Restriction map for
pF1KB8503
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AccII AclI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI AscI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BanI
BbeI BbrPI BcgI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsiEI BsiWI
BsmBI BsoBI BspDI BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstEII
BstPI BstUI BstXI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO109I EcoO65I FauI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I KasI KpnI KspI
MamI MluI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccIII 2547 TCCGGA T^CCGGA
AflIII 2371 ACATGT A^C[GA][CT]GT
AlwNI 1559 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 580 CCTCAGG CC^T[ACGT]AGG
ApaI 2494 GGGCCC GGGCC^C
AseI 799 ATTAAT AT^TAAT
AsnI 799 ATTAAT AT^TAAT
AvaII 1693 GGTCC G^G[AT]CC
BalI 1902 TGGCCA TGG^CCA
BamHI 2544 GGATCC G^GATCC
BbvCI 682 GCTGAGG CCTCAGC(-5/-2)
BciVI 725 GGATAC GTATCC(6/5)
BclI 2382 TGATCA T^GATCA
BsaI 1766 GAGACC GGTCTC(1/5)
BsaWI 2547 TCCGGA [AT]^CCGG[AT]
BseAI 2547 TCCGGA T^CCGGA
BseRI 1471 GAGGAG GAGGAG(10/8)
BsgI 749 GTGCAG GTGCAG(16/14)
BsmI 1716 GAATGC GAATGC(1/-1)
Bsp1407I 2374 TGTACA T^GTACA
BspEI 2547 TCCGGA T^CCGGA
BspHI 204 TCATGA T^CATGA
BspLU11I 2371 ACATGT A^CATGT
BsrGI 2374 TGTACA T^GTACA
BstAPI 694 GCAGATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 2544 GGATCC [GA]^GATC[CT]
Bsu36I 580 CCTCAGG CC^T[ACGT]AGG
DsaI 2498 CCGTGG C^C[GA][CT]GG
EaeI 1902 TGGCCA [CT]^GGCC[GA]
Eco81I 580 CCTCAGG CC^T[ACGT]AGG
EcoNI 295 CCTGTGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 2382 TGATCA T^GATCA
HapII 2548 CCGG C^CGG
HpaII 2548 CCGG C^CGG
MaeII 2770 ACGT A^CGT
MfeI 1534 CAATTG C^AATTG
MflI 2544 GGATCC [GA]^GATC[CT]
MluNI 1902 TGGCCA TGG^CCA
MroI 2547 TCCGGA T^CCGGA
MscI 1902 TGGCCA TGG^CCA
MspI 2548 CCGG C^CGG
MunI 1534 CAATTG C^AATTG
PshAI 2624 GACTCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 799 ATTAAT AT^TAAT
PstI 2315 CTGCAG CTGCA^G
RcaI 204 TCATGA T^CATGA
SapI 2680 GAAGAGC GCTCTTC(1/4)
SexAI 1419 ACCAGGT A^CC[AT]GGT
SphI 2742 GCATGC GCATG^C
SspBI 2374 TGTACA T^GTACA
SwaI 398 ATTTAAAT ATTT^AAAT
XhoII 2544 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 1012 GTAGAC GT^[AC][GT]AC
2631 GTCTAC
AciI 1950 CCGC CCGC(-3/-1)
2156 GCGG
BanII 2281 GAGCCC G[GA]GC[CT]^C
2494 GGGCCC
BbsI 2643 GAAGAC GAAGAC(2/6)
1389 GTCTTC
BpuAI 2643 GAAGAC GAAGAC(2/6)
1389 GTCTTC
BstBI 17 TTCGAA TT^CGAA
1966 TTCGAA
DraI 399 TTTAAA TTT^AAA
756 TTTAAA
EcoRI 208 GAATTC G^AATTC
819 GAATTC
EcoRV 1276 GATATC GAT^ATC
2119 GATATC
HincII 2562 GTTAAC GT[CT]^[GA]AC
2772 GTTAAC
HindII 2562 GTTAAC GT[CT]^[GA]AC
2772 GTTAAC
HpaI 2562 GTTAAC GTT^AAC
2772 GTTAAC
MspA1I 1019 CAGCTG C[AC]G^C[GT]G
1688 CAGCTG
NspV 17 TTCGAA TT^CGAA
1966 TTCGAA
PvuII 1019 CAGCTG CAG^CTG
1688 CAGCTG
SfuI 17 TTCGAA TT^CGAA
1966 TTCGAA
StuI 945 AGGCCT AGG^CCT
2069 AGGCCT
XcmI 371 CCATTGATGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2124 CCATGATGCACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AspHI BbvI BfaI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EarI EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeIII HindIII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NsiI NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I