Restriction map for
pF1KB6161
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccIII AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BamHI BanII BbrPI BbvCI
BciVI BcnI BfrI BlnI BlpI
BmyI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI Bsp1286I Bsp1407I BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstXI BstZ17I CelII Cfr10I
CpoI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO109I
EcoO65I EcoRI EcoRV FauI FseI
FspI HapII HindIII HpaII KpnI
KspI MamI MluI MroI MspA1I
MspI NaeI NciI NcoI NdeI
NgoMIV NotI NruI NspI PacI
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI PstI
PvuI PvuII RcaI RsrII SacI
SacII SapI SbfI ScaI SexAI
SfiI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XcmI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccII 1591 CGCG CG^CG
AciI 1585 GCGG CCGC(-3/-1)
AclI 1514 AACGTT AA^CGTT
AcyI 202 GGCGCC G[GA]^CG[CT]C
AlwNI 1109 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 529 CCTAAGG CC^T[ACGT]AGG
AvaI 1502 CTCGAG C^[CT]CG[GA]G
AvaII 564 GGACC G^G[AT]CC
BalI 1571 TGGCCA TGG^CCA
BanI 202 GGCGCC G^G[CT][GA]CC
BbeI 202 GGCGCC GGCGC^C
BbsI 1181 GAAGAC GAAGAC(2/6)
BcgI 64 CGACAGGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1365 TGATCA T^GATCA
BglI 194 GCCCCTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 645 AGATCT A^GATCT
BpmI 1097 CTGGAG CTGGAG(16/14)
BpuAI 1181 GAAGAC GAAGAC(2/6)
BsaHI 202 GGCGCC G[GA]^CG[CT]C
BsgI 1375 GTGCAG GTGCAG(16/14)
BsoBI 1502 CTCGAG C^[CT]CG[GA]G
BspDI 254 ATCGAT AT^CGAT
BspMI 1434 ACCTGC ACCTGC(4/8)
BstBI 95 TTCGAA TT^CGAA
BstUI 1591 CGCG CG^CG
Bsu36I 529 CCTAAGG CC^T[ACGT]AGG
Cfr13I 564 GGACC G^G[ACGT]CC
ClaI 254 ATCGAT AT^CGAT
DsaI 441 CCGTGG C^C[GA][CT]GG
EaeI 1571 TGGCCA [CT]^GGCC[GA]
Eco81I 529 CCTAAGG CC^T[ACGT]AGG
EcoT14I 390 CCTTGG C^C[AT][AT]GG
FbaI 1365 TGATCA T^GATCA
HgaI 1592 GCGTC GACGC(5/10)
Hin1I 202 GGCGCC G[GA]^CG[CT]C
HpaI 12 GTTAAC GTT^AAC
KasI 202 GGCGCC G^GCGCC
MaeII 1515 ACGT A^CGT
MluNI 1571 TGGCCA TGG^CCA
MscI 1571 TGGCCA TGG^CCA
MslI 807 CATTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1591 CGCG CG^CG
NarI 202 GGCGCC GG^CGCC
NheI 1083 GCTAGC G^CTAGC
NlaIV 202 GGCGCC GG[ACGT]^[ACGT]CC
NspV 95 TTCGAA TT^CGAA
PaeR7I 1502 CTCGAG C^TCGAG
PflMI 385 CCAAACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 1514 AACGTT AA^CGTT
SalI 62 GTCGAC G^TCGAC
Sau96I 564 GGACC G^G[ACGT]CC
SfoI 202 GGCGCC GGC^GCC
SfuI 95 TTCGAA TT^CGAA
StyI 390 CCTTGG C^C[AT][AT]GG
TspRI 548 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 385 CCAAACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 477 TCTAGA T^CTAGA
XhoI 1502 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 62 GTCGAC GT^[AC][GT]AC
517 GTAGAC
BsaJI 390 CCTTGG C^C[ACGT][ACGT]GG
441 CCGTGG
BseRI 656 GAGGAG GAGGAG(10/8)
1202 GAGGAG
BsmAI 1003 GAGAC GTCTC(1/5)
1677 GTCTC
BsmFI 544 GGGAC GGGAC(10/14)
1250 GTCCC
BsmI 70 GAATGC GAATGC(1/-1)
1169 GAATGC
BssKI 304 CCAGG ^CC[ACGT]GG
1051 CCAGG
BstNI 304 CCAGG CC^[AT]GG
1051 CCAGG
CfoI 203 GCGC GCG^C
881 GCGC
EarI 1700 GAAGAG CTCTTC(1/4)
1767 GAAGAG
EcoNI 298 CCTCGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1192 CCTGATGAAGG
EcoRII 304 CCAGG ^CC[AT]GG
1051 CCAGG
EcoT22I 111 ATGCAT ATGCA^T
977 ATGCAT
HaeII 202 GGCGCC [GA]GCGC^[CT]
880 AGCGCC
HaeIII 1572 GGCC GG^CC
1721 GGCC
HhaI 203 GCGC GCG^C
881 GCGC
HinP1I 203 GCGC G^CGC
881 GCGC
Ksp632I 1700 GAAGAG CTCTTC(1/4)
1767 GAAGAG
MvaI 304 CCAGG CC^[AT]GG
1051 CCAGG
NsiI 111 ATGCAT ATGCA^T
977 ATGCAT
ScrFI 304 CCAGG CC^[ACGT]GG
1051 CCAGG
SmlI 963 CTTGAG C^T[CT][GA]AG
1502 CTCGAG
Tsp45I 895 GTCAC ^GT[GC]AC
1324 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BfaI BsiYI BslI BsrI
BstF5I BstYI Cac8I DdeI DpnII
DraI Fnu4HI FokI HincII HindII
HinfI HphI ItaI MaeI MaeIII
MboI MboII MfeI MflI MnlI
MseI MunI MwoI NdeII NlaIII
RsaI Sau3AI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp509I TthHB8I
XhoII