Restriction map for
pF1KB5709
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# NO CUT ENZYMES ( 102 enzymes) #
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AatII Acc65I AccI AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AocI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AvrII BbeI
BbrPI BcgI BciVI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsiWI BsmBI BsmI
BspHI BsrDI BsrFI BssHII BssSI
Bst1107I BstBI BstZ17I Bsu36I CelII
Cfr10I CpoI DraI DraIII Eam1105I
Eco81I EcoRI EcoRV EcoT22I FseI
Hin1I HindIII HpaI KasI KpnI
MaeII MamI MfeI MluI MunI
NaeI NarI NdeI NgoMIV NheI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI StuI SwaI XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 1425 CGCG CG^CG
AccIII 515 TCCGGA T^CCGGA
AflIII 266 ACATGT A^C[GA][CT]GT
AlwNI 2183 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1826 AGCGCT AGC^GCT
AspI 1635 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1259 CTCGAG C^[CT]CG[GA]G
AviII 1445 TGCGCA TGC^GCA
BamHI 743 GGATCC G^GATCC
BanI 453 GGTGCC G^G[CT][GA]CC
BclI 1070 TGATCA T^GATCA
BcnI 1523 CCCGG CC^[GC]GG
BglII 1004 AGATCT A^GATCT
BseAI 515 TCCGGA T^CCGGA
BsiEI 2268 CGGCCG CG[GA][CT]^CG
BsoBI 1259 CTCGAG C^[CT]CG[GA]G
Bsp1407I 754 TGTACA T^GTACA
BspDI 2227 ATCGAT AT^CGAT
BspEI 515 TCCGGA T^CCGGA
BspLU11I 266 ACATGT A^CATGT
BsrGI 754 TGTACA T^GTACA
BstAPI 1566 GCAGATGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2122 GGTAACC G^GT[ACGT]ACC
BstPI 2122 GGTAACC G^GT[ACGT]ACC
BstUI 1425 CGCG CG^CG
ClaI 2227 ATCGAT AT^CGAT
EagI 2268 CGGCCG C^GGCCG
EclXI 2268 CGGCCG C^GGCCG
Eco47III 1826 AGCGCT AGC^GCT
Eco52I 2268 CGGCCG C^GGCCG
EcoO65I 2122 GGTAACC G^GT[ACGT]ACC
FbaI 1070 TGATCA T^GATCA
FspI 1445 TGCGCA TGC^GCA
HaeII 1826 AGCGCT [GA]GCGC^[CT]
HgaI 988 GACGC GACGC(5/10)
KspI 1424 CCGCGG CCGC^GG
MroI 515 TCCGGA T^CCGGA
MvnI 1425 CGCG CG^CG
NciI 1523 CCCGG CC^[GC]GG
NotI 2267 GCGGCCGC GC^GGCCGC
PaeR7I 1259 CTCGAG C^TCGAG
PflFI 1635 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 1424 CCGCGG CCGC^GG
SphI 1844 GCATGC GCATG^C
SspBI 754 TGTACA T^GTACA
Tth111I 1635 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1268 CCAACGGGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1259 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 755 GTAC GT^AC
1980 GTAC
Asp700I 501 GAATAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2028 GAATCCATTC
BalI 1818 TGGCCA TGG^CCA
2327 TGGCCA
BfaI 335 CTAG C^TAG
1723 CTAG
BsaWI 515 TCCGGA [AT]^CCGG[AT]
740 ACCGGA
BsmAI 919 GAGAC GTCTC(1/5)
1200 GAGAC
BspMI 1463 ACCTGC ACCTGC(4/8)
1547 ACCTGC
BsrBI 1372 CCGCTC CCGCTC(-3/-3)
1262 GAGCGG
BstXI 279 CCAGGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1901 CCAGCCCCATGG
CfoI 1446 GCGC GCG^C
1827 GCGC
DrdI 121 GACCGTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
469 GACCAGGATGTC
EcoNI 1933 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2274 CCTGGATGAGG
HhaI 1446 GCGC GCG^C
1827 GCGC
HinP1I 1446 GCGC G^CGC
1827 GCGC
HincII 463 GTCAAC GT[CT]^[GA]AC
865 GTTGAC
HindII 463 GTCAAC GT[CT]^[GA]AC
865 GTTGAC
HphI 605 TCACC GGTGA(8/7)
655 TCACC
MaeI 335 CTAG C^TAG
1723 CTAG
MluNI 1818 TGGCCA TGG^CCA
2327 TGGCCA
MscI 1818 TGGCCA TGG^CCA
2327 TGGCCA
NcoI 669 CCATGG C^CATGG
1907 CCATGG
RsaI 755 GTAC GT^AC
1980 GTAC
SacI 574 GAGCTC GAGCT^C
2209 GAGCTC
SapI 2206 GAAGAGC GCTCTTC(1/4)
1727 GCTCTTC
XmnI 501 GAATAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2028 GAATCCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AluI AlwI ApaI AspHI
AvaII BanII BbsI BbvCI BbvI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII