Restriction map for
pF1KB6758
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# NO CUT ENZYMES ( 183 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AvaI AviII
AvrII BalI BamHI BanII BbeI
BbrPI BcgI BciVI BclI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstBI BstEII BstF5I
BstPI BstUI BstYI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FokI FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI HphI KasI KpnI
Ksp632I KspI MaeII MaeIII MamI
MfeI MflI MluI MluNI MroI
MscI MseI MslI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I Tsp45I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AciI 112 CCGC CCGC(-3/-1)
AspI 279 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 251 GGACC G^G[AT]CC
BanI 403 GGTGCC G^G[CT][GA]CC
BbsI 30 GAAGAC GAAGAC(2/6)
BbvCI 374 CCTCAGC CCTCAGC(-5/-2)
BbvI 189 GCAGC GCAGC(8/12)
BfaI 305 CTAG C^TAG
BlpI 114 GCTGAGC GC^T[ACGT]AGC
BmyI 404 GTGCCC G[AGT]GC[ACT]^C
BpmI 212 CTCCAG CTGGAG(16/14)
Bpu1102I 114 GCTGAGC GC^T[ACGT]AGC
BpuAI 30 GAAGAC GAAGAC(2/6)
BseRI 7 GAGGAG GAGGAG(10/8)
BsgI 85 GTGCAG GTGCAG(16/14)
BsmBI 277 GAGACG CGTCTC(1/5)
BsmFI 394 GGGAC GGGAC(10/14)
BsmI 308 GAATGC GAATGC(1/-1)
Bsp1286I 404 GTGCCC G[AGT]GC[ACT]^C
BstAPI 338 GCAAGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 384 CCAAGTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 114 GCTGAGC GC^T[ACGT]AGC
CfoI 318 GCGC GCG^C
Cfr13I 251 GGACC G^G[ACGT]CC
DsaI 80 CCGTGG C^C[GA][CT]GG
EaeI 62 TGGCCG [CT]^GGCC[GA]
EcoT14I 17 CCAAGG C^C[AT][AT]GG
HhaI 318 GCGC GCG^C
HinP1I 318 GCGC G^CGC
MaeI 305 CTAG C^TAG
MboII 30 GAAGA GAAGA(8/7)
MspA1I 112 CCGCTG C[AC]G^C[GT]G
NlaIII 236 CATG CATG^
PflFI 279 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 283 GAGTC GAGTC(4/5)
Sau96I 251 GGACC G^G[ACGT]CC
SfaNI 224 GCATC GCATC(5/9)
SfcI 398 CTATAG C^T[GA][CT]AG
StuI 131 AGGCCT AGG^CCT
StyI 17 CCAAGG C^C[AT][AT]GG
TaqI 182 TCGA T^CGA
TfiI 173 GATTC G^A[AT]TC
TseI 189 GCAGC G^C[AT]GC
Tsp509I 426 AATT ^AATT
Tth111I 279 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 182 TCGA T^CGA
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# TWO CUT ENZYMES ( 19 enzymes) #
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BcnI 65 CCGGG CC^[GC]GG
419 CCCGG
BstNI 254 CCTGG CC^[AT]GG
300 CCAGG
Cac8I 119 GCAAGC GC[ACGT]^[ACGT]GC
338 GCAAGC
DpnII 96 GATC ^GATC
168 GATC
EcoRII 254 CCTGG ^CC[AT]GG
300 CCAGG
Fnu4HI 111 GCCGC GC^[ACGT]GC
189 GCAGC
HaeIII 63 GGCC GG^CC
132 GGCC
HapII 65 CCGG C^CGG
420 CCGG
HinfI 173 GATTC G^A[ACGT]TC
283 GAGTC
HpaII 65 CCGG C^CGG
420 CCGG
ItaI 111 GCCGC GC^[ACGT]GC
189 GCAGC
MboI 96 GATC ^GATC
168 GATC
MspI 65 CCGG C^CGG
420 CCGG
MvaI 254 CCTGG CC^[AT]GG
300 CCAGG
NciI 65 CCGGG CC^[GC]GG
419 CCCGG
NdeII 96 GATC ^GATC
168 GATC
NlaIV 76 GGCTCC GG[ACGT]^[ACGT]CC
403 GGTGCC
Sau3AI 96 GATC ^GATC
168 GATC
TspRI 313 CAGTG CA[GC]TG[ACGT][ACGT]^
332 CACTG
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# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
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AluI BsaJI BsiYI BslI BsmAI
BssKI DdeI MnlI MwoI ScrFI