Restriction map for
pF1KE0785
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI Alw44I AlwI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BamHI BbrPI BbsI BciVI BclI
BfrI BglII BlnI BpuAI BsaAI
BsaBI BsaI BseAI BseRI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I ClaI DraI DrdI Eam1105I
EarI Eco47III EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
HindIII HpaI Ksp632I MamI MboII
MfeI MflI MluI MroI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
SacI SalI SapI SbfI ScaI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XhoII XmnI
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# ONE CUT ENZYMES ( 69 enzymes) #
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AcsI 553 AAATTC [GA]^AATT[CT]
AcyI 528 GGCGCC G[GA]^CG[CT]C
AflIII 203 ACGTGT A^C[GA][CT]GT
AocI 196 CCTCAGG CC^T[ACGT]AGG
ApoI 553 AAATTC [GA]^AATT[CT]
AspHI 211 GAGCAC G[AT]GC[AT]^C
BanII 761 GGGCTC G[GA]GC[CT]^C
BbeI 528 GGCGCC GGCGC^C
BbvCI 1033 GCTGAGG CCTCAGC(-5/-2)
BcgI 663 GCAGCGACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 931 CTAG C^TAG
BglI 583 GCCAAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 417 GCTGAGC GC^T[ACGT]AGC
BpmI 64 CTGGAG CTGGAG(16/14)
Bpu1102I 417 GCTGAGC GC^T[ACGT]AGC
BsaHI 528 GGCGCC G[GA]^CG[CT]C
BsaWI 891 TCCGGT [AT]^CCGG[AT]
BsiEI 370 CGGCCG CG[GA][CT]^CG
BsiHKAI 211 GAGCAC G[AT]GC[AT]^C
BsmAI 82 GAGAC GTCTC(1/5)
BsrFI 525 ACCGGC [GA]^CCGG[CT]
BsrI 723 CCAGT ACTGG(1/-1)
BssSI 388 CTCGTG CACGAG(-5/-1)
BstAPI 888 GCATCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 824 CCAAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 196 CCTCAGG CC^T[ACGT]AGG
CelII 417 GCTGAGC GC^T[ACGT]AGC
Cfr10I 525 ACCGGC [GA]^CCGG[CT]
CpoI 522 CGGACCG CG^G[AT]CCG
DpnII 540 GATC ^GATC
EagI 370 CGGCCG C^GGCCG
EclXI 370 CGGCCG C^GGCCG
Eco52I 370 CGGCCG C^GGCCG
Eco81I 196 CCTCAGG CC^T[ACGT]AGG
EcoNI 96 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 528 GGCGCC G[GA]^CG[CT]C
HincII 424 GTTGAC GT[CT]^[GA]AC
HindII 424 GTTGAC GT[CT]^[GA]AC
KasI 528 GGCGCC G^GCGCC
KspI 291 CCGCGG CCGC^GG
MaeI 931 CTAG C^TAG
MaeII 203 ACGT A^CGT
MaeIII 609 GTCAC ^GT[ACGT]AC
MboI 540 GATC ^GATC
MseI 1052 TTAA T^TAA
MslI 613 CACCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 528 GGCGCC GG^CGCC
NdeII 540 GATC ^GATC
PaeR7I 318 CTCGAG C^TCGAG
PflMI 234 CCAGCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1000 GAGTC GAGTC(4/5)
PvuII 164 CAGCTG CAG^CTG
RsrII 522 CGGACCG CG^G[AT]CCG
SacII 291 CCGCGG CCGC^GG
Sau3AI 540 GATC ^GATC
SexAI 869 ACCAGGT A^CC[AT]GGT
SfiI 582 GGCCAAAAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 528 GGCGCC GGC^GCC
SmaI 532 CCCGGG CCC^GGG
TaqI 319 TCGA T^CGA
Tru9I 1052 TTAA T^TAA
Tsp45I 609 GTCAC ^GT[GC]AC
Tsp509I 554 AATT ^AATT
TspRI 996 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 319 TCGA T^CGA
Van91I 234 CCAGCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 318 CTCGAG C^TCGAG
XmaCI 532 CCCGGG C^CCGGG
XmaI 532 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
Acc65I 866 GGTACC G^GTACC
1042 GGTACC
AlwNI 475 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
778 CAGTTGCTG
Asp718I 866 GGTACC G^GTACC
1042 GGTACC
BalI 581 TGGCCA TGG^CCA
956 TGGCCA
BsgI 352 CTGCAC GTGCAG(16/14)
100 GTGCAG
BssHII 329 GCGCGC G^CGCGC
455 GCGCGC
DraIII 822 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
992 CACTCAGTG
HaeII 60 GGCGCT [GA]GCGC^[CT]
528 GGCGCC
KpnI 866 GGTACC GGTAC^C
1042 GGTACC
MluNI 581 TGGCCA TGG^CCA
956 TGGCCA
MscI 581 TGGCCA TGG^CCA
956 TGGCCA
NcoI 578 CCATGG C^CATGG
1046 CCATGG
SmlI 318 CTCGAG C^T[CT][GA]AG
646 CTCAAG
XcmI 133 CCACCCCCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
584 CCAAAAAGGCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
AvaII BanI BbvI BcnI BmyI
BsaJI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV PpuMI PstI RsaI Sau96I
ScrFI SfaNI SfcI StyI TfiI
TseI