Restriction map for
pF1KB8485
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# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
AvrII BamHI BciVI BfrI BlnI
BsaBI BsiWI BsmBI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstZ17I Bsu36I
ClaI DraI Eco47III Eco81I EcoRI
EcoRV EcoT22I FseI HindIII HpaI
KpnI KspI MamI MfeI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshBI Psp1406I
PvuI RcaI SacII SalI ScaI
SexAI SfuI SmlI SnaBI SpeI
SspBI SspI SwaI Tsp509I Tth111I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 727 GTAGAC GT^[AC][GT]AC
Alw44I 1173 GTGCAC G^TGCAC
ApaI 73 GGGCCC GGGCC^C
ApaLI 1173 GTGCAC G^TGCAC
BbrPI 1051 CACGTG CAC^GTG
BbvCI 183 GCTGAGG CCTCAGC(-5/-2)
BglII 1691 AGATCT A^GATCT
BsaI 1453 GAGACC GGTCTC(1/5)
BsiEI 1166 CGGCCG CG[GA][CT]^CG
BsmI 1348 GAATGC GAATGC(1/-1)
BspMI 2412 GCAGGT ACCTGC(4/8)
BssSI 2762 CTCGTG CACGAG(-5/-1)
BstEII 2727 GGTCACC G^GT[ACGT]ACC
BstPI 2727 GGTCACC G^GT[ACGT]ACC
CpoI 212 CGGTCCG CG^G[AT]CCG
DrdI 1998 GACCATGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1166 CGGCCG C^GGCCG
EclXI 1166 CGGCCG C^GGCCG
Eco52I 1166 CGGCCG C^GGCCG
EcoO65I 2727 GGTCACC G^GT[ACGT]ACC
HincII 2757 GTTGAC GT[CT]^[GA]AC
HindII 2757 GTTGAC GT[CT]^[GA]AC
MluI 743 ACGCGT A^CGCGT
PmaCI 1051 CACGTG CAC^GTG
PmlI 1051 CACGTG CAC^GTG
PshAI 2760 GACTCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 598 CAGCTG CAG^CTG
RsrII 212 CGGTCCG CG^G[AT]CCG
SacI 2083 GAGCTC GAGCT^C
SapI 2572 GAAGAGC GCTCTTC(1/4)
SfiI 291 GGCCACAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1435 CACCGGCG C[GA]^CCGG[CT]G
SmaI 70 CCCGGG CCC^GGG
SphI 1559 GCATGC GCATG^C
TfiI 897 GAATC G^A[AT]TC
XmaCI 70 CCCGGG C^CCGGG
XmaI 70 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 215 TCCGGA T^CCGGA
2087 TCCGGA
AhdI 883 GACCATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2206 GACCAGGCGTC
AspEI 883 GACCATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2206 GACCAGGCGTC
AviII 456 TGCGCA TGC^GCA
2751 TGCGCA
BbeI 1439 GGCGCC GGCGC^C
2146 GGCGCC
BbsI 787 GAAGAC GAAGAC(2/6)
15 GTCTTC
BcgI 642 CGACGTGGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2754 GCAGTTGACTCG
BclI 1482 TGATCA T^GATCA
2249 TGATCA
BlpI 1023 GCTGAGC GC^T[ACGT]AGC
2178 GCTCAGC
Bpu1102I 1023 GCTGAGC GC^T[ACGT]AGC
2178 GCTCAGC
BpuAI 787 GAAGAC GAAGAC(2/6)
15 GTCTTC
BseAI 215 TCCGGA T^CCGGA
2087 TCCGGA
BspEI 215 TCCGGA T^CCGGA
2087 TCCGGA
BsrBI 1291 GAGCGG CCGCTC(-3/-3)
1791 GAGCGG
BsrFI 1436 ACCGGC [GA]^CCGG[CT]
2056 ACCGGC
CelII 1023 GCTGAGC GC^T[ACGT]AGC
2178 GCTCAGC
Cfr10I 1436 ACCGGC [GA]^CCGG[CT]
2056 ACCGGC
DraIII 305 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
1747 CACATCGTG
Eam1105I 883 GACCATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2206 GACCAGGCGTC
EarI 2317 CTCTTC CTCTTC(1/4)
2572 GAAGAG
FbaI 1482 TGATCA T^GATCA
2249 TGATCA
FspI 456 TGCGCA TGC^GCA
2751 TGCGCA
KasI 1439 GGCGCC G^GCGCC
2146 GGCGCC
Ksp632I 2317 CTCTTC CTCTTC(1/4)
2572 GAAGAG
MroI 215 TCCGGA T^CCGGA
2087 TCCGGA
MseI 1363 TTAA T^TAA
2299 TTAA
NarI 1439 GGCGCC GG^CGCC
2146 GGCGCC
NcoI 170 CCATGG C^CATGG
1520 CCATGG
SbfI 1104 CCTGCAGG CCTGCA^GG
1317 CCTGCAGG
SfoI 1439 GGCGCC GGC^GCC
2146 GGCGCC
Sse8387I 1104 CCTGCAGG CCTGCA^GG
1317 CCTGCAGG
Tru9I 1363 TTAA T^TAA
2299 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AspHI AvaI
AvaII BalI BanI BanII BbvI
BcnI BfaI BglI BmyI BpmI
BsaAI BsaHI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XhoII