Restriction map for
pF1KB0471
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI Alw44I Aor51HI ApaLI AscI
Asp718I AspI AvaI AviII AvrII
BanI BbeI BbrPI BbsI BcgI
BciVI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BseAI BsgI BsiEI BsiWI
BsoBI Bsp1407I BspDI BspEI BsrBI
BsrFI BsrGI BssHII BstBI BstEII
BstPI BstUI CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
FseI FspI HgaI Hin1I HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PstI PvuI PvuII RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI Tth111I TthHB8I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AccI 207 GTATAC GT^[AC][GT]AC
AclI 351 AACGTT AA^CGTT
AfaI 184 GTAC GT^AC
AflII 813 CTTAAG C^TTAAG
AflIII 392 ACATGT A^C[GA][CT]GT
AlwNI 716 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1111 CCTGAGG CC^T[ACGT]AGG
ApaI 1177 GGGCCC GGGCC^C
AseI 442 ATTAAT AT^TAAT
AsnI 442 ATTAAT AT^TAAT
Asp700I 15 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1380 GGATCC G^GATCC
BbvCI 1153 CCTCAGC CCTCAGC(-5/-2)
BbvI 982 GCAGC GCAGC(8/12)
BclI 1488 TGATCA T^GATCA
BcnI 1083 CCCGG CC^[GC]GG
BfaI 328 CTAG C^TAG
BfrI 813 CTTAAG C^TTAAG
BpmI 1204 CTCCAG CTGGAG(16/14)
BsaWI 143 TCCGGT [AT]^CCGG[AT]
BsmBI 1349 CGTCTC CGTCTC(1/5)
BsmFI 1426 GGGAC GGGAC(10/14)
BsmI 15 GAATGC GAATGC(1/-1)
BspHI 215 TCATGA T^CATGA
BspLU11I 392 ACATGT A^CATGT
BsrI 1071 CCAGT ACTGG(1/-1)
Bst1107I 207 GTATAC GTA^TAC
BstAPI 715 GCAGAATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 1527 GGATG GGATG(2/0)
BstXI 893 CCATGGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1380 GGATCC [GA]^GATC[CT]
BstZ17I 207 GTATAC GTA^TAC
Bsu36I 1111 CCTGAGG CC^T[ACGT]AGG
CfoI 985 GCGC GCG^C
Eco81I 1111 CCTGAGG CC^T[ACGT]AGG
EcoRV 126 GATATC GAT^ATC
EcoT22I 1015 ATGCAT ATGCA^T
FbaI 1488 TGATCA T^GATCA
Fnu4HI 982 GCAGC GC^[ACGT]GC
FokI 1527 GGATG GGATG(9/13)
HaeII 984 AGCGCC [GA]GCGC^[CT]
HhaI 985 GCGC GCG^C
HinP1I 985 GCGC G^CGC
ItaI 982 GCAGC GC^[ACGT]GC
MaeI 328 CTAG C^TAG
MaeII 352 ACGT A^CGT
MflI 1380 GGATCC [GA]^GATC[CT]
NciI 1083 CCCGG CC^[GC]GG
NcoI 893 CCATGG C^CATGG
NdeI 83 CATATG CA^TATG
NsiI 1015 ATGCAT ATGCA^T
NspI 392 ACATGT [GA]CATG^[CT]
PflMI 1404 CCATTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1208 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 442 ATTAAT AT^TAAT
Psp1406I 351 AACGTT AA^CGTT
RcaI 215 TCATGA T^CATGA
RsaI 184 GTAC GT^AC
SacI 1126 GAGCTC GAGCT^C
TseI 982 GCAGC G^C[AT]GC
Van91I 1404 CCATTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 893 CCATGGATATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1380 GGATCC [GA]^GATC[CT]
XmnI 15 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AhdI 1022 GACTGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1081 GACCCGGAGTC
AluI 1127 AGCT AG^CT
1234 AGCT
AlwI 1381 GATCC GGATC(4/5)
1380 GGATC
AspEI 1022 GACTGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1081 GACCCGGAGTC
AspHI 637 GAGCAC G[AT]GC[AT]^C
1126 GAGCTC
BanII 1126 GAGCTC G[GA]GC[CT]^C
1177 GGGCCC
BsiHKAI 637 GAGCAC G[AT]GC[AT]^C
1126 GAGCTC
BsmAI 666 GTCTC GTCTC(1/5)
1350 GTCTC
BspMI 875 ACCTGC ACCTGC(4/8)
1106 ACCTGC
BsrDI 131 CATTGC GCAATG(2/0)
200 CATTGC
BssSI 307 CACGAG CACGAG(-5/-1)
67 CTCGTG
DsaI 893 CCATGG C^C[GA][CT]GG
1238 CCACGG
Eam1105I 1022 GACTGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1081 GACCCGGAGTC
EcoNI 1107 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1181 CCTGTGAGAGG
EcoT14I 893 CCATGG C^C[AT][AT]GG
1521 CCTTGG
FauI 341 GCGGG CCCGC(4/6)
1418 GCGGG
HapII 144 CCGG C^CGG
1084 CCGG
HincII 1369 GTTGAC GT[CT]^[GA]AC
1516 GTTGAC
HindII 1369 GTTGAC GT[CT]^[GA]AC
1516 GTTGAC
HpaII 144 CCGG C^CGG
1084 CCGG
MspA1I 339 CAGCGG C[AC]G^C[GT]G
1416 CAGCGG
MspI 144 CCGG C^CGG
1084 CCGG
MwoI 715 GCAGAATCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1101 GCCTGACCTGC
SfcI 1061 CTACAG C^T[GA][CT]AG
1230 CTACAG
SmlI 813 CTTAAG C^T[CT][GA]AG
1170 CTTGAG
StyI 893 CCATGG C^C[AT][AT]GG
1521 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AcsI ApoI AvaII BalI
BmyI BsaJI BseRI BsiYI BslI
Bsp1286I BssKI BstNI Cac8I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII HaeIII HinfI HphI
Ksp632I MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MvaI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI StuI TfiI
Tru9I Tsp45I Tsp509I TspRI