Restriction map for
pF1KB6379
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
Acc65I AccI AccII AclI AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbsI BbvCI BbvI BcgI
BciVI BclI BglI BlnI BpuAI
BsaAI BsaBI BsaI BsaJI BsgI
BsiEI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraIII DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoT14I EcoT22I FauI FbaI FseI
FspI HincII HindII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MluNI MscI MslI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI TseI Tth111I XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 16 GACGTC GACGT^C
AccIII 26 TCCGGA T^CCGGA
AcyI 16 GACGTC G[GA]^CG[CT]C
AfaI 407 GTAC GT^AC
AlwNI 175 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 337 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 113 GTGCTC G[AT]GC[AT]^C
BanI 544 GGCACC G^G[CT][GA]CC
BcnI 588 CCGGG CC^[GC]GG
BglII 354 AGATCT A^GATCT
BlpI 264 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 264 GCTCAGC GC^T[ACGT]AGC
BsaHI 16 GACGTC G[GA]^CG[CT]C
BsaWI 26 TCCGGA [AT]^CCGG[AT]
BseAI 26 TCCGGA T^CCGGA
BsiHKAI 113 GTGCTC G[AT]GC[AT]^C
BsmAI 483 GTCTC GTCTC(1/5)
BspEI 26 TCCGGA T^CCGGA
BsrI 404 CCAGT ACTGG(1/-1)
BstXI 548 CCATCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 624 GCTAGC GC[ACGT]^[ACGT]GC
CelII 264 GCTCAGC GC^T[ACGT]AGC
DrdI 16 GACGTCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 278 CCTCATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 85 GATATC GAT^ATC
HaeII 72 GGCGCT [GA]GCGC^[CT]
HgaI 162 GCGTC GACGC(5/10)
Hin1I 16 GACGTC G[GA]^CG[CT]C
MroI 26 TCCGGA T^CCGGA
MspA1I 553 CAGCTG C[AC]G^C[GT]G
MwoI 453 GCAGGTCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 588 CCGGG CC^[GC]GG
NheI 624 GCTAGC G^CTAGC
PflMI 234 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 553 CAGCTG CAG^CTG
RsaI 407 GTAC GT^AC
StuI 275 AGGCCT AGG^CCT
TaqI 317 TCGA T^CGA
TspRI 414 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 317 TCGA T^CGA
Van91I 234 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 324 TCTAGA T^CTAGA
XcmI 380 CCAAAGAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 337 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 4 GCGG CCGC(-3/-1)
666 GCGG
AflII 108 CTTAAG C^TTAAG
360 CTTAAG
BfrI 108 CTTAAG C^TTAAG
360 CTTAAG
BmyI 113 GTGCTC G[AGT]GC[ACT]^C
543 GGGCAC
BseRI 350 GAGGAG GAGGAG(10/8)
469 GAGGAG
BsiYI 234 CCAGGAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
278 CCTCATGGAGG
BslI 234 CCAGGAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
278 CCTCATGGAGG
Bsp1286I 113 GTGCTC G[AGT]GC[ACT]^C
543 GGGCAC
BspMI 301 ACCTGC ACCTGC(4/8)
453 GCAGGT
BstYI 29 GGATCT [GA]^GATC[CT]
354 AGATCT
CfoI 73 GCGC GCG^C
622 GCGC
DdeI 265 CTCAG C^T[ACGT]AG
416 CTGAG
DraII 102 AGGACCC [GA]G^G[ACGT]CC[CT]
176 AGGACCT
EcoO109I 102 AGGACCC [GA]G^G[ACGT]CC[CT]
176 AGGACCT
Fnu4HI 4 GCGGC GC^[ACGT]GC
666 GCGGC
HhaI 73 GCGC GCG^C
622 GCGC
HinP1I 73 GCGC G^CGC
622 GCGC
HindIII 565 AAGCTT A^AGCTT
604 AAGCTT
HinfI 211 GAATC G^A[ACGT]TC
246 GAATC
HphI 66 GGTGA GGTGA(8/7)
299 TCACC
ItaI 4 GCGGC GC^[ACGT]GC
666 GCGGC
MaeII 17 ACGT A^CGT
23 ACGT
MflI 29 GGATCT [GA]^GATC[CT]
354 AGATCT
PpuMI 102 AGGACCC [GA]G^G[AT]CC[CT]
176 AGGACCT
SfaNI 261 GATGC GCATC(5/9)
514 GATGC
SmlI 108 CTTAAG C^T[CT][GA]AG
360 CTTAAG
TfiI 211 GAATC G^A[AT]TC
246 GAATC
Tsp45I 164 GTCAC ^GT[GC]AC
490 GTCAC
XhoII 29 GGATCT [GA]^GATC[CT]
354 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AcsI AluI AlwI ApoI AvaII
BfaI BpmI BssKI BstF5I BstNI
Cfr13I DpnII EcoRII FokI HaeIII
HapII HpaII MaeI MaeIII MboI
MboII MnlI MseI MspI MvaI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI Tru9I Tsp509I