##################################
# NO CUT ENZYMES (  199 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvI        
BcgI        BciVI       BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BseAI       BseRI       BsgI        BsiEI       
BsiWI       BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     Fnu4HI      FseI        
HaeII       HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        ItaI        KasI        KpnI        
Ksp632I     KspI        MaeI        MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NlaIV       NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TaqI        Tru9I       TseI        Tsp45I      Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AciI          288  CCGC                 CCGC(-3/-1)
AcsI          145  GAATTT               [GA]^AATT[CT]
AfaI          307  GTAC                 GT^AC
ApoI          145  GAATTT               [GA]^AATT[CT]
AspHI          87  GTGCTC               G[AT]GC[AT]^C
AvaII         333  GGACC                G^G[AT]CC
AviII           5  TGCGCA               TGC^GCA
BbvCI         317  CCTCAGC              CCTCAGC(-5/-2)
BclI          221  TGATCA               T^GATCA
BmyI           87  GTGCTC               G[AGT]GC[ACT]^C
BsaJI         375  CCTGGG               C^C[ACGT][ACGT]GG
BsaWI         104  TCCGGT               [AT]^CCGG[AT]
BsiHKAI        87  GTGCTC               G[AT]GC[AT]^C
Bsp1286I       87  GTGCTC               G[AGT]GC[ACT]^C
BsrI          291  CCAGT                ACTGG(1/-1)
BstF5I        284  CATCC                GGATG(2/0)
BstXI         165  CCACAGAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI            6  GCGC                 GCG^C
Cfr13I        333  GGACC                G^G[ACGT]CC
DdeI          318  CTCAG                C^T[ACGT]AG
DpnII         222  GATC                 ^GATC
FauI          287  CCCGC                CCCGC(4/6)
FbaI          221  TGATCA               T^GATCA
FokI          284  CATCC                GGATG(9/13)
FspI            5  TGCGCA               TGC^GCA
HaeIII         83  GGCC                 GG^CC
HapII         105  CCGG                 C^CGG
HhaI            6  GCGC                 GCG^C
HinP1I          6  GCGC                 G^CGC
HinfI         380  GATTC                G^A[ACGT]TC
HpaII         105  CCGG                 C^CGG
HphI          219  GGTGA                GGTGA(8/7)
MaeII         239  ACGT                 A^CGT
MaeIII        134  GTTAC                ^GT[ACGT]AC
MboI          222  GATC                 ^GATC
MboII         340  GAAGA                GAAGA(8/7)
MslI          114  CACTGTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        320  CAGCTG               C[AC]G^C[GT]G
MspI          105  CCGG                 C^CGG
MwoI           75  GCTTATTAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII         222  GATC                 ^GATC
PvuII         320  CAGCTG               CAG^CTG
RsaI          307  GTAC                 GT^AC
Sau3AI        222  GATC                 ^GATC
Sau96I        333  GGACC                G^G[ACGT]CC
SmlI           31  CTCAAG               C^T[CT][GA]AG
TfiI          380  GATTC                G^A[AT]TC
TspRI         114  CACTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (    4 enzymes) #
###################################

AluI          171  AGCT                 AG^CT
              321  AGCT                
Cac8I         200  GCAGGC               GC[ACGT]^[ACGT]GC
              322  GCTGGC              
SfaNI          25  GATGC                GCATC(5/9)
              326  GCATC               
Tsp509I       146  AATT                 ^AATT
              180  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    7 enzymes) #
#########################################

BssKI       BstNI       EcoRII      MnlI        MvaI        
NlaIII      ScrFI