Restriction map for
pF1KB0994
##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI AhdI AocI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AvaI AvrII BamHI
BbeI BbsI BcgI BciVI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaI BseAI BsiEI
BsiWI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FseI Hin1I HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MroI MseI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SapI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SpeI SphI SspBI SspI SwaI
TfiI Tru9I Tsp509I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 844 GTCTAC GT^[AC][GT]AC
Alw44I 520 GTGCAC G^TGCAC
Aor51HI 1346 AGCGCT AGC^GCT
ApaLI 520 GTGCAC G^TGCAC
AspI 280 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 599 TGCGCA TGC^GCA
BalI 234 TGGCCA TGG^CCA
BanI 266 GGTGCC G^G[CT][GA]CC
BbrPI 37 CACGTG CAC^GTG
BclI 464 TGATCA T^GATCA
BfaI 253 CTAG C^TAG
BglI 1117 GCCCTCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 875 TCCGGT [AT]^CCGG[AT]
BsmBI 87 CGTCTC CGTCTC(1/5)
BsmI 216 GAATGC GAATGC(1/-1)
DrdI 307 GACTGCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1346 AGCGCT AGC^GCT
EcoNI 1071 CCTGTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 464 TGATCA T^GATCA
FspI 599 TGCGCA TGC^GCA
HgaI 86 GCGTC GACGC(5/10)
HinfI 940 GAGTC G^A[ACGT]TC
MaeI 253 CTAG C^TAG
MluNI 234 TGGCCA TGG^CCA
MscI 234 TGGCCA TGG^CCA
NspI 962 ACATGC [GA]CATG^[CT]
PflFI 280 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 940 GAGTC GAGTC(4/5)
PmaCI 37 CACGTG CAC^GTG
PmlI 37 CACGTG CAC^GTG
PstI 781 CTGCAG CTGCA^G
SacI 1336 GAGCTC GAGCT^C
SbfI 780 CCTGCAGG CCTGCA^GG
SmlI 1174 CTCAAG C^T[CT][GA]AG
SnaBI 1186 TACGTA TAC^GTA
Sse8387I 780 CCTGCAGG CCTGCA^GG
StuI 564 AGGCCT AGG^CCT
Tth111I 280 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 85 CGCG CG^CG
595 CGCG
AflIII 38 ACGTGT A^C[GA][CT]GT
204 ACGTGT
AlwI 330 GATCC GGATC(4/5)
699 GATCC
AlwNI 242 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
784 CAGGAGCTG
AspHI 520 GTGCAC G[AT]GC[AT]^C
1336 GAGCTC
BbvCI 1126 GCTGAGG CCTCAGC(-5/-2)
1209 GCTGAGG
BsaAI 37 CACGTG [CT]AC^GT[GA]
1186 TACGTA
BsiHKAI 520 GTGCAC G[AT]GC[AT]^C
1336 GAGCTC
BsrDI 288 CATTGC GCAATG(2/0)
1041 CATTGC
BsrFI 5 GCCGGC [GA]^CCGG[CT]
51 GCCGGC
BssSI 652 CACGAG CACGAG(-5/-1)
1021 CACGAG
BstUI 85 CGCG CG^CG
595 CGCG
BstXI 36 CCACGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
392 CCAAGCGTGTGG
BstYI 329 AGATCC [GA]^GATC[CT]
698 AGATCC
Cfr10I 5 GCCGGC [GA]^CCGG[CT]
51 GCCGGC
EcoT14I 56 CCAAGG C^C[AT][AT]GG
935 CCAAGG
HaeII 741 GGCGCT [GA]GCGC^[CT]
1346 AGCGCT
HincII 124 GTCAAC GT[CT]^[GA]AC
1216 GTCAAC
HindII 124 GTCAAC GT[CT]^[GA]AC
1216 GTCAAC
MboII 76 GAAGA GAAGA(8/7)
696 GAAGA
MflI 329 AGATCC [GA]^GATC[CT]
698 AGATCC
MvnI 85 CGCG CG^CG
595 CGCG
NaeI 5 GCCGGC GCC^GGC
51 GCCGGC
NgoMIV 5 GCCGGC G^CCGGC
51 GCCGGC
PpuMI 658 GGGACCC [GA]G^G[AT]CC[CT]
1082 AGGACCT
SfcI 537 CTACAG C^T[GA][CT]AG
781 CTGCAG
StyI 56 CCAAGG C^C[AT][AT]GG
935 CCAAGG
TaqI 608 TCGA T^CGA
1059 TCGA
TthHB8I 608 TCGA T^CGA
1059 TCGA
XcmI 497 CCATGCTGACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
968 CCAAACGTGGCCTGG
XhoII 329 AGATCC [GA]^GATC[CT]
698 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI ApaI AvaII
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrBI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeII
MaeIII MboI MnlI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI TseI Tsp45I TspRI