Restriction map for
pF1KB5929
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII AccI AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BcnI BfrI
BlnI BlpI BpmI Bpu1102I BsaAI
BsaHI BseAI BseRI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssKI BssSI Bst1107I BstEII BstNI
BstPI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRII EcoT22I FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
MaeII MluI MluNI MroI MscI
MslI MvaI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SbfI ScrFI
SexAI SfiI SfoI SgrAI SmaI
SmlI SnaBI Sse8387I SspBI SwaI
Tsp45I Tth111I Van91I XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 37 GGTACC G^GTACC
AlwNI 104 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 37 GGTACC G^GTACC
AspHI 365 GTGCTC G[AT]GC[AT]^C
AvaI 24 CTCGGG C^[CT]CG[GA]G
AvaII 1164 GGTCC G^G[AT]CC
BanII 17 GGGCTC G[GA]GC[CT]^C
BbsI 1004 GAAGAC GAAGAC(2/6)
BciVI 1242 GGATAC GTATCC(6/5)
BclI 705 TGATCA T^GATCA
BpuAI 1004 GAAGAC GAAGAC(2/6)
BsaI 304 GGTCTC GGTCTC(1/5)
BsaWI 34 TCCGGT [AT]^CCGG[AT]
BsgI 869 CTGCAC GTGCAG(16/14)
BsiHKAI 365 GTGCTC G[AT]GC[AT]^C
BsmFI 85 GGGAC GGGAC(10/14)
BsmI 112 GCATTC GAATGC(1/-1)
BsoBI 24 CTCGGG C^[CT]CG[GA]G
BspMI 103 GCAGGT ACCTGC(4/8)
BstAPI 103 GCAGGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 605 TTCGAA TT^CGAA
DraII 1163 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 1163 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 487 GAATTC G^AATTC
EcoRV 1054 GATATC GAT^ATC
EcoT14I 935 CCAAGG C^C[AT][AT]GG
FbaI 705 TGATCA T^GATCA
HapII 35 CCGG C^CGG
HpaII 35 CCGG C^CGG
HphI 1255 GGTGA GGTGA(8/7)
KpnI 37 GGTACC GGTAC^C
MfeI 817 CAATTG C^AATTG
MspI 35 CCGG C^CGG
MunI 817 CAATTG C^AATTG
NspI 539 GCATGC [GA]CATG^[CT]
NspV 605 TTCGAA TT^CGAA
PleI 152 GACTC GAGTC(4/5)
PpuMI 1163 AGGTCCT [GA]G^G[AT]CC[CT]
SapI 1219 GAAGAGC GCTCTTC(1/4)
ScaI 220 AGTACT AGT^ACT
SfuI 605 TTCGAA TT^CGAA
SpeI 217 ACTAGT A^CTAGT
SphI 539 GCATGC GCATG^C
SspI 472 AATATT AAT^ATT
StyI 935 CCAAGG C^C[AT][AT]GG
TspRI 482 CAGTG CA[GC]TG[ACGT][ACGT]^
XbaI 1452 TCTAGA T^CTAGA
XcmI 1497 CCACATCAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccII 54 CGCG CG^CG
63 CGCG
AcsI 487 GAATTC [GA]^AATT[CT]
1181 AAATTC
AlwI 913 GATCC GGATC(4/5)
1140 GGATC
ApoI 487 GAATTC [GA]^AATT[CT]
1181 AAATTC
BanI 28 GGCACC G^G[CT][GA]CC
37 GGTACC
BglI 49 GCCACCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1204 GCCATTCAGGC
BglII 1342 AGATCT A^GATCT
1371 AGATCT
BsaBI 1366 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1431 GATTAGCATC
BsmAI 305 GTCTC GTCTC(1/5)
1305 GTCTC
BsrDI 635 CATTGC GCAATG(2/0)
695 CATTGC
BstF5I 1494 CATCC GGATG(2/0)
1316 GGATG
BstUI 54 CGCG CG^CG
63 CGCG
Cac8I 432 GCAGGC GC[ACGT]^[ACGT]GC
539 GCATGC
DsaI 53 CCGCGG C^C[GA][CT]GG
62 CCGCGG
FauI 61 CCCGC CCCGC(4/6)
64 GCGGG
FokI 1494 CATCC GGATG(9/13)
1316 GGATG
KspI 53 CCGCGG CCGC^GG
62 CCGCGG
MaeIII 835 GTAAC ^GT[ACGT]AC
1377 GTTAC
MamI 1366 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1431 GATTAGCATC
MvnI 54 CGCG CG^CG
63 CGCG
PvuII 346 CAGCTG CAG^CTG
1237 CAGCTG
SacII 53 CCGCGG CCGC^GG
62 CCGCGG
StuI 434 AGGCCT AGG^CCT
1416 AGGCCT
TaqI 606 TCGA T^CGA
1472 TCGA
TthHB8I 606 TCGA T^CGA
1472 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AfaI AluI BbvI BfaI
BmyI BsaJI BsiYI BslI Bsp1286I
BsrI BstYI Cfr13I DdeI DpnII
EarI Fnu4HI HaeIII HinfI ItaI
Ksp632I MaeI MboI MboII MflI
MnlI MseI MspA1I MwoI NdeII
NlaIII NlaIV PstI RsaI Sau3AI
Sau96I SfaNI SfcI TfiI Tru9I
TseI Tsp509I XhoII