Restriction map for
pF1KB7771
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspI
AvaI AvaII AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmFI BsmI BsoBI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BssHII
Bst1107I BstAPI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I Cac8I CelII
CfoI Cfr10I CpoI DraII DraIII
DrdI EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MslI MspA1I MunI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspI PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SbfI
ScaI SexAI SfcI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AflIII 186 ACGTGT A^C[GA][CT]GT
AhdI 1066 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 6 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1066 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1309 GAGCAC G[AT]GC[AT]^C
BanII 302 GAGCCC G[GA]GC[CT]^C
BcnI 984 CCGGG CC^[GC]GG
BpmI 691 CTGGAG CTGGAG(16/14)
BsaI 271 GAGACC GGTCTC(1/5)
BsiHKAI 1309 GAGCAC G[AT]GC[AT]^C
Bsp1407I 1301 TGTACA T^GTACA
BspDI 489 ATCGAT AT^CGAT
BspHI 954 TCATGA T^CATGA
BsrGI 1301 TGTACA T^GTACA
BstBI 368 TTCGAA TT^CGAA
BstYI 618 AGATCC [GA]^GATC[CT]
Cfr13I 484 GGGCC G^G[ACGT]CC
ClaI 489 ATCGAT AT^CGAT
DraI 636 TTTAAA TTT^AAA
Eam1105I 1066 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HincII 409 GTCAAC GT[CT]^[GA]AC
HindII 409 GTCAAC GT[CT]^[GA]AC
HindIII 949 AAGCTT A^AGCTT
MaeII 186 ACGT A^CGT
MflI 618 AGATCC [GA]^GATC[CT]
NciI 984 CCGGG CC^[GC]GG
NcoI 1269 CCATGG C^CATGG
NspV 368 TTCGAA TT^CGAA
RcaI 954 TCATGA T^CATGA
Sau96I 484 GGGCC G^G[ACGT]CC
SfuI 368 TTCGAA TT^CGAA
SspBI 1301 TGTACA T^GTACA
XhoII 618 AGATCC [GA]^GATC[CT]
XmnI 6 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 572 CCGC CCGC(-3/-1)
514 GCGG
AcsI 283 AAATTT [GA]^AATT[CT]
631 GAATTT
AlwI 580 GATCC GGATC(4/5)
619 GATCC
ApoI 283 AAATTT [GA]^AATT[CT]
631 GAATTT
BbvI 129 GCAGC GCAGC(8/12)
1130 GCAGC
BfaI 968 CTAG C^TAG
1031 CTAG
BsrI 555 ACTGG ACTGG(1/-1)
690 ACTGG
BssSI 308 CACGAG CACGAG(-5/-1)
1021 CACGAG
BstF5I 43 CATCC GGATG(2/0)
77 GGATG
DsaI 1140 CCACGG C^C[GA][CT]GG
1269 CCATGG
EcoT14I 995 CCAAGG C^C[AT][AT]GG
1269 CCATGG
Fnu4HI 129 GCAGC GC^[ACGT]GC
1130 GCAGC
FokI 43 CATCC GGATG(9/13)
77 GGATG
HaeIII 199 GGCC GG^CC
485 GGCC
ItaI 129 GCAGC GC^[ACGT]GC
1130 GCAGC
MaeI 968 CTAG C^TAG
1031 CTAG
NdeI 98 CATATG CA^TATG
155 CATATG
SapI 738 GAAGAGC GCTCTTC(1/4)
1237 GAAGAGC
SfaNI 126 GATGC GCATC(5/9)
492 GATGC
StyI 995 CCAAGG C^C[AT][AT]GG
1269 CCATGG
TseI 129 GCAGC G^C[AT]GC
1130 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AfaI AluI BmyI BsaJI BseRI
BsiYI BslI BsmAI Bsp1286I BssKI
BstNI DdeI DpnII EarI EcoRII
HapII HinfI HpaII HphI Ksp632I
MaeIII MboI MboII MnlI MseI
MspI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI ScrFI TaqI
TfiI Tru9I Tsp45I Tsp509I TspRI
TthHB8I