##################################
# NO CUT ENZYMES ( 152 enzymes) #
##################################
Acc65I AccI AccII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BanII BbeI BbrPI
BbsI BbvCI BciVI BclI BcnI
BfaI BfrI BglI BlnI BpuAI
BsaBI BsaI BseRI BsgI BsiEI
BsiWI BsmI BspDI BspHI BspLU11I
BspMI BsrDI BsrFI BssHII BssKI
Bst1107I BstAPI BstBI BstEII BstNI
BstPI BstUI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRII EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MfeI MluI MspA1I
MunI MvaI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScrFI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tsp45I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 502 GACGTC GACGT^C
AccIII 47 TCCGGA T^CCGGA
AcsI 130 AAATTT [GA]^AATT[CT]
Aor51HI 704 AGCGCT AGC^GCT
ApoI 130 AAATTT [GA]^AATT[CT]
AvaII 112 GGTCC G^G[AT]CC
BamHI 44 GGATCC G^GATCC
BanI 780 GGTGCC G^G[CT][GA]CC
BcgI 53 GCAGACGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaWI 47 TCCGGA [AT]^CCGG[AT]
BseAI 47 TCCGGA T^CCGGA
BsmBI 641 GAGACG CGTCTC(1/5)
Bsp1407I 450 TGTACA T^GTACA
BspEI 47 TCCGGA T^CCGGA
BsrBI 754 GAGCGG CCGCTC(-3/-3)
BsrGI 450 TGTACA T^GTACA
BssSI 700 CACGAG CACGAG(-5/-1)
BstF5I 599 GGATG GGATG(2/0)
DsaI 776 CCATGG C^C[GA][CT]GG
Eco47III 704 AGCGCT AGC^GCT
FauI 756 GCGGG CCCGC(4/6)
FokI 599 GGATG GGATG(9/13)
HapII 48 CCGG C^CGG
HgaI 56 GACGC GACGC(5/10)
HpaII 48 CCGG C^CGG
HphI 613 GGTGA GGTGA(8/7)
MaeIII 175 GTTAC ^GT[ACGT]AC
MroI 47 TCCGGA T^CCGGA
MseI 14 TTAA T^TAA
MslI 774 CACCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 48 CCGG C^CGG
NcoI 776 CCATGG C^CATGG
NdeI 479 CATATG CA^TATG
NspI 231 ACATGC [GA]CATG^[CT]
PleI 518 GAGTC GAGTC(4/5)
ScaI 315 AGTACT AGT^ACT
SmlI 292 CTTGAG C^T[CT][GA]AG
SspBI 450 TGTACA T^GTACA
Tru9I 14 TTAA T^TAA
TspRI 384 CACTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 56 GACGCC G[GA]^CG[CT]C
502 GACGTC
AspHI 771 GAGCAC G[AT]GC[AT]^C
913 GAGCAC
AvaI 61 CTCGGG C^[CT]CG[GA]G
638 CCCGAG
BalI 380 TGGCCA TGG^CCA
683 TGGCCA
BglII 288 AGATCT A^GATCT
907 AGATCT
BlpI 540 GCTAAGC GC^T[ACGT]AGC
768 GCTGAGC
Bpu1102I 540 GCTAAGC GC^T[ACGT]AGC
768 GCTGAGC
BsaAI 37 TACGTG [CT]AC^GT[GA]
573 TACGTG
BsaHI 56 GACGCC G[GA]^CG[CT]C
502 GACGTC
BsiHKAI 771 GAGCAC G[AT]GC[AT]^C
913 GAGCAC
BsmFI 500 GGGAC GGGAC(10/14)
834 GGGAC
BsoBI 61 CTCGGG C^[CT]CG[GA]G
638 CCCGAG
CelII 540 GCTAAGC GC^T[ACGT]AGC
768 GCTGAGC
Cfr13I 112 GGTCC G^G[ACGT]CC
758 GGGCC
Hin1I 56 GACGCC G[GA]^CG[CT]C
502 GACGTC
HindIII 98 AAGCTT A^AGCTT
874 AAGCTT
MboII 195 GAAGA GAAGA(8/7)
744 GAAGA
MluNI 380 TGGCCA TGG^CCA
683 TGGCCA
MscI 380 TGGCCA TGG^CCA
683 TGGCCA
MwoI 676 GCTGCCTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
696 GCAGCACGAGC
NlaIV 44 GGATCC GG[ACGT]^[ACGT]CC
780 GGTGCC
Sau96I 112 GGTCC G^G[ACGT]CC
758 GGGCC
SfcI 712 CTACAG C^T[GA][CT]AG
903 CTACAG
Tsp509I 10 AATT ^AATT
131 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BmyI BpmI BsaJI BsiYI BslI
BsmAI Bsp1286I BsrI BstYI Cac8I
CfoI DdeI DpnII EaeI EcoT14I
Fnu4HI HaeII HaeIII HhaI HinP1I
HinfI ItaI MaeII MboI MflI
MnlI NdeII NlaIII RsaI Sau3AI
SfaNI StyI TaqI TfiI TseI
TthHB8I XhoII