##################################
# NO CUT ENZYMES (  183 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfaI        BfrI        BglI        
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaHI       BsaWI       BseAI       BseRI       
BsiEI       BsiWI       BsiYI       BslI        BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspLU11I    BsrBI       BsrFI       BsrGI       BssHII      
BstAPI      BstBI       BstEII      BstPI       BstUI       
Bsu36I      CelII       CfoI        Cfr10I      Cfr13I      
ClaI        CpoI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HaeIII      HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MaeI        MaeII       MfeI        
MluI        MluNI       MroI        MscI        MunI        
MvnI        MwoI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        RsaI        RsrII       SacI        
SacII       SalI        Sau96I      SbfI        ScaI        
SexAI       SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccI          212  GTATAC               GT^[AC][GT]AC
AciI            7  CCGC                 CCGC(-3/-1)
AcsI          154  AAATTT               [GA]^AATT[CT]
AlwNI         351  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          154  AAATTT               [GA]^AATT[CT]
AspHI         137  GTGCTC               G[AT]GC[AT]^C
BamHI         526  GGATCC               G^GATCC
BcnI          530  CCGGG                CC^[GC]GG
BmyI          137  GTGCTC               G[AGT]GC[ACT]^C
BsaBI         623  GATCTGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          515  GAGACC               GGTCTC(1/5)
BsaJI         530  CCGGGG               C^C[ACGT][ACGT]GG
BsiHKAI       137  GTGCTC               G[AT]GC[AT]^C
Bsp1286I      137  GTGCTC               G[AGT]GC[ACT]^C
BspHI         567  TCATGA               T^CATGA
BspMI         382  ACCTGC               ACCTGC(4/8)
BsrDI         291  GCAATG               GCAATG(2/0)
BsrI           11  CCAGT                ACTGG(1/-1)
BssSI         104  CACGAG               CACGAG(-5/-1)
Bst1107I      212  GTATAC               GTA^TAC
BstF5I        316  CATCC                GGATG(2/0)
BstNI         145  CCAGG                CC^[AT]GG
BstXI         288  CCAGCAATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       212  GTATAC               GTA^TAC
DraI          157  TTTAAA               TTT^AAA
EcoRII        145  CCAGG                ^CC[AT]GG
FokI          316  CATCC                GGATG(9/13)
HapII         530  CCGG                 C^CGG
HpaII         530  CCGG                 C^CGG
MaeIII        102  GTCAC                ^GT[ACGT]AC
MamI          623  GATCTGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI          415  CACTGCTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        351  CAGCTG               C[AC]G^C[GT]G
MspI          530  CCGG                 C^CGG
MvaI          145  CCAGG                CC^[AT]GG
NciI          530  CCGGG                CC^[GC]GG
NlaIV         526  GGATCC               GG[ACGT]^[ACGT]CC
PvuII         351  CAGCTG               CAG^CTG
RcaI          567  TCATGA               T^CATGA
TfiI          610  GATTC                G^A[AT]TC
Tsp45I        102  GTCAC                ^GT[GC]AC
TspRI         415  CACTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

BglII         436  AGATCT               A^GATCT
              622  AGATCT              
BsgI          234  CTGCAC               GTGCAG(16/14)
              590  CTGCAC              
BsmAI         515  GAGAC                GTCTC(1/5)
              458  GTCTC               
BssKI         145  CCAGG                ^CC[ACGT]GG
              530  CCGGG               
DdeI          119  CTCAG                C^T[ACGT]AG
              520  CTAAG               
HphI          305  TCACC                GGTGA(8/7)
              487  TCACC               
NlaIII         89  CATG                 CATG^
              568  CATG                
ScrFI         145  CCAGG                CC^[ACGT]GG
              530  CCGGG               


#########################################
# 3 OR MORE CUT ENZYMES (   25 enzymes) #
#########################################

AluI        AlwI        BbvI        BsmFI       BstYI       
Cac8I       DpnII       EarI        Fnu4HI      HinfI       
ItaI        Ksp632I     MboI        MboII       MflI        
MnlI        MseI        NdeII       PleI        SapI        
Sau3AI      Tru9I       TseI        Tsp509I     XhoII