Restriction map for
pF1KB9980
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaHI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspEI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII Cfr10I CpoI
DraII EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRV FauI FseI FspI HaeII
HgaI Hin1I HpaI KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspBI StuI SwaI
Tth111I Van91I XcmI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AflIII 846 ACATGT A^C[GA][CT]GT
AlwNI 1784 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 1580 GAGCAC G[AT]GC[AT]^C
AvaI 226 CTCGAG C^[CT]CG[GA]G
AvaII 1997 GGTCC G^G[AT]CC
BamHI 544 GGATCC G^GATCC
BclI 285 TGATCA T^GATCA
BmyI 1580 GAGCAC G[AGT]GC[ACT]^C
BpmI 1795 CTGGAG CTGGAG(16/14)
BsaWI 495 TCCGGT [AT]^CCGG[AT]
BsgI 1330 GTGCAG GTGCAG(16/14)
BsiHKAI 1580 GAGCAC G[AT]GC[AT]^C
BsmFI 2025 GTCCC GGGAC(10/14)
BsoBI 226 CTCGAG C^[CT]CG[GA]G
Bsp1286I 1580 GAGCAC G[AGT]GC[ACT]^C
BspDI 1489 ATCGAT AT^CGAT
BspLU11I 846 ACATGT A^CATGT
BspMI 220 ACCTGC ACCTGC(4/8)
BstAPI 409 GCACTTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 529 GCGC GCG^C
ClaI 1489 ATCGAT AT^CGAT
DrdI 1827 GACTGCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 319 CCATGG C^C[GA][CT]GG
EcoNI 1261 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1746 GAATTC G^AATTC
EcoT22I 886 ATGCAT ATGCA^T
FbaI 285 TGATCA T^GATCA
HapII 496 CCGG C^CGG
HhaI 529 GCGC GCG^C
HinP1I 529 GCGC G^CGC
HincII 295 GTCAAC GT[CT]^[GA]AC
HindII 295 GTCAAC GT[CT]^[GA]AC
HindIII 999 AAGCTT A^AGCTT
HpaII 496 CCGG C^CGG
MspA1I 775 CAGCTG C[AC]G^C[GT]G
MspI 496 CCGG C^CGG
NcoI 319 CCATGG C^CATGG
NsiI 886 ATGCAT ATGCA^T
NspI 846 ACATGT [GA]CATG^[CT]
PaeR7I 226 CTCGAG C^TCGAG
PstI 8 CTGCAG CTGCA^G
PvuII 775 CAGCTG CAG^CTG
SapI 1778 GAAGAGC GCTCTTC(1/4)
SpeI 2098 ACTAGT A^CTAGT
Tsp45I 1903 GTCAC ^GT[GC]AC
XbaI 1843 TCTAGA T^CTAGA
XhoI 226 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 1316 CCGC CCGC(-3/-1)
912 GCGG
BbsI 1480 GAAGAC GAAGAC(2/6)
1957 GAAGAC
BglII 1290 AGATCT A^GATCT
1588 AGATCT
BpuAI 1480 GAAGAC GAAGAC(2/6)
1957 GAAGAC
BsaBI 58 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2077 GATGATGATC
BsaI 603 GAGACC GGTCTC(1/5)
1766 GGTCTC
BsiYI 1261 CCTTCAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1808 CCCGTAGCAGG
BslI 1261 CCTTCAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1808 CCCGTAGCAGG
BspHI 186 TCATGA T^CATGA
384 TCATGA
DraI 37 TTTAAA TTT^AAA
1870 TTTAAA
DraIII 410 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
904 CACACAGTG
EcoT14I 319 CCATGG C^C[AT][AT]GG
419 CCTTGG
MamI 58 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2077 GATGATGATC
MslI 733 CATGAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1465 CATATTGATG
NlaIV 544 GGATCC GG[ACGT]^[ACGT]CC
728 GGGGCC
PshAI 487 GACATTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1786 GACACTGGTC
RcaI 186 TCATGA T^CATGA
384 TCATGA
SfcI 8 CTGCAG C^T[GA][CT]AG
1104 CTATAG
SmlI 201 CTCAAG C^T[CT][GA]AG
226 CTCGAG
SspI 42 AATATT AAT^ATT
242 AATATT
StyI 319 CCATGG C^C[AT][AT]GG
419 CCTTGG
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BfaI BsaJI BseRI BsmAI
BsrI BssKI BstF5I BstNI BstYI
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MvaI MwoI NdeII NlaIII PleI
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII