Restriction map for pF1KE0993
##################################
# NO CUT ENZYMES (  126 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AgeI        Alw44I      Aor51HI     ApaI        ApaLI       
AscI        Asp718I     AspHI       AspI        AvaI        
AviII       AvrII       BanII       BbeI        BbrPI       
BcgI        BcnI        BglI        BlnI        BlpI        
Bpu1102I    BsaBI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsoBI       BspDI       BspEI       BspHI       BsrBI       
BsrDI       BsrFI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstZ17I     
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraII       DraIII      DrdI        EagI        EclXI       
Eco47III    Eco52I      EcoNI       EcoO109I    EcoO65I     
EcoRI       FauI        FseI        FspI        HaeII       
HapII       HhaI        HinP1I      HindIII     HpaI        
HpaII       KasI        KpnI        KspI        MamI        
MluI        MroI        MspI        NaeI        NarI        
NciI        NdeI        NgoMIV      NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspI        StuI        
SwaI        Tth111I     Van91I      XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccII        1813  CGCG                 CG^CG
AcyI         1017  GACGCC               G[GA]^CG[CT]C
AflII         178  CTTAAG               C^TTAAG
AflIII       1652  ACATGT               A^C[GA][CT]GT
AseI          952  ATTAAT               AT^TAAT
AsnI          952  ATTAAT               AT^TAAT
Asp700I      1226  GAAGACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI         1833  GGTGCC               G^G[CT][GA]CC
BbvCI        1038  GCTGAGG              CCTCAGC(-5/-2)
BclI         1895  TGATCA               T^GATCA
BfrI          178  CTTAAG               C^TTAAG
BmyI          992  GGGCAC               G[AGT]GC[ACT]^C
BsaAI          85  TACGTG               [CT]AC^GT[GA]
BsaHI        1017  GACGCC               G[GA]^CG[CT]C
Bsp1286I      992  GGGCAC               G[AGT]GC[ACT]^C
Bsp1407I      219  TGTACA               T^GTACA
BspLU11I     1652  ACATGT               A^CATGT
BsrGI         219  TGTACA               T^GTACA
BstUI        1813  CGCG                 CG^CG
BstXI        1583  CCAGGTGTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
FbaI         1895  TGATCA               T^GATCA
HgaI         1017  GACGC                GACGC(5/10)
Hin1I        1017  GACGCC               G[GA]^CG[CT]C
HincII        436  GTTGAC               GT[CT]^[GA]AC
HindII        436  GTTGAC               GT[CT]^[GA]AC
MaeII          86  ACGT                 A^CGT
MfeI         1361  CAATTG               C^AATTG
MunI         1361  CAATTG               C^AATTG
MvnI         1813  CGCG                 CG^CG
NheI         1795  GCTAGC               G^CTAGC
PshBI         952  ATTAAT               AT^TAAT
PstI          979  CTGCAG               CTGCA^G
SapI         1882  GAAGAGC              GCTCTTC(1/4)
SphI          655  GCATGC               GCATG^C
SspBI         219  TGTACA               T^GTACA
XbaI         1617  TCTAGA               T^CTAGA
XmnI         1226  GAAGACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AocI          601  CCTGAGG              CC^T[ACGT]AGG
             1219  CCTGAGG             
BciVI         458  GGATAC               GTATCC(6/5)
             1390  GGATAC              
BfaI         1618  CTAG                 C^TAG
             1796  CTAG                
BglII         342  AGATCT               A^GATCT
             1135  AGATCT              
BpmI         1048  CTCCAG               CTGGAG(16/14)
             1921  CTCCAG              
BsmAI         470  GAGAC                GTCTC(1/5)
             1320  GTCTC               
BsmFI        1738  GGGAC                GGGAC(10/14)
             1656  GTCCC               
BspMI         977  ACCTGC               ACCTGC(4/8)
             1310  ACCTGC              
BsrI          577  ACTGG                ACTGG(1/-1)
              651  ACTGG               
Bsu36I        601  CCTGAGG              CC^T[ACGT]AGG
             1219  CCTGAGG             
DsaI         1337  CCATGG               C^C[GA][CT]GG
             1640  CCATGG              
Eco81I        601  CCTGAGG              CC^T[ACGT]AGG
             1219  CCTGAGG             
EcoRV         764  GATATC               GAT^ATC
             1567  GATATC              
MaeI         1618  CTAG                 C^TAG
             1796  CTAG                
MspA1I        421  CAGCTG               C[AC]G^C[GT]G
             1153  CAGCTG              
MwoI         1038  GCTGAGGAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1795  GCTAGCGAAGC         
NcoI         1337  CCATGG               C^CATGG
             1640  CCATGG              
PvuII         421  CAGCTG               CAG^CTG
             1153  CAGCTG              
ScaI          326  AGTACT               AGT^ACT
             1430  AGTACT              
SfcI          871  CTACAG               C^T[GA][CT]AG
              979  CTGCAG              
XcmI         1631  CCATCACCACCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1724  CCATGCAGAAGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   74 enzymes) #
#########################################

AciI        AcsI        AfaI        AhdI        AluI        
AlwI        AlwNI       ApoI        AspEI       AvaII       
BalI        BamHI       BbsI        BbvI        BpuAI       
BsaJI       BseRI       BsiYI       BslI        BsmI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DraI        EaeI        
Eam1105I    EarI        EcoRII      EcoT14I     EcoT22I     
Fnu4HI      FokI        HaeIII      HinfI       HphI        
ItaI        Ksp632I     MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MvaI        NdeII       NlaIII      NlaIV       
NsiI        NspI        PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       
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