Restriction map for
pF1KE0687
##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbsI BbvCI BciVI BclI BfrI
BglII BlnI BpuAI BsaAI BsaHI
BsaI BsiEI BsiWI BsmI Bsp1407I
BspDI BspLU11I BsrBI BsrDI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstYI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI Hin1I
HindIII HpaI KasI KpnI KspI
MaeII MfeI MflI MluI MunI
NaeI NarI NcoI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PstI PvuI
RsrII SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI StuI StyI SwaI TspRI
Tth111I XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccII 576 CGCG CG^CG
AccIII 887 TCCGGA T^CCGGA
AcsI 721 AAATTT [GA]^AATT[CT]
ApoI 721 AAATTT [GA]^AATT[CT]
AseI 690 ATTAAT AT^TAAT
AsnI 690 ATTAAT AT^TAAT
AspHI 841 GAGCTC G[AT]GC[AT]^C
BcgI 938 GCATGCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1000 GCCTGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 159 GCTCAGC GC^T[ACGT]AGC
BpmI 838 CTGGAG CTGGAG(16/14)
Bpu1102I 159 GCTCAGC GC^T[ACGT]AGC
BsaBI 978 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 887 TCCGGA [AT]^CCGG[AT]
BseAI 887 TCCGGA T^CCGGA
BsgI 127 GTGCAG GTGCAG(16/14)
BsiHKAI 841 GAGCTC G[AT]GC[AT]^C
BsmBI 73 GAGACG CGTCTC(1/5)
BspEI 887 TCCGGA T^CCGGA
BspMI 809 ACCTGC ACCTGC(4/8)
BsrFI 859 ACCGGC [GA]^CCGG[CT]
BstEII 222 GGTCACC G^GT[ACGT]ACC
BstPI 222 GGTCACC G^GT[ACGT]ACC
BstUI 576 CGCG CG^CG
BstXI 908 CCAACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 159 GCTCAGC GC^T[ACGT]AGC
Cfr10I 859 ACCGGC [GA]^CCGG[CT]
DraII 823 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 823 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 222 GGTCACC G^GT[ACGT]ACC
MamI 978 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 887 TCCGGA T^CCGGA
MvnI 576 CGCG CG^CG
NheI 730 GCTAGC G^CTAGC
PleI 519 GACTC GAGTC(4/5)
PshBI 690 ATTAAT AT^TAAT
PvuII 850 CAGCTG CAG^CTG
SacI 841 GAGCTC GAGCT^C
SexAI 493 ACCTGGT A^CC[AT]GGT
SmaI 446 CCCGGG CCC^GGG
SmlI 310 CTCAAG C^T[CT][GA]AG
TaqI 65 TCGA T^CGA
Tsp45I 223 GTCAC ^GT[GC]AC
TthHB8I 65 TCGA T^CGA
XbaI 408 TCTAGA T^CTAGA
XcmI 350 CCACCTACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 446 CCCGGG C^CCGGG
XmaI 446 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AlwI 933 GATCC GGATC(4/5)
90 GGATC
BalI 917 TGGCCA TGG^CCA
1145 TGGCCA
BanII 61 GGGCTC G[GA]GC[CT]^C
841 GAGCTC
BcnI 446 CCCGG CC^[GC]GG
447 CCGGG
BsmAI 73 GAGAC GTCTC(1/5)
272 GTCTC
BspHI 515 TCATGA T^CATGA
1115 TCATGA
DdeI 160 CTCAG C^T[ACGT]AG
1041 CTCAG
DpnII 91 GATC ^GATC
933 GATC
EaeI 917 TGGCCA [CT]^GGCC[GA]
1145 TGGCCA
EarI 274 CTCTTC CTCTTC(1/4)
961 CTCTTC
FauI 1005 GCGGG CCCGC(4/6)
1217 GCGGG
HgaI 75 GACGC GACGC(5/10)
1091 GCGTC
HincII 10 GTTGAC GT[CT]^[GA]AC
1093 GTCAAC
HindII 10 GTTGAC GT[CT]^[GA]AC
1093 GTCAAC
Ksp632I 274 CTCTTC CTCTTC(1/4)
961 CTCTTC
MboI 91 GATC ^GATC
933 GATC
MluNI 917 TGGCCA TGG^CCA
1145 TGGCCA
MscI 917 TGGCCA TGG^CCA
1145 TGGCCA
MseI 389 TTAA T^TAA
691 TTAA
NciI 446 CCCGG CC^[GC]GG
447 CCGGG
NdeI 683 CATATG CA^TATG
1258 CATATG
NdeII 91 GATC ^GATC
933 GATC
NspI 281 GCATGC [GA]CATG^[CT]
938 GCATGC
PflMI 107 CCAAAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
995 CCAACGCCTGG
RcaI 515 TCATGA T^CATGA
1115 TCATGA
Sau3AI 91 GATC ^GATC
933 GATC
SphI 281 GCATGC GCATG^C
938 GCATGC
TfiI 132 GATTC G^A[AT]TC
198 GATTC
Tru9I 389 TTAA T^TAA
691 TTAA
Tsp509I 666 AATT ^AATT
722 AATT
Van91I 107 CCAAAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
995 CCAACGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AfaI AluI AvaI AvaII
BanI BbvI BfaI BmyI BsaJI
BseRI BsiYI BslI BsmFI BsoBI
Bsp1286I BssKI BstF5I BstNI Cac8I
CfoI Cfr13I EcoRII Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeIII MboII MnlI MslI MspA1I
MspI MvaI MwoI NlaIII NlaIV
RsaI Sau96I ScrFI SfaNI TseI