT[CT]^[GA]AC
HindII 229 GTCAAC GT[CT]^[GA]AC
MflI 80 AGATCC [GA]^GATC[CT]
MluNI 1343 TGGCCA TGG^CCA
MscI 1343 TGGCCA TGG^CCA
NspI 1032 GCATGT [GA]CATG^[CT]
PflMI 1479 CCATCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1517 ACCGGT A^CCGGT
PmaCI 1878 CACGTG CAC^GTG
PmlI 1878 CACGTG CAC^GTG
PpuMI 602 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 836 TCATGA T^CATGA
SapI 570 GCTCTTC GCTCTTC(1/4)
SbfI 1752 CCTGCAGG CCTGCA^GG
ScaI 1046 AGTACT AGT^ACT
SfiI 744 GGCCGCTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 33 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 1752 CCTGCAGG CCTGCA^GG
StuI 731 AGGCCT AGG^CCT
TfiI 117 GATTC G^A[AT]TC
Van91I 1479 CCATCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1780 TCTAGA T^CTAGA
XhoII 80 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Alw44I 394 GTGCAC G^TGCAC
967 GTGCAC
Aor51HI 805 AGCGCT AGC^GCT
1334 AGCGCT
ApaLI 394 GTGCAC G^TGCAC
967 GTGCAC
BfaI 964 CTAG C^TAG
1781 CTAG
BlpI 219 GCTGAGC GC^T[ACGT]AGC
270 GCTCAGC
Bpu1102I 219 GCTGAGC GC^T[ACGT]AGC
270 GCTCAGC
BsaWI 971 ACCGGA [AT]^CCGG[AT]
1517 ACCGGT
BsgI 702 CTGCAC GTGCAG(16/14)
1267 CTGCAC
BsrBI 696 CCGCTC CCGCTC(-3/-3)
1053 CCGCTC
BssHII 538 GCGCGC G^CGCGC
685 GCGCGC
BstXI 350 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1684 CCATGCAGGTGG
CelII 219 GCTGAGC GC^T[ACGT]AGC
270 GCTCAGC
EarI 571 CTCTTC CTCTTC(1/4)
649 CTCTTC
Eco47III 805 AGCGCT AGC^GCT
1334 AGCGCT
Ksp632I 571 CTCTTC CTCTTC(1/4)
649 CTCTTC
KspI 49 CCGCGG CCGC^GG
1631 CCGCGG
MaeI 964 CTAG C^TAG
1781 CTAG
MslI 1685 CATGCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1874 CATCCACGTG
SacII 49 CCGCGG CCGC^GG
1631 CCGCGG
TaqI 632 TCGA T^CGA
1361 TCGA
TthHB8I 632 TCGA T^CGA
1361 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbeI BbvI
BcnI BglI BmyI BpmI BsaAI
BsaHI BsaJI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeII MaeIII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV PleI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
StyI TseI Tsp45I TspRI XcmI