##################################
# NO CUT ENZYMES ( 146 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaI BseAI BsiEI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspEI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I CelII CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MflI MluI MroI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 328 GACGTC GACGT^C
AcyI 328 GACGTC G[GA]^CG[CT]C
AflIII 707 ACACGT A^C[GA][CT]GT
AgeI 512 ACCGGT A^CCGGT
AlwI 577 GGATC GGATC(4/5)
AseI 970 ATTAAT AT^TAAT
AsnI 970 ATTAAT AT^TAAT
AspHI 1106 GAGCAC G[AT]GC[AT]^C
BalI 702 TGGCCA TGG^CCA
BanII 189 GGGCTC G[GA]GC[CT]^C
BsaBI 578 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 328 GACGTC G[GA]^CG[CT]C
BsaWI 512 ACCGGT [AT]^CCGG[AT]
BsiHKAI 1106 GAGCAC G[AT]GC[AT]^C
BspDI 547 ATCGAT AT^CGAT
BstXI 167 CCAACGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 547 ATCGAT AT^CGAT
DdeI 258 CTTAG C^T[ACGT]AG
DraI 210 TTTAAA TTT^AAA
DraIII 713 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 702 TGGCCA [CT]^GGCC[GA]
EarI 247 CTCTTC CTCTTC(1/4)
Hin1I 328 GACGTC G[GA]^CG[CT]C
Ksp632I 247 CTCTTC CTCTTC(1/4)
MamI 578 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 960 CAATTG C^AATTG
MluNI 702 TGGCCA TGG^CCA
MscI 702 TGGCCA TGG^CCA
MslI 1134 CATGAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 696 CAGCGG C[AC]G^C[GT]G
MunI 960 CAATTG C^AATTG
NdeI 1093 CATATG CA^TATG
NspI 81 GCATGC [GA]CATG^[CT]
PinAI 512 ACCGGT A^CCGGT
PshBI 970 ATTAAT AT^TAAT
SphI 81 GCATGC GCATG^C
TaqI 548 TCGA T^CGA
TthHB8I 548 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 304 CGCG CG^CG
385 CGCG
BcnI 310 CCGGG CC^[GC]GG
977 CCCGG
BmyI 189 GGGCTC G[AGT]GC[ACT]^C
1106 GAGCAC
BseRI 128 CTCCTC GAGGAG(10/8)
192 CTCCTC
BsgI 133 CTGCAC GTGCAG(16/14)
991 GTGCAG
Bsp1286I 189 GGGCTC G[AGT]GC[ACT]^C
1106 GAGCAC
BspHI 648 TCATGA T^CATGA
857 TCATGA
BsrFI 102 GCCGGT [GA]^CCGG[CT]
512 ACCGGT
BsrI 420 ACTGG ACTGG(1/-1)
1008 CCAGT
BstUI 304 CGCG CG^CG
385 CGCG
CfoI 4 GCGC GCG^C
386 GCGC
Cfr10I 102 GCCGGT [GA]^CCGG[CT]
512 ACCGGT
EcoT14I 173 CCATGG C^C[AT][AT]GG
1061 CCATGG
HgaI 205 GACGC GACGC(5/10)
813 GCGTC
HhaI 4 GCGC GCG^C
386 GCGC
HinP1I 4 GCGC G^CGC
386 GCGC
MaeII 329 ACGT A^CGT
709 ACGT
MvnI 304 CGCG CG^CG
385 CGCG
NciI 310 CCGGG CC^[GC]GG
977 CCCGG
NcoI 173 CCATGG C^CATGG
1061 CCATGG
PleI 938 GACTC GAGTC(4/5)
343 GAGTC
PpuMI 74 GGGACCC [GA]G^G[AT]CC[CT]
1113 AGGACCT
RcaI 648 TCATGA T^CATGA
857 TCATGA
StyI 173 CCATGG C^C[AT][AT]GG
1061 CCATGG
TfiI 381 GATTC G^A[AT]TC
435 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BsaJI BsiYI BslI BsmFI BssKI
BstF5I BstNI Cac8I Cfr13I DpnII
DraII DsaI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeIII MboI
MboII MnlI MseI MspI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI Tru9I
TseI Tsp45I Tsp509I TspRI