Restriction map for
pF1KB6622
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# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BglI
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmFI Bsp1286I BspDI
BspEI BsrBI BssHII BssKI BssSI
Bst1107I BstAPI BstBI BstNI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
ClaI CpoI DraII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoRII EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HaeIII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MunI MvaI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI ScrFI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI TfiI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AciI 828 GCGG CCGC(-3/-1)
AflII 138 CTTAAG C^TTAAG
AflIII 577 ACATGT A^C[GA][CT]GT
AlwI 208 GGATC GGATC(4/5)
AseI 1149 ATTAAT AT^TAAT
AsnI 1149 ATTAAT AT^TAAT
AvaI 203 CCCGAG C^[CT]CG[GA]G
AvaII 199 GGACC G^G[AT]CC
BfaI 446 CTAG C^TAG
BfrI 138 CTTAAG C^TTAAG
BglII 900 AGATCT A^GATCT
BsaAI 71 TACGTG [CT]AC^GT[GA]
BsgI 545 CTGCAC GTGCAG(16/14)
BsiYI 48 CCTTTCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 48 CCTTTCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 1236 GAATGC GAATGC(1/-1)
BsoBI 203 CCCGAG C^[CT]CG[GA]G
Bsp1407I 951 TGTACA T^GTACA
BspHI 1115 TCATGA T^CATGA
BspLU11I 577 ACATGT A^CATGT
BsrDI 991 GCAATG GCAATG(2/0)
BsrFI 635 ACCGGC [GA]^CCGG[CT]
BsrGI 951 TGTACA T^GTACA
BsrI 457 CCAGT ACTGG(1/-1)
BstEII 436 GGTGACC G^GT[ACGT]ACC
BstF5I 1289 CATCC GGATG(2/0)
BstPI 436 GGTGACC G^GT[ACGT]ACC
BstYI 900 AGATCT [GA]^GATC[CT]
Cfr10I 635 ACCGGC [GA]^CCGG[CT]
Cfr13I 199 GGACC G^G[ACGT]CC
DraIII 39 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1021 GAAGAG CTCTTC(1/4)
EcoNI 48 CCTTTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 436 GGTGACC G^GT[ACGT]ACC
EcoRI 654 GAATTC G^AATTC
FokI 1289 CATCC GGATG(9/13)
HapII 636 CCGG C^CGG
HpaII 636 CCGG C^CGG
Ksp632I 1021 GAAGAG CTCTTC(1/4)
MaeI 446 CTAG C^TAG
MaeII 72 ACGT A^CGT
MflI 900 AGATCT [GA]^GATC[CT]
MspA1I 1198 CAGCTG C[AC]G^C[GT]G
MspI 636 CCGG C^CGG
MwoI 843 GCTATTGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 199 GGACCC GG[ACGT]^[ACGT]CC
PshBI 1149 ATTAAT AT^TAAT
PvuII 1198 CAGCTG CAG^CTG
RcaI 1115 TCATGA T^CATGA
SapI 1021 GAAGAGC GCTCTTC(1/4)
Sau96I 199 GGACC G^G[ACGT]CC
ScaI 792 AGTACT AGT^ACT
SmlI 138 CTTAAG C^T[CT][GA]AG
SspBI 951 TGTACA T^GTACA
SwaI 1001 ATTTAAAT ATTT^AAAT
Tsp45I 437 GTGAC ^GT[GC]AC
XhoII 900 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Asp700I 751 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
801 GAAGCATTTC
BspMI 708 ACCTGC ACCTGC(4/8)
824 ACCTGC
Cac8I 852 GCTTGC GC[ACGT]^[ACGT]GC
913 GCAAGC
DsaI 168 CCATGG C^C[GA][CT]GG
1160 CCATGG
EcoT14I 168 CCATGG C^C[AT][AT]GG
1160 CCATGG
HindIII 390 AAGCTT A^AGCTT
422 AAGCTT
HinfI 93 GAGTC G^A[ACGT]TC
934 GACTC
HphI 436 GGTGA GGTGA(8/7)
633 TCACC
NcoI 168 CCATGG C^CATGG
1160 CCATGG
NspI 577 ACATGT [GA]CATG^[CT]
839 ACATGC
PleI 934 GACTC GAGTC(4/5)
93 GAGTC
SfaNI 849 GATGC GCATC(5/9)
874 GCATC
SspI 684 AATATT AAT^ATT
946 AATATT
StyI 168 CCATGG C^C[AT][AT]GG
1160 CCATGG
TaqI 864 TCGA T^CGA
937 TCGA
TspRI 458 CAGTG CA[GC]TG[ACGT][ACGT]^
1279 CAGTG
TthHB8I 864 TCGA T^CGA
937 TCGA
XmnI 751 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
801 GAAGCATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BsaJI DdeI DpnII DraI Fnu4HI
ItaI MaeIII MboI MboII MnlI
MseI MslI NdeII NlaIII PstI
RsaI Sau3AI SfcI Tru9I TseI
Tsp509I