Restriction map for
pF1KB9989
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AgeI AhdI Alw44I AlwI AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AvaI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BciVI BclI
BcnI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsiEI BsiWI BsmI
BsoBI Bsp1407I BspDI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstEII
BstPI BstUI BstXI BstYI BstZ17I
CelII Cfr10I ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI FauI FbaI FseI
FspI HindIII HpaI KasI KpnI
KspI MaeII MfeI MflI MluI
MunI MvnI NaeI NarI NciI
NgoMIV NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI StuI
SwaI XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccIII 171 TCCGGA T^CCGGA
AcyI 868 GGCGTC G[GA]^CG[CT]C
AflII 1066 CTTAAG C^TTAAG
AocI 772 CCTCAGG CC^T[ACGT]AGG
AspI 1401 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 900 TGGCCA TGG^CCA
BcgI 157 GCAGTGGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1066 CTTAAG C^TTAAG
BpmI 203 CTGGAG CTGGAG(16/14)
BsaBI 430 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 868 GGCGTC G[GA]^CG[CT]C
BsaWI 171 TCCGGA [AT]^CCGG[AT]
BseAI 171 TCCGGA T^CCGGA
BseRI 706 GAGGAG GAGGAG(10/8)
BsmBI 143 CGTCTC CGTCTC(1/5)
BspEI 171 TCCGGA T^CCGGA
BspMI 752 GCAGGT ACCTGC(4/8)
BsrDI 1203 GCAATG GCAATG(2/0)
BssKI 700 CCTGG ^CC[ACGT]GG
BstBI 1214 TTCGAA TT^CGAA
BstNI 700 CCTGG CC^[AT]GG
Bsu36I 772 CCTCAGG CC^T[ACGT]AGG
CfoI 966 GCGC GCG^C
DpnII 1243 GATC ^GATC
DraII 754 AGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 217 GACCCTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 772 CCTCAGG CC^T[ACGT]AGG
EcoNI 1052 CCTATGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 754 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRII 700 CCTGG ^CC[AT]GG
HaeII 965 GGCGCT [GA]GCGC^[CT]
HapII 172 CCGG C^CGG
HgaI 869 GCGTC GACGC(5/10)
HhaI 966 GCGC GCG^C
Hin1I 868 GGCGTC G[GA]^CG[CT]C
HinP1I 966 GCGC G^CGC
HpaII 172 CCGG C^CGG
MamI 430 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 1243 GATC ^GATC
MluNI 900 TGGCCA TGG^CCA
MroI 171 TCCGGA T^CCGGA
MscI 900 TGGCCA TGG^CCA
MspA1I 88 CCGCTG C[AC]G^C[GT]G
MspI 172 CCGG C^CGG
MvaI 700 CCTGG CC^[AT]GG
NdeII 1243 GATC ^GATC
NheI 1425 GCTAGC G^CTAGC
NspV 1214 TTCGAA TT^CGAA
PflFI 1401 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1434 CCAATCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 754 AGGTCCT [GA]G^G[AT]CC[CT]
SapI 631 GAAGAGC GCTCTTC(1/4)
Sau3AI 1243 GATC ^GATC
ScrFI 700 CCTGG CC^[ACGT]GG
SfuI 1214 TTCGAA TT^CGAA
SmlI 1066 CTTAAG C^T[CT][GA]AG
SphI 1429 GCATGC GCATG^C
SspI 562 AATATT AAT^ATT
TfiI 55 GAATC G^A[AT]TC
Tth111I 1401 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1434 CCAATCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AfaI 1123 GTAC GT^AC
1362 GTAC
AflIII 723 ACATGT A^C[GA][CT]GT
1702 ACATGT
AspHI 1302 GAGCAC G[AT]GC[AT]^C
1466 GAGCAC
BbsI 711 GAAGAC GAAGAC(2/6)
1611 GAAGAC
BmyI 1302 GAGCAC G[AGT]GC[ACT]^C
1466 GAGCAC
BpuAI 711 GAAGAC GAAGAC(2/6)
1611 GAAGAC
BsaI 256 GAGACC GGTCTC(1/5)
1717 GGTCTC
BsiHKAI 1302 GAGCAC G[AT]GC[AT]^C
1466 GAGCAC
Bsp1286I 1302 GAGCAC G[AGT]GC[ACT]^C
1466 GAGCAC
BspHI 1644 TCATGA T^CATGA
1680 TCATGA
BspLU11I 723 ACATGT A^CATGT
1702 ACATGT
BstAPI 1383 GCACTCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1522 GCAGGCTATGC
DraI 541 TTTAAA TTT^AAA
1636 TTTAAA
DsaI 583 CCATGG C^C[GA][CT]GG
923 CCATGG
EaeI 23 TGGCCG [CT]^GGCC[GA]
900 TGGCCA
EarI 631 GAAGAG CTCTTC(1/4)
691 GAAGAG
EcoRV 1009 GATATC GAT^ATC
1342 GATATC
EcoT14I 583 CCATGG C^C[AT][AT]GG
923 CCATGG
EcoT22I 410 ATGCAT ATGCA^T
554 ATGCAT
HincII 798 GTCAAC GT[CT]^[GA]AC
1261 GTCAAC
HindII 798 GTCAAC GT[CT]^[GA]AC
1261 GTCAAC
HphI 210 TCACC GGTGA(8/7)
1549 TCACC
Ksp632I 631 GAAGAG CTCTTC(1/4)
691 GAAGAG
MaeIII 989 GTCAC ^GT[ACGT]AC
1399 GTGAC
MslI 355 CATATCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1675 CATCCTCATG
NcoI 583 CCATGG C^CATGG
923 CCATGG
NdeI 401 CATATG CA^TATG
1513 CATATG
NsiI 410 ATGCAT ATGCA^T
554 ATGCAT
PstI 1025 CTGCAG CTGCA^G
1394 CTGCAG
RcaI 1644 TCATGA T^CATGA
1680 TCATGA
RsaI 1123 GTAC GT^AC
1362 GTAC
StyI 583 CCATGG C^C[AT][AT]GG
923 CCATGG
TaqI 1215 TCGA T^CGA
1632 TCGA
Tsp45I 989 GTCAC ^GT[GC]AC
1399 GTGAC
TthHB8I 1215 TCGA T^CGA
1632 TCGA
XcmI 1268 CCATTGTTTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1411 CCAAATGGAAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AcsI AluI ApoI AvaII
BbvI BfaI BsaJI BsgI BsiYI
BslI BsmAI BsmFI BsrI BstF5I
Cac8I Cfr13I DdeI Fnu4HI FokI
HaeIII HinfI ItaI MaeI MboII
MnlI MseI MwoI NlaIII NlaIV
NspI PleI Sau96I SfaNI SfcI
Tru9I TseI Tsp509I TspRI