Restriction map for
pF1KB0434
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# NO CUT ENZYMES ( 114 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI AvaI BamHI BbeI
BbrPI BbvCI BciVI BclI BcnI
BlpI Bpu1102I BsaBI BsaHI BsaWI
BseAI BsgI BsiWI BsoBI Bsp1407I
BspDI BspEI BsrBI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I CelII ClaI CpoI DraIII
DrdI DsaI Eam1105I Eco47III EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
HaeII HgaI Hin1I HincII HindII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI MvnI
NarI NciI NcoI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SpeI Sse8387I
SspBI SwaI Tsp45I Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AlwNI 2570 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 939 CCTAAGG CC^T[ACGT]AGG
AseI 1218 ATTAAT AT^TAAT
AsnI 1218 ATTAAT AT^TAAT
AviII 435 TGCGCA TGC^GCA
AvrII 2439 CCTAGG C^CTAGG
BanI 873 GGTGCC G^G[CT][GA]CC
BanII 1802 GAGCTC G[GA]GC[CT]^C
BbsI 973 GTCTTC GAAGAC(2/6)
BcgI 705 GCAACTATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 876 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2439 CCTAGG C^CTAGG
BpmI 2395 CTCCAG CTGGAG(16/14)
BpuAI 973 GTCTTC GAAGAC(2/6)
BsaAI 1784 TACGTA [CT]AC^GT[GA]
BsaI 610 GGTCTC GGTCTC(1/5)
BseRI 1897 GAGGAG GAGGAG(10/8)
BsiEI 5 CGGCCG CG[GA][CT]^CG
BsmBI 2140 CGTCTC CGTCTC(1/5)
BsmI 30 GAATGC GAATGC(1/-1)
BssSI 1234 CTCGTG CACGAG(-5/-1)
BstXI 863 CCAGGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 939 CCTAAGG CC^T[ACGT]AGG
DraI 916 TTTAAA TTT^AAA
DraII 353 GGGACCT [GA]G^G[ACGT]CC[CT]
EagI 5 CGGCCG C^GGCCG
EclXI 5 CGGCCG C^GGCCG
Eco52I 5 CGGCCG C^GGCCG
Eco81I 939 CCTAAGG CC^T[ACGT]AGG
EcoO109I 353 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 2271 GAATTC G^AATTC
FauI 18 CCCGC CCCGC(4/6)
FspI 435 TGCGCA TGC^GCA
NaeI 3 GCCGGC GCC^GGC
NdeI 1610 CATATG CA^TATG
NgoMIV 3 GCCGGC G^CCGGC
PflMI 1302 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 353 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 1218 ATTAAT AT^TAAT
PvuII 2300 CAGCTG CAG^CTG
SacI 1802 GAGCTC GAGCT^C
ScaI 1130 AGTACT AGT^ACT
SnaBI 1784 TACGTA TAC^GTA
SphI 1257 GCATGC GCATG^C
SspI 1267 AATATT AAT^ATT
StuI 1304 AGGCCT AGG^CCT
Van91I 1302 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflII 348 CTTAAG C^TTAAG
1513 CTTAAG
AflIII 1757 ACATGT A^C[GA][CT]GT
2401 ACATGT
AlwI 2326 GATCC GGATC(4/5)
47 GGATC
AspHI 1392 GTGCTC G[AT]GC[AT]^C
1802 GAGCTC
BalI 1940 TGGCCA TGG^CCA
2585 TGGCCA
BfrI 348 CTTAAG C^TTAAG
1513 CTTAAG
BglII 384 AGATCT A^GATCT
1688 AGATCT
BsiHKAI 1392 GTGCTC G[AT]GC[AT]^C
1802 GAGCTC
BsmFI 353 GGGAC GGGAC(10/14)
867 GGGAC
BspLU11I 1757 ACATGT A^CATGT
2401 ACATGT
BspMI 432 ACCTGC ACCTGC(4/8)
607 GCAGGT
BsrDI 2282 CATTGC GCAATG(2/0)
1489 GCAATG
BsrFI 3 GCCGGC [GA]^CCGG[CT]
1067 GCCGGT
BstAPI 956 GCAAGGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1672 GCAAGAATTGC
CfoI 34 GCGC GCG^C
436 GCGC
Cfr10I 3 GCCGGC [GA]^CCGG[CT]
1067 GCCGGT
HapII 4 CCGG C^CGG
1068 CCGG
HhaI 34 GCGC GCG^C
436 GCGC
HinP1I 34 GCGC G^CGC
436 GCGC
HpaII 4 CCGG C^CGG
1068 CCGG
MaeII 251 ACGT A^CGT
1785 ACGT
MluNI 1940 TGGCCA TGG^CCA
2585 TGGCCA
MscI 1940 TGGCCA TGG^CCA
2585 TGGCCA
MslI 250 CACGTTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
317 CACTAAAATG
MspA1I 1421 CAGCGG C[AC]G^C[GT]G
2300 CAGCTG
MspI 4 CCGG C^CGG
1068 CCGG
PleI 2019 GACTC GAGTC(4/5)
234 GAGTC
SapI 1799 GAAGAGC GCTCTTC(1/4)
36 GCTCTTC
TaqI 429 TCGA T^CGA
1644 TCGA
TthHB8I 429 TCGA T^CGA
1644 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
Asp700I AvaII BbvI BfaI BmyI
BsaJI BsiYI BslI BsmAI Bsp1286I
BspHI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EaeI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HindIII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV NspI
PstI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp509I TspRI
XhoII XmnI