Restriction map for
pF1KB8492
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# NO CUT ENZYMES ( 79 enzymes) #
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AccIII AclI AflII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I AviII AvrII BamHI BciVI
BfaI BfrI BlnI BlpI Bpu1102I
BsaWI BseAI BsiWI BsmI BspEI
Bst1107I BstBI BstEII BstPI BstZ17I
CelII CpoI DraI EagI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HindIII HpaI
MaeI MfeI MluI MroI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RsrII SapI
SbfI ScaI SexAI SfuI SnaBI
SpeI SphI Sse8387I SspI SwaI
TfiI XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 628 GGTACC G^GTACC
AcsI 915 GAATTT [GA]^AATT[CT]
AocI 2397 CCTGAGG CC^T[ACGT]AGG
ApoI 915 GAATTT [GA]^AATT[CT]
Asp718I 628 GGTACC G^GTACC
AspI 1720 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1642 GGCGCC GGCGC^C
BbvCI 1930 GCTGAGG CCTCAGC(-5/-2)
BcgI 1134 CGACGAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1058 TGATCA T^GATCA
BsaBI 1059 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1724 CGGTCG CG[GA][CT]^CG
Bsp1407I 1805 TGTACA T^GTACA
BspLU11I 62 ACATGT A^CATGT
BsrDI 438 CATTGC GCAATG(2/0)
BsrGI 1805 TGTACA T^GTACA
BssHII 1894 GCGCGC G^CGCGC
BssSI 1414 CTCGTG CACGAG(-5/-1)
Bsu36I 2397 CCTGAGG CC^T[ACGT]AGG
DraIII 2072 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1720 GACCCGGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2397 CCTGAGG CC^T[ACGT]AGG
FbaI 1058 TGATCA T^GATCA
KasI 1642 GGCGCC G^GCGCC
KpnI 628 GGTACC GGTAC^C
KspI 2145 CCGCGG CCGC^GG
MamI 1059 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 1154 TTAA T^TAA
NarI 1642 GGCGCC GG^CGCC
PflFI 1720 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1216 GACAAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 499 CTGCAG CTGCA^G
SacII 2145 CCGCGG CCGC^GG
SalI 325 GTCGAC G^TCGAC
SfcI 499 CTGCAG C^T[GA][CT]AG
SfiI 1521 GGCCGTGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1642 GGCGCC GGC^GCC
SgrAI 1644 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 1882 CTCAAG C^T[CT][GA]AG
SspBI 1805 TGTACA T^GTACA
StuI 2354 AGGCCT AGG^CCT
Tru9I 1154 TTAA T^TAA
Tth111I 1720 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 33 enzymes) #
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AatII 2107 GACGTC GACGT^C
2116 GACGTC
AccI 16 GTAGAC GT^[AC][GT]AC
325 GTCGAC
AflIII 62 ACATGT A^C[GA][CT]GT
2306 ACGTGT
AhdI 388 GACTGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2395 GACCTGAGGTC
AlwNI 1429 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
2452 CAGATCCTG
AspEI 388 GACTGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2395 GACCTGAGGTC
BalI 698 TGGCCA TGG^CCA
1580 TGGCCA
BbrPI 79 CACGTG CAC^GTG
2305 CACGTG
BbsI 1440 GAAGAC GAAGAC(2/6)
1246 GTCTTC
BglII 1664 AGATCT A^GATCT
1943 AGATCT
BpuAI 1440 GAAGAC GAAGAC(2/6)
1246 GTCTTC
BspDI 613 ATCGAT AT^CGAT
1849 ATCGAT
BspHI 608 TCATGA T^CATGA
911 TCATGA
BsrBI 588 GAGCGG CCGCTC(-3/-3)
742 GAGCGG
ClaI 613 ATCGAT AT^CGAT
1849 ATCGAT
DdeI 1931 CTGAG C^T[ACGT]AG
2398 CTGAG
Eam1105I 388 GACTGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2395 GACCTGAGGTC
EarI 229 CTCTTC CTCTTC(1/4)
1753 GAAGAG
HaeII 1642 GGCGCC [GA]GCGC^[CT]
2177 GGCGCT
Ksp632I 229 CTCTTC CTCTTC(1/4)
1753 GAAGAG
MluNI 698 TGGCCA TGG^CCA
1580 TGGCCA
MscI 698 TGGCCA TGG^CCA
1580 TGGCCA
PflMI 1428 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2496 CCAGGTGGTGG
PmaCI 79 CACGTG CAC^GTG
2305 CACGTG
PmlI 79 CACGTG CAC^GTG
2305 CACGTG
PpuMI 1319 AGGACCT [GA]G^G[AT]CC[CT]
1676 AGGACCT
PvuII 502 CAGCTG CAG^CTG
871 CAGCTG
RcaI 608 TCATGA T^CATGA
911 TCATGA
SmaI 146 CCCGGG CCC^GGG
316 CCCGGG
Tsp509I 916 AATT ^AATT
2570 AATT
Van91I 1428 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2496 CCAGGTGGTGG
XmaCI 146 CCCGGG C^CCGGG
316 CCCGGG
XmaI 146 CCCGGG C^CCGGG
316 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaI AvaII
BanI BanII BbvI BcnI BglI
BmyI BpmI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DpnII DraII DsaI EaeI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PleI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TseI Tsp45I TspRI
TthHB8I XcmI XhoII