Restriction map for
pF1KB8481
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
AocI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BalI BbrPI BciVI BclI BglI
BglII BlnI BsaAI BsaBI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrDI BsrGI BstBI BstXI
Bsu36I ClaI CpoI DraI Eam1105I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HpaI
KpnI MamI MfeI MluNI MscI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccIII 262 TCCGGA T^CCGGA
AcsI 1351 AAATTT [GA]^AATT[CT]
AflII 1336 CTTAAG C^TTAAG
Aor51HI 560 AGCGCT AGC^GCT
ApoI 1351 AAATTT [GA]^AATT[CT]
AvaI 63 CTCGAG C^[CT]CG[GA]G
AviII 617 TGCGCA TGC^GCA
BanII 2508 GAGCCC G[GA]GC[CT]^C
BbeI 962 GGCGCC GGCGC^C
BbvCI 1065 CCTCAGC CCTCAGC(-5/-2)
BcnI 268 CCCGG CC^[GC]GG
BfrI 1336 CTTAAG C^TTAAG
BsaWI 262 TCCGGA [AT]^CCGG[AT]
BseAI 262 TCCGGA T^CCGGA
BsiWI 446 CGTACG C^GTACG
BsoBI 63 CTCGAG C^[CT]CG[GA]G
BspEI 262 TCCGGA T^CCGGA
Bst1107I 1269 GTATAC GTA^TAC
BstAPI 672 GCAGTCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1269 GTATAC GTA^TAC
DraIII 1116 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2395 GACATAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 123 CCGCGG C^C[GA][CT]GG
EagI 905 CGGCCG C^GGCCG
EclXI 905 CGGCCG C^GGCCG
Eco47III 560 AGCGCT AGC^GCT
Eco52I 905 CGGCCG C^GGCCG
FspI 617 TGCGCA TGC^GCA
HindIII 1333 AAGCTT A^AGCTT
KasI 962 GGCGCC G^GCGCC
KspI 123 CCGCGG CCGC^GG
MluI 443 ACGCGT A^CGCGT
MroI 262 TCCGGA T^CCGGA
MslI 759 CATCACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 337 GCCGGC GCC^GGC
NarI 962 GGCGCC GG^CGCC
NciI 268 CCCGG CC^[GC]GG
NgoMIV 337 GCCGGC G^CCGGC
NspI 164 GCATGC [GA]CATG^[CT]
PaeR7I 63 CTCGAG C^TCGAG
PflMI 1089 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1154 AGGACCT [GA]G^G[AT]CC[CT]
SacII 123 CCGCGG CCGC^GG
SapI 2650 GAAGAGC GCTCTTC(1/4)
SfoI 962 GGCGCC GGC^GCC
SpeI 2679 ACTAGT A^CTAGT
SphI 164 GCATGC GCATG^C
StuI 419 AGGCCT AGG^CCT
TfiI 1305 GATTC G^A[AT]TC
Van91I 1089 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 63 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 1269 GTATAC GT^[AC][GT]AC
2685 GTCTAC
AcyI 636 GGCGTC G[GA]^CG[CT]C
962 GGCGCC
AflIII 8 ACACGT A^C[GA][CT]GT
443 ACGCGT
Alw44I 355 GTGCAC G^TGCAC
2263 GTGCAC
ApaLI 355 GTGCAC G^TGCAC
2263 GTGCAC
AspHI 355 GTGCAC G[AT]GC[AT]^C
2263 GTGCAC
BamHI 1654 GGATCC G^GATCC
2380 GGATCC
BlpI 201 GCTCAGC GC^T[ACGT]AGC
1032 GCTCAGC
Bpu1102I 201 GCTCAGC GC^T[ACGT]AGC
1032 GCTCAGC
BsaHI 636 GGCGTC G[GA]^CG[CT]C
962 GGCGCC
BsiEI 327 CGACCG CG[GA][CT]^CG
905 CGGCCG
BsiHKAI 355 GTGCAC G[AT]GC[AT]^C
2263 GTGCAC
BssHII 471 GCGCGC G^CGCGC
611 GCGCGC
BssSI 496 CACGAG CACGAG(-5/-1)
712 CACGAG
CelII 201 GCTCAGC GC^T[ACGT]AGC
1032 GCTCAGC
DraII 1154 AGGACCT [GA]G^G[ACGT]CC[CT]
1412 AGGCCCT
EcoO109I 1154 AGGACCT [GA]G^G[ACGT]CC[CT]
1412 AGGCCCT
Hin1I 636 GGCGTC G[GA]^CG[CT]C
962 GGCGCC
MaeII 10 ACGT A^CGT
1166 ACGT
Tsp509I 1352 AATT ^AATT
1436 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaII BanI BbsI BbvI
BcgI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrBI
BsrFI BsrI BssKI BstEII BstF5I
BstNI BstPI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
EaeI EarI EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI Tru9I TseI Tsp45I TspRI
TthHB8I XcmI XhoII