Restriction map for
pF1KB9986
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# NO CUT ENZYMES ( 129 enzymes) #
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Acc65I AccIII AclI AgeI AhdI
Alw44I AlwI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BcgI BclI BcnI BlnI BpmI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsmFI BsoBI BspDI
BspEI BsrBI BsrFI BssHII Bst1107I
BstEII BstPI BstXI BstZ17I Cfr10I
ClaI CpoI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV FauI
FbaI FseI FspI HaeII HapII
HgaI HpaI HpaII KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MspI MunI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SacII SapI
SbfI SexAI SfcI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AatII 1100 GACGTC GACGT^C
AccII 1787 CGCG CG^CG
AcyI 1100 GACGTC G[GA]^CG[CT]C
AflII 1020 CTTAAG C^TTAAG
AflIII 1321 ACATGT A^C[GA][CT]GT
AocI 109 CCTGAGG CC^T[ACGT]AGG
AspHI 847 GAGCAC G[AT]GC[AT]^C
AvaII 585 GGACC G^G[AT]CC
BbvCI 1377 GCTGAGG CCTCAGC(-5/-2)
BciVI 1284 GTATCC GTATCC(6/5)
BfrI 1020 CTTAAG C^TTAAG
BglI 1554 GCCAAAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 62 AGATCT A^GATCT
BlpI 597 GCTAAGC GC^T[ACGT]AGC
BmyI 847 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 597 GCTAAGC GC^T[ACGT]AGC
BsaAI 1123 TACGTG [CT]AC^GT[GA]
BsaHI 1100 GACGTC G[GA]^CG[CT]C
BsaJI 1458 CCTTGG C^C[ACGT][ACGT]GG
BsiHKAI 847 GAGCAC G[AT]GC[AT]^C
BsmI 1865 GCATTC GAATGC(1/-1)
Bsp1286I 847 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 1318 TGTACA T^GTACA
BspLU11I 1321 ACATGT A^CATGT
BspMI 1211 ACCTGC ACCTGC(4/8)
BsrDI 404 CATTGC GCAATG(2/0)
BsrGI 1318 TGTACA T^GTACA
BssSI 1936 CTCGTG CACGAG(-5/-1)
BstAPI 1993 GCAAATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 130 TTCGAA TT^CGAA
BstUI 1787 CGCG CG^CG
BstYI 62 AGATCT [GA]^GATC[CT]
Bsu36I 109 CCTGAGG CC^T[ACGT]AGG
CelII 597 GCTAAGC GC^T[ACGT]AGC
CfoI 1156 GCGC GCG^C
DraII 368 AGGCCCT [GA]G^G[ACGT]CC[CT]
Eco81I 109 CCTGAGG CC^T[ACGT]AGG
EcoO109I 368 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 258 GAATTC G^AATTC
EcoT14I 1458 CCTTGG C^C[AT][AT]GG
EcoT22I 1358 ATGCAT ATGCA^T
HhaI 1156 GCGC GCG^C
Hin1I 1100 GACGTC G[GA]^CG[CT]C
HinP1I 1156 GCGC G^CGC
MflI 62 AGATCT [GA]^GATC[CT]
MvnI 1787 CGCG CG^CG
NlaIV 585 GGACCC GG[ACGT]^[ACGT]CC
NsiI 1358 ATGCAT ATGCA^T
NspV 130 TTCGAA TT^CGAA
PflMI 1728 CCATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SalI 1295 GTCGAC G^TCGAC
SfuI 130 TTCGAA TT^CGAA
SspBI 1318 TGTACA T^GTACA
StyI 1458 CCTTGG C^C[AT][AT]GG
SwaI 1772 ATTTAAAT ATTT^AAAT
Van91I 1728 CCATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 62 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 1103 GTCTAC GT^[AC][GT]AC
1295 GTCGAC
AciI 1786 CCGC CCGC(-3/-1)
275 GCGG
BsgI 1805 CTGCAC GTGCAG(16/14)
1906 CTGCAC
BsiYI 852 CCTGACTACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1728 CCATGAGGTGG
BslI 852 CCTGACTACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1728 CCATGAGGTGG
BspHI 339 TCATGA T^CATGA
948 TCATGA
BssKI 1239 CCTGG ^CC[ACGT]GG
1491 CCTGG
BstNI 1239 CCTGG CC^[AT]GG
1491 CCTGG
DraI 898 TTTAAA TTT^AAA
1773 TTTAAA
EarI 1269 CTCTTC CTCTTC(1/4)
1274 CTCTTC
EcoRII 1239 CCTGG ^CC[AT]GG
1491 CCTGG
HaeIII 369 GGCC GG^CC
1494 GGCC
HincII 713 GTCAAC GT[CT]^[GA]AC
1295 GTCGAC
HindII 713 GTCAAC GT[CT]^[GA]AC
1295 GTCGAC
HphI 515 TCACC GGTGA(8/7)
1265 TCACC
Ksp632I 1269 CTCTTC CTCTTC(1/4)
1274 CTCTTC
MslI 718 CATCTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1771 CATTTAAATG
MspA1I 1372 CAGCTG C[AC]G^C[GT]G
1973 CAGCTG
MvaI 1239 CCTGG CC^[AT]GG
1491 CCTGG
NspI 1215 GCATGT [GA]CATG^[CT]
1321 ACATGT
PvuII 1372 CAGCTG CAG^CTG
1973 CAGCTG
RcaI 339 TCATGA T^CATGA
948 TCATGA
ScaI 684 AGTACT AGT^ACT
1650 AGTACT
ScrFI 1239 CCTGG CC^[ACGT]GG
1491 CCTGG
TspRI 1746 CAGTG CA[GC]TG[ACGT][ACGT]^
1844 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AcsI AfaI AluI AlwNI ApoI
Asp700I BbsI BbvI BfaI BpuAI
BseRI BsmAI BsrI BstF5I Cac8I
Cfr13I DdeI DpnII Fnu4HI FokI
HindIII HinfI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MwoI NdeI NdeII NlaIII RsaI
Sau3AI Sau96I SfaNI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TthHB8I XmnI