Restriction map for
pF1KB8539
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# NO CUT ENZYMES ( 78 enzymes) #
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AclI AflII AflIII AgeI AhdI
AscI AseI AsnI AspEI AvrII
BamHI BbrPI BciVI BclI BfaI
BfrI BglII BlnI BsaBI BsiWI
BspDI BspHI BspLU11I Bst1107I BstBI
BstYI BstZ17I ClaI CpoI DraI
DraIII DrdI Eam1105I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI HpaI
KspI MaeI MamI MfeI MflI
MluI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SapI ScaI SfuI SgrAI
SnaBI SpeI SphI SspI SwaI
XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 1953 GTCGAC GT^[AC][GT]AC
AcsI 2301 GAATTT [GA]^AATT[CT]
Alw44I 1059 GTGCAC G^TGCAC
AocI 2122 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2564 AGCGCT AGC^GCT
ApaI 2086 GGGCCC GGGCC^C
ApaLI 1059 GTGCAC G^TGCAC
ApoI 2301 GAATTT [GA]^AATT[CT]
Asp700I 2604 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 2476 GGCGCC GGCGC^C
BbsI 2053 GTCTTC GAAGAC(2/6)
BcgI 432 CGAGGCCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 2577 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2577 GCTGAGC GC^T[ACGT]AGC
BpuAI 2053 GTCTTC GAAGAC(2/6)
BsmI 903 GCATTC GAATGC(1/-1)
Bsp1407I 524 TGTACA T^GTACA
BsrGI 524 TGTACA T^GTACA
BssHII 2390 GCGCGC G^CGCGC
BssSI 1477 CACGAG CACGAG(-5/-1)
BstAPI 2400 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 280 GGTTACC G^GT[ACGT]ACC
BstPI 280 GGTTACC G^GT[ACGT]ACC
Bsu36I 2122 CCTGAGG CC^T[ACGT]AGG
CelII 2577 GCTGAGC GC^T[ACGT]AGC
Eco47III 2564 AGCGCT AGC^GCT
Eco81I 2122 CCTGAGG CC^T[ACGT]AGG
EcoO65I 280 GGTTACC G^GT[ACGT]ACC
HincII 1953 GTCGAC GT[CT]^[GA]AC
HindII 1953 GTCGAC GT[CT]^[GA]AC
HindIII 2653 AAGCTT A^AGCTT
KasI 2476 GGCGCC G^GCGCC
MseI 89 TTAA T^TAA
NarI 2476 GGCGCC GG^CGCC
PshAI 669 GACGTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 1953 GTCGAC G^TCGAC
SbfI 2742 CCTGCAGG CCTGCA^GG
SexAI 542 ACCTGGT A^CC[AT]GGT
SfiI 1284 GGCCATCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2476 GGCGCC GGC^GCC
SmaI 2325 CCCGGG CCC^GGG
SmlI 1129 CTCAAG C^T[CT][GA]AG
Sse8387I 2742 CCTGCAGG CCTGCA^GG
SspBI 524 TGTACA T^GTACA
Tru9I 89 TTAA T^TAA
XcmI 2618 CCAACCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2325 CCCGGG C^CCGGG
XmaI 2325 CCCGGG C^CCGGG
XmnI 2604 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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Acc65I 1232 GGTACC G^GTACC
1385 GGTACC
AccIII 59 TCCGGA T^CCGGA
2145 TCCGGA
Asp718I 1232 GGTACC G^GTACC
1385 GGTACC
AspI 484 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1111 GACACCGTC
AviII 1706 TGCGCA TGC^GCA
2807 TGCGCA
BbvCI 1755 CCTCAGC CCTCAGC(-5/-2)
2109 CCTCAGC
BsaAI 1324 TACGTG [CT]AC^GT[GA]
1366 TACGTG
BseAI 59 TCCGGA T^CCGGA
2145 TCCGGA
BspEI 59 TCCGGA T^CCGGA
2145 TCCGGA
FspI 1706 TGCGCA TGC^GCA
2807 TGCGCA
KpnI 1232 GGTACC GGTAC^C
1385 GGTACC
MroI 59 TCCGGA T^CCGGA
2145 TCCGGA
NcoI 551 CCATGG C^CATGG
2627 CCATGG
NspI 1055 GCATGT [GA]CATG^[CT]
2258 GCATGT
PflFI 484 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1111 GACACCGTC
PflMI 594 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2622 CCACCCCATGG
TfiI 95 GAATC G^A[AT]TC
240 GAATC
Tsp509I 87 AATT ^AATT
2302 AATT
Tth111I 484 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1111 GACACCGTC
Van91I 594 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2622 CCACCCCATGG
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# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AluI AlwI AlwNI AspHI AvaI
AvaII BalI BanI BanII BbvI
BcnI BglI BmyI BpmI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI TseI Tsp45I TspRI
TthHB8I