Restriction map for
pF1KB0469
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII AccI AclI AflII AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaLI AscI Asp700I AspEI AspHI
AspI AviII AvrII BalI BamHI
BbrPI BcgI BciVI BfrI BglI
BlnI BsaAI BsaBI BsiHKAI BsiWI
BsmI Bsp1407I BspDI BspLU11I BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstXI BstZ17I Cfr10I ClaI
CpoI DraIII DrdI Eam1105I Eco47III
EcoNI EcoT22I FseI FspI HgaI
HindIII HpaI KspI MamI MfeI
MluI MluNI MscI MslI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XhoI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 420 GGTACC G^GTACC
AccII 69 CGCG CG^CG
AccIII 1535 TCCGGA T^CCGGA
AcyI 43 GGCGCC G[GA]^CG[CT]C
AseI 1117 ATTAAT AT^TAAT
AsnI 1117 ATTAAT AT^TAAT
Asp718I 420 GGTACC G^GTACC
BbeI 43 GGCGCC GGCGC^C
BglII 1034 AGATCT A^GATCT
BlpI 1323 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1323 GCTGAGC GC^T[ACGT]AGC
BsaHI 43 GGCGCC G[GA]^CG[CT]C
BsaWI 1535 TCCGGA [AT]^CCGG[AT]
BseAI 1535 TCCGGA T^CCGGA
BsiEI 820 CGGCCG CG[GA][CT]^CG
BspEI 1535 TCCGGA T^CCGGA
BspHI 482 TCATGA T^CATGA
BssSI 994 CACGAG CACGAG(-5/-1)
BstEII 1411 GGTAACC G^GT[ACGT]ACC
BstPI 1411 GGTAACC G^GT[ACGT]ACC
BstUI 69 CGCG CG^CG
CelII 1323 GCTGAGC GC^T[ACGT]AGC
DraI 883 TTTAAA TTT^AAA
DsaI 224 CCATGG C^C[GA][CT]GG
EaeI 820 CGGCCG [CT]^GGCC[GA]
EagI 820 CGGCCG C^GGCCG
EarI 699 CTCTTC CTCTTC(1/4)
EclXI 820 CGGCCG C^GGCCG
Eco52I 820 CGGCCG C^GGCCG
EcoO65I 1411 GGTAACC G^GT[ACGT]ACC
EcoRI 1212 GAATTC G^AATTC
EcoRV 499 GATATC GAT^ATC
Hin1I 43 GGCGCC G[GA]^CG[CT]C
HincII 1135 GTTGAC GT[CT]^[GA]AC
HindII 1135 GTTGAC GT[CT]^[GA]AC
KasI 43 GGCGCC G^GCGCC
KpnI 420 GGTACC GGTAC^C
Ksp632I 699 CTCTTC CTCTTC(1/4)
MroI 1535 TCCGGA T^CCGGA
MvnI 69 CGCG CG^CG
NarI 43 GGCGCC GG^CGCC
NcoI 224 CCATGG C^CATGG
PflMI 21 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1117 ATTAAT AT^TAAT
PstI 1027 CTGCAG CTGCA^G
PvuII 1297 CAGCTG CAG^CTG
RcaI 482 TCATGA T^CATGA
SexAI 180 ACCAGGT A^CC[AT]GGT
SfcI 1027 CTGCAG C^T[GA][CT]AG
SfoI 43 GGCGCC GGC^GCC
SmlI 1387 CTCAAG C^T[CT][GA]AG
Tsp45I 1016 GTGAC ^GT[GC]AC
Van91I 21 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1257 TCTAGA T^CTAGA
XcmI 792 CCAGGAGACCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
ApaI 84 GGGCCC GGGCC^C
120 GGGCCC
AvaI 8 CCCGGG C^[CT]CG[GA]G
87 CCCGGG
BanI 43 GGCGCC G^G[CT][GA]CC
420 GGTACC
BbsI 1200 GAAGAC GAAGAC(2/6)
1302 GAAGAC
BbvCI 313 CCTCAGC CCTCAGC(-5/-2)
1072 CCTCAGC
BclI 941 TGATCA T^GATCA
1146 TGATCA
BpmI 982 CTCCAG CTGGAG(16/14)
646 CTGGAG
BpuAI 1200 GAAGAC GAAGAC(2/6)
1302 GAAGAC
BseRI 1420 GAGGAG GAGGAG(10/8)
1423 GAGGAG
BsgI 1377 GTGCAG GTGCAG(16/14)
1495 GTGCAG
BsmBI 827 GAGACG CGTCTC(1/5)
1503 GAGACG
BsoBI 8 CCCGGG C^[CT]CG[GA]G
87 CCCGGG
FbaI 941 TGATCA T^GATCA
1146 TGATCA
HaeII 43 GGCGCC [GA]GCGC^[CT]
1427 AGCGCC
HphI 720 GGTGA GGTGA(8/7)
813 GGTGA
MaeIII 1016 GTGAC ^GT[ACGT]AC
1412 GTAAC
SmaI 8 CCCGGG CCC^GGG
87 CCCGGG
StuI 434 AGGCCT AGG^CCT
509 AGGCCT
TaqI 354 TCGA T^CGA
906 TCGA
TthHB8I 354 TCGA T^CGA
906 TCGA
XmaCI 8 CCCGGG C^CCGGG
87 CCCGGG
XmaI 8 CCCGGG C^CCGGG
87 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI AvaII BanII BbvI
BcnI BfaI BmyI BsaI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeI MaeII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TfiI Tru9I TseI Tsp509I
TspRI XhoII