Restriction map for pF1KB7714
##################################
# NO CUT ENZYMES (  119 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AgeI        Alw44I      AlwI        AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspI        AvaI        AvrII       
BalI        BamHI       BbrPI       BcgI        BciVI       
BfaI        BfrI        BglI        BlnI        BsaAI       
BsiEI       BsiWI       BsmBI       BsoBI       BspDI       
BsrDI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        DrdI        EagI        
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FseI        
HindIII     HpaI        KpnI        KspI        MaeI        
MaeII       MfeI        MluI        MluNI       MscI        
MseI        MunI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspI        StyI        SwaI        
Tru9I       Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AcyI          361  GGCGCC               G[GA]^CG[CT]C
AfaI          160  GTAC                 GT^AC
AhdI          172  GACACAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         172  GACACAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI          361  GGCGCC               GGCGC^C
BbvCI         339  GCTGAGG              CCTCAGC(-5/-2)
BsaBI         561  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         361  GGCGCC               G[GA]^CG[CT]C
BsmI         1249  GAATGC               GAATGC(1/-1)
Bsp1407I      159  TGTACA               T^GTACA
BspHI         281  TCATGA               T^CATGA
BsrBI         429  GAGCGG               CCGCTC(-3/-3)
BsrFI        1022  GCCGGC               [GA]^CCGG[CT]
BsrGI         159  TGTACA               T^GTACA
BstAPI        653  GCAGTGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I       1022  GCCGGC               [GA]^CCGG[CT]
DraIII       1192  CACCGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          467  CCGTGG               C^C[GA][CT]GG
EaeI         1020  TGGCCG               [CT]^GGCC[GA]
Eam1105I      172  GACACAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1159  CTCTTC               CTCTTC(1/4)
HaeII         361  GGCGCC               [GA]GCGC^[CT]
HgaI          583  GACGC                GACGC(5/10)
Hin1I         361  GGCGCC               G[GA]^CG[CT]C
KasI          361  GGCGCC               G^GCGCC
Ksp632I      1159  CTCTTC               CTCTTC(1/4)
MaeIII          7  GTCAC                ^GT[ACGT]AC
MamI          561  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI         1022  GCCGGC               GCC^GGC
NarI          361  GGCGCC               GG^CGCC
NgoMIV       1022  GCCGGC               G^CCGGC
RcaI          281  TCATGA               T^CATGA
RsaI          160  GTAC                 GT^AC
SacI         1312  GAGCTC               GAGCT^C
SfoI          361  GGCGCC               GGC^GCC
SspBI         159  TGTACA               T^GTACA
StuI          401  AGGCCT               AGG^CCT
TfiI          977  GATTC                G^A[AT]TC
Tsp45I          7  GTCAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccII         996  CGCG                 CG^CG
             1195  CGCG                
AccIII        353  TCCGGA               T^CCGGA
              722  TCCGGA              
AcsI          627  GAATTT               [GA]^AATT[CT]
             1090  AAATTC              
ApoI          627  GAATTT               [GA]^AATT[CT]
             1090  AAATTC              
AviII         413  TGCGCA               TGC^GCA
             1352  TGCGCA              
BanI          344  GGTGCC               G^G[CT][GA]CC
              361  GGCGCC              
BanII        1078  GAGCCC               G[GA]GC[CT]^C
             1312  GAGCTC              
BbsI          381  GAAGAC               GAAGAC(2/6)
             1042  GAAGAC              
BlpI          648  GCTTAGC              GC^T[ACGT]AGC
             1330  GCTCAGC             
Bpu1102I      648  GCTTAGC              GC^T[ACGT]AGC
             1330  GCTCAGC             
BpuAI         381  GAAGAC               GAAGAC(2/6)
             1042  GAAGAC              
BsaI          126  GAGACC               GGTCTC(1/5)
              747  GGTCTC              
BsaWI         353  TCCGGA               [AT]^CCGG[AT]
              722  TCCGGA              
BseAI         353  TCCGGA               T^CCGGA
              722  TCCGGA              
BsgI           61  CTGCAC               GTGCAG(16/14)
              535  GTGCAG              
BspEI         353  TCCGGA               T^CCGGA
              722  TCCGGA              
BspMI          48  ACCTGC               ACCTGC(4/8)
             1051  GCAGGT              
BstUI         996  CGCG                 CG^CG
             1195  CGCG                
CelII         648  GCTTAGC              GC^T[ACGT]AGC
             1330  GCTCAGC             
FspI          413  TGCGCA               TGC^GCA
             1352  TGCGCA              
MroI          353  TCCGGA               T^CCGGA
              722  TCCGGA              
MslI          271  CACTTCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1296  CATGGCTATG          
MvnI          996  CGCG                 CG^CG
             1195  CGCG                
PpuMI          34  GGGTCCC              [GA]G^G[AT]CC[CT]
              225  AGGTCCC             


#########################################
# 3 OR MORE CUT ENZYMES (   76 enzymes) #
#########################################

AciI        AflIII      AluI        AlwNI       AspHI       
AvaII       BbvI        BclI        BcnI        BglII       
BmyI        BpmI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspLU11I    BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       EcoNI       EcoO109I    EcoRII      
FauI        FbaI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        MboI        
MboII       MflI        MnlI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PleI        PstI        PvuII       
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
TaqI        TseI        Tsp509I     TspRI       TthHB8I     
XhoII       
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