################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII Acc65I AccI AclI AflII AgeI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AvaI AvrII BalI BamHI BbrPI BcgI BciVI BfaI BfrI BglI BlnI BsaAI BsiEI BsiWI BsmBI BsoBI BspDI BsrDI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI HindIII HpaI KpnI KspI MaeI MaeII MfeI MluI MluNI MscI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StyI SwaI Tru9I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AcyI 361 GGCGCC G[GA]^CG[CT]C AfaI 160 GTAC GT^AC AhdI 172 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 172 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbeI 361 GGCGCC GGCGC^C BbvCI 339 GCTGAGG CCTCAGC(-5/-2) BsaBI 561 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 361 GGCGCC G[GA]^CG[CT]C BsmI 1249 GAATGC GAATGC(1/-1) Bsp1407I 159 TGTACA T^GTACA BspHI 281 TCATGA T^CATGA BsrBI 429 GAGCGG CCGCTC(-3/-3) BsrFI 1022 GCCGGC [GA]^CCGG[CT] BsrGI 159 TGTACA T^GTACA BstAPI 653 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr10I 1022 GCCGGC [GA]^CCGG[CT] DraIII 1192 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 467 CCGTGG C^C[GA][CT]GG EaeI 1020 TGGCCG [CT]^GGCC[GA] Eam1105I 172 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1159 CTCTTC CTCTTC(1/4) HaeII 361 GGCGCC [GA]GCGC^[CT] HgaI 583 GACGC GACGC(5/10) Hin1I 361 GGCGCC G[GA]^CG[CT]C KasI 361 GGCGCC G^GCGCC Ksp632I 1159 CTCTTC CTCTTC(1/4) MaeIII 7 GTCAC ^GT[ACGT]AC MamI 561 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 1022 GCCGGC GCC^GGC NarI 361 GGCGCC GG^CGCC NgoMIV 1022 GCCGGC G^CCGGC RcaI 281 TCATGA T^CATGA RsaI 160 GTAC GT^AC SacI 1312 GAGCTC GAGCT^C SfoI 361 GGCGCC GGC^GCC SspBI 159 TGTACA T^GTACA StuI 401 AGGCCT AGG^CCT TfiI 977 GATTC G^A[AT]TC Tsp45I 7 GTCAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccII 996 CGCG CG^CG 1195 CGCG AccIII 353 TCCGGA T^CCGGA 722 TCCGGA AcsI 627 GAATTT [GA]^AATT[CT] 1090 AAATTC ApoI 627 GAATTT [GA]^AATT[CT] 1090 AAATTC AviII 413 TGCGCA TGC^GCA 1352 TGCGCA BanI 344 GGTGCC G^G[CT][GA]CC 361 GGCGCC BanII 1078 GAGCCC G[GA]GC[CT]^C 1312 GAGCTC BbsI 381 GAAGAC GAAGAC(2/6) 1042 GAAGAC BlpI 648 GCTTAGC GC^T[ACGT]AGC 1330 GCTCAGC Bpu1102I 648 GCTTAGC GC^T[ACGT]AGC 1330 GCTCAGC BpuAI 381 GAAGAC GAAGAC(2/6) 1042 GAAGAC BsaI 126 GAGACC GGTCTC(1/5) 747 GGTCTC BsaWI 353 TCCGGA [AT]^CCGG[AT] 722 TCCGGA BseAI 353 TCCGGA T^CCGGA 722 TCCGGA BsgI 61 CTGCAC GTGCAG(16/14) 535 GTGCAG BspEI 353 TCCGGA T^CCGGA 722 TCCGGA BspMI 48 ACCTGC ACCTGC(4/8) 1051 GCAGGT BstUI 996 CGCG CG^CG 1195 CGCG CelII 648 GCTTAGC GC^T[ACGT]AGC 1330 GCTCAGC FspI 413 TGCGCA TGC^GCA 1352 TGCGCA MroI 353 TCCGGA T^CCGGA 722 TCCGGA MslI 271 CACTTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1296 CATGGCTATG MvnI 996 CGCG CG^CG 1195 CGCG PpuMI 34 GGGTCCC [GA]G^G[AT]CC[CT] 225 AGGTCCC ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AciI AflIII AluI AlwNI AspHI AvaII BbvI BclI BcnI BglII BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspLU11I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoNI EcoO109I EcoRII FauI FbaI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI Tsp509I TspRI TthHB8I XhoII