Restriction map for
pF1KB0407
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AccI AccIII AclI AflII AflIII
Aor51HI ApaI AscI AseI AsnI
Asp700I AspI AviII AvrII BalI
BamHI BbeI BbsI BcgI BciVI
BclI BfrI BglII BlnI BpuAI
BsaBI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstYI BstZ17I CfoI
ClaI CpoI DrdI EaeI EagI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HhaI HinP1I HincII
HindII HindIII HpaI KasI KspI
MamI MfeI MflI MluI MluNI
MroI MscI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SspBI SspI SwaI
Tth111I XbaI XcmI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AatII 727 GACGTC GACGT^C
Acc65I 941 GGTACC G^GTACC
AcsI 619 GAATTT [GA]^AATT[CT]
AcyI 727 GACGTC G[GA]^CG[CT]C
AgeI 938 ACCGGT A^CCGGT
AhdI 528 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 720 GTGCAC G^TGCAC
AlwI 183 GGATC GGATC(4/5)
AlwNI 498 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1148 CCTCAGG CC^T[ACGT]AGG
ApaLI 720 GTGCAC G^TGCAC
ApoI 619 GAATTT [GA]^AATT[CT]
Asp718I 941 GGTACC G^GTACC
AspEI 528 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 941 GGTACC G^G[CT][GA]CC
BbrPI 769 CACGTG CAC^GTG
BfaI 611 CTAG C^TAG
BglI 1192 GCCGAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 91 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 91 GCTAAGC GC^T[ACGT]AGC
BsaHI 727 GACGTC G[GA]^CG[CT]C
BsaI 213 GAGACC GGTCTC(1/5)
BseRI 961 GAGGAG GAGGAG(10/8)
BsrDI 1064 GCAATG GCAATG(2/0)
BsrFI 938 ACCGGT [GA]^CCGG[CT]
Bsu36I 1148 CCTCAGG CC^T[ACGT]AGG
CelII 91 GCTAAGC GC^T[ACGT]AGC
Cfr10I 938 ACCGGT [GA]^CCGG[CT]
DraI 628 TTTAAA TTT^AAA
DraII 453 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 202 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 528 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 925 GAAGAG CTCTTC(1/4)
Eco81I 1148 CCTCAGG CC^T[ACGT]AGG
EcoO109I 453 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 30 CCATGG C^C[AT][AT]GG
HgaI 1364 GCGTC GACGC(5/10)
Hin1I 727 GACGTC G[GA]^CG[CT]C
KpnI 941 GGTACC GGTAC^C
Ksp632I 925 GAAGAG CTCTTC(1/4)
MaeI 611 CTAG C^TAG
MslI 1293 CATGCACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 30 CCATGG C^CATGG
NspI 1292 GCATGC [GA]CATG^[CT]
PaeR7I 1327 CTCGAG C^TCGAG
PflMI 394 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 938 ACCGGT A^CCGGT
PmaCI 769 CACGTG CAC^GTG
PmlI 769 CACGTG CAC^GTG
PvuII 398 CAGCTG CAG^CTG
SacI 898 GAGCTC GAGCT^C
SapI 925 GAAGAGC GCTCTTC(1/4)
SbfI 503 CCTGCAGG CCTGCA^GG
SfaNI 65 GCATC GCATC(5/9)
SpeI 610 ACTAGT A^CTAGT
SphI 1292 GCATGC GCATG^C
Sse8387I 503 CCTGCAGG CCTGCA^GG
StuI 1166 AGGCCT AGG^CCT
StyI 30 CCATGG C^C[AT][AT]GG
Van91I 394 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1327 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 270 CGCG CG^CG
1363 CGCG
AvaI 308 CTCGGG C^[CT]CG[GA]G
1327 CTCGAG
BbvCI 318 CCTCAGC CCTCAGC(-5/-2)
147 GCTGAGG
BcnI 475 CCGGG CC^[GC]GG
1095 CCGGG
BpmI 24 CTCCAG CTGGAG(16/14)
248 CTGGAG
BsaAI 717 TACGTG [CT]AC^GT[GA]
769 CACGTG
BsaWI 569 ACCGGA [AT]^CCGG[AT]
938 ACCGGT
BsmAI 213 GAGAC GTCTC(1/5)
341 GAGAC
BsmI 240 GAATGC GAATGC(1/-1)
583 GAATGC
BsoBI 308 CTCGGG C^[CT]CG[GA]G
1327 CTCGAG
BsrBI 286 GAGCGG CCGCTC(-3/-3)
1013 GAGCGG
BstAPI 497 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
713 GCATTACGTGC
BstF5I 549 GGATG GGATG(2/0)
572 GGATG
BstUI 270 CGCG CG^CG
1363 CGCG
BstXI 538 CCATCTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
945 CCAGCAGTATGG
DsaI 30 CCATGG C^C[GA][CT]GG
217 CCGTGG
FokI 549 GGATG GGATG(9/13)
572 GGATG
MboII 925 GAAGA GAAGA(8/7)
68 TCTTC
MspA1I 398 CAGCTG C[AC]G^C[GT]G
1257 CCGCTG
MvnI 270 CGCG CG^CG
1363 CGCG
NciI 475 CCGGG CC^[GC]GG
1095 CCGGG
PstI 504 CTGCAG CTGCA^G
973 CTGCAG
SmlI 76 CTCAAG C^T[CT][GA]AG
1327 CTCGAG
TfiI 52 GATTC G^A[AT]TC
915 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BanII BbvI BmyI BsaJI BsgI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BspMI BsrI BssKI BstNI Cac8I
Cfr13I DdeI DpnII EcoRII FauI
Fnu4HI HaeIII HapII HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MnlI MseI MspI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfcI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I