Restriction map for
pF1KB5894
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# NO CUT ENZYMES ( 129 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AvaI AviII BamHI
BanI BbeI BbrPI BbsI BcgI
BcnI BfrI BglI BglII BpuAI
BsaAI BsaHI BsaI BseRI BsgI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspHI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FauI FseI FspI HgaI Hin1I
HincII HindII HpaI KasI KpnI
KspI MflI MluI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccIII 949 TCCGGA T^CCGGA
AflIII 129 ACATGT A^C[GA][CT]GT
AlwI 1251 GATCC GGATC(4/5)
AseI 817 ATTAAT AT^TAAT
AsnI 817 ATTAAT AT^TAAT
Asp700I 1170 GAATAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1289 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1551 CCTAGG C^CTAGG
BalI 581 TGGCCA TGG^CCA
BanII 413 GAGCTC G[GA]GC[CT]^C
BbvCI 244 GCTGAGG CCTCAGC(-5/-2)
BciVI 301 GTATCC GTATCC(6/5)
BclI 89 TGATCA T^GATCA
BlnI 1551 CCTAGG C^CTAGG
BpmI 1409 CTGGAG CTGGAG(16/14)
BsaBI 1203 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 949 TCCGGA [AT]^CCGG[AT]
BseAI 949 TCCGGA T^CCGGA
BspEI 949 TCCGGA T^CCGGA
BspLU11I 129 ACATGT A^CATGT
BspMI 1333 ACCTGC ACCTGC(4/8)
BsrBI 545 CCGCTC CCGCTC(-3/-3)
BsrDI 1368 CATTGC GCAATG(2/0)
BstXI 232 CCAACCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1037 GCGC GCG^C
DraI 1444 TTTAAA TTT^AAA
DraII 22 GGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 581 TGGCCA [CT]^GGCC[GA]
EcoO109I 22 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 1492 GAATTC G^AATTC
FbaI 89 TGATCA T^GATCA
HaeII 1036 GGCGCT [GA]GCGC^[CT]
HapII 950 CCGG C^CGG
HhaI 1037 GCGC GCG^C
HinP1I 1037 GCGC G^CGC
HindIII 774 AAGCTT A^AGCTT
HpaII 950 CCGG C^CGG
MamI 1203 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1326 CAATTG C^AATTG
MluNI 581 TGGCCA TGG^CCA
MroI 949 TCCGGA T^CCGGA
MscI 581 TGGCCA TGG^CCA
MspI 950 CCGG C^CGG
MunI 1326 CAATTG C^AATTG
PflFI 1289 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 281 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 785 GACTC GAGTC(4/5)
PpuMI 22 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 817 ATTAAT AT^TAAT
PstI 1487 CTGCAG CTGCA^G
SacI 413 GAGCTC GAGCT^C
SapI 879 GCTCTTC GCTCTTC(1/4)
StuI 507 AGGCCT AGG^CCT
TaqI 850 TCGA T^CGA
Tth111I 1289 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 850 TCGA T^CGA
Van91I 281 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1497 CCAAAAGAAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1170 GAATAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 545 CCGC CCGC(-3/-1)
1372 GCGG
AfaI 188 GTAC GT^AC
1365 GTAC
AlwNI 694 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1562 CAGAGACTG
BfaI 913 CTAG C^TAG
1552 CTAG
BlpI 937 GCTCAGC GC^T[ACGT]AGC
1511 GCTCAGC
Bpu1102I 937 GCTCAGC GC^T[ACGT]AGC
1511 GCTCAGC
BsiYI 281 CCATGCTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
545 CCGCTCTCAGG
BslI 281 CCATGCTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
545 CCGCTCTCAGG
BsmI 596 GCATTC GAATGC(1/-1)
1318 GCATTC
BstAPI 654 GCACATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1309 GCAATAGCTGC
Cac8I 579 GCTGGC GC[ACGT]^[ACGT]GC
681 GCGAGC
CelII 937 GCTCAGC GC^T[ACGT]AGC
1511 GCTCAGC
EcoT14I 238 CCTTGG C^C[AT][AT]GG
1551 CCTAGG
MaeI 913 CTAG C^TAG
1552 CTAG
MaeII 1095 ACGT A^CGT
1293 ACGT
MseI 818 TTAA T^TAA
1445 TTAA
MspA1I 263 CAGCTG C[AC]G^C[GT]G
577 CAGCTG
NspI 129 ACATGT [GA]CATG^[CT]
930 GCATGT
PvuII 263 CAGCTG CAG^CTG
577 CAGCTG
RsaI 188 GTAC GT^AC
1365 GTAC
StyI 238 CCTTGG C^C[AT][AT]GG
1551 CCTAGG
TfiI 371 GAATC G^A[AT]TC
953 GAATC
Tru9I 818 TTAA T^TAA
1445 TTAA
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AcsI AluI ApoI AspHI AvaII
BbvI BmyI BsaJI BsiHKAI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MslI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI SfcI TseI Tsp45I
Tsp509I TspRI