################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AgeI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspHI AspI AvaI AviII AvrII BanII BbeI BbrPI BcgI BcnI BglI BlnI BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsoBI BspDI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI EagI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI FauI FseI FspI HaeII HapII HhaI HinP1I HindIII HpaI HpaII KasI KpnI KspI MamI MluI MroI MspI NaeI NarI NciI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI StuI SwaI Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccII 1813 CGCG CG^CG AcyI 1017 GACGCC G[GA]^CG[CT]C AflII 178 CTTAAG C^TTAAG AflIII 1652 ACATGT A^C[GA][CT]GT AseI 952 ATTAAT AT^TAAT AsnI 952 ATTAAT AT^TAAT Asp700I 1226 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanI 1833 GGTGCC G^G[CT][GA]CC BbvCI 1038 GCTGAGG CCTCAGC(-5/-2) BclI 1895 TGATCA T^GATCA BfrI 178 CTTAAG C^TTAAG BmyI 992 GGGCAC G[AGT]GC[ACT]^C BsaAI 85 TACGTG [CT]AC^GT[GA] BsaHI 1017 GACGCC G[GA]^CG[CT]C Bsp1286I 992 GGGCAC G[AGT]GC[ACT]^C Bsp1407I 219 TGTACA T^GTACA BspLU11I 1652 ACATGT A^CATGT BsrGI 219 TGTACA T^GTACA BstUI 1813 CGCG CG^CG BstXI 1583 CCAGGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG FbaI 1895 TGATCA T^GATCA HgaI 1017 GACGC GACGC(5/10) Hin1I 1017 GACGCC G[GA]^CG[CT]C HincII 436 GTTGAC GT[CT]^[GA]AC HindII 436 GTTGAC GT[CT]^[GA]AC MaeII 86 ACGT A^CGT MfeI 1361 CAATTG C^AATTG MunI 1361 CAATTG C^AATTG MvnI 1813 CGCG CG^CG NheI 1795 GCTAGC G^CTAGC PshBI 952 ATTAAT AT^TAAT PstI 979 CTGCAG CTGCA^G SapI 1882 GAAGAGC GCTCTTC(1/4) SphI 655 GCATGC GCATG^C SspBI 219 TGTACA T^GTACA XbaI 1617 TCTAGA T^CTAGA XmnI 1226 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AocI 601 CCTGAGG CC^T[ACGT]AGG 1219 CCTGAGG BciVI 458 GGATAC GTATCC(6/5) 1390 GGATAC BfaI 1618 CTAG C^TAG 1796 CTAG BglII 342 AGATCT A^GATCT 1135 AGATCT BpmI 1048 CTCCAG CTGGAG(16/14) 1921 CTCCAG BsmAI 470 GAGAC GTCTC(1/5) 1320 GTCTC BsmFI 1738 GGGAC GGGAC(10/14) 1656 GTCCC BspMI 977 ACCTGC ACCTGC(4/8) 1310 ACCTGC BsrI 577 ACTGG ACTGG(1/-1) 651 ACTGG Bsu36I 601 CCTGAGG CC^T[ACGT]AGG 1219 CCTGAGG DsaI 1337 CCATGG C^C[GA][CT]GG 1640 CCATGG Eco81I 601 CCTGAGG CC^T[ACGT]AGG 1219 CCTGAGG EcoRV 764 GATATC GAT^ATC 1567 GATATC MaeI 1618 CTAG C^TAG 1796 CTAG MspA1I 421 CAGCTG C[AC]G^C[GT]G 1153 CAGCTG MwoI 1038 GCTGAGGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1795 GCTAGCGAAGC NcoI 1337 CCATGG C^CATGG 1640 CCATGG PvuII 421 CAGCTG CAG^CTG 1153 CAGCTG ScaI 326 AGTACT AGT^ACT 1430 AGTACT SfcI 871 CTACAG C^T[GA][CT]AG 979 CTGCAG XcmI 1631 CCATCACCACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1724 CCATGCAGAAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 74 enzymes) # ######################################### AciI AcsI AfaI AhdI AluI AlwI AlwNI ApoI AspEI AvaII BalI BamHI BbsI BbvI BpuAI BsaJI BseRI BsiYI BslI BsmI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI EaeI Eam1105I EarI EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MvaI NdeII NlaIII NlaIV NsiI NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII