Restriction map for
pF1KB5901
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflIII AgeI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspI AvaI AvrII BalI BamHI
BbeI BbrPI BbsI BcgI BclI
BcnI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrFI BssHII
BssSI BstBI BstEII BstPI BstXI
BstYI CelII Cfr10I ClaI CpoI
DraIII DrdI EagI EarI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
EcoT14I FbaI FseI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I MamI MfeI MflI
MluI MluNI MroI MscI MunI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI StuI
StyI SwaI Tth111I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 1011 GTATAC GT^[AC][GT]AC
AflII 868 CTTAAG C^TTAAG
AhdI 734 GACAAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 758 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 545 CCTCAGG CC^T[ACGT]AGG
ApaI 1032 GGGCCC GGGCC^C
AspEI 734 GACAAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 753 GTGCTC G[AT]GC[AT]^C
AviII 39 TGCGCA TGC^GCA
BanI 1401 GGTGCC G^G[CT][GA]CC
BbvCI 468 CCTCAGC CCTCAGC(-5/-2)
BfaI 95 CTAG C^TAG
BfrI 868 CTTAAG C^TTAAG
BseRI 28 GAGGAG GAGGAG(10/8)
BsgI 1364 GTGCAG GTGCAG(16/14)
BsiHKAI 753 GTGCTC G[AT]GC[AT]^C
BsmAI 288 GTCTC GTCTC(1/5)
BsmI 1228 GCATTC GAATGC(1/-1)
Bsp1407I 1181 TGTACA T^GTACA
BsrBI 70 GAGCGG CCGCTC(-3/-3)
BsrGI 1181 TGTACA T^GTACA
Bst1107I 1011 GTATAC GTA^TAC
BstAPI 157 GCAAAAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1011 GTATAC GTA^TAC
Bsu36I 545 CCTCAGG CC^T[ACGT]AGG
DraI 117 TTTAAA TTT^AAA
DraII 1032 GGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 1351 CGGCCA [CT]^GGCC[GA]
Eam1105I 734 GACAAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 545 CCTCAGG CC^T[ACGT]AGG
EcoO109I 1032 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 700 GAATTC G^AATTC
EcoT22I 537 ATGCAT ATGCA^T
FauI 22 CCCGC CCCGC(4/6)
FspI 39 TGCGCA TGC^GCA
HaeII 973 GGCGCT [GA]GCGC^[CT]
HapII 1254 CCGG C^CGG
HpaII 1254 CCGG C^CGG
HphI 67 GGTGA GGTGA(8/7)
MaeI 95 CTAG C^TAG
MaeII 1100 ACGT A^CGT
MspI 1254 CCGG C^CGG
NdeI 176 CATATG CA^TATG
NsiI 537 ATGCAT ATGCA^T
PflMI 49 CCATCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScaI 141 AGTACT AGT^ACT
SfaNI 823 GATGC GCATC(5/9)
SfcI 1481 CTACAG C^T[GA][CT]AG
SphI 42 GCATGC GCATG^C
SspBI 1181 TGTACA T^GTACA
Van91I 49 CCATCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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BanII 15 GGGCTC G[GA]GC[CT]^C
1032 GGGCCC
BciVI 873 GGATAC GTATCC(6/5)
1495 GTATCC
BsmFI 1097 GGGAC GGGAC(10/14)
1213 GTCCC
CfoI 40 GCGC GCG^C
974 GCGC
DsaI 23 CCGCGG C^C[GA][CT]GG
1348 CCGCGG
HhaI 40 GCGC GCG^C
974 GCGC
HinP1I 40 GCGC G^CGC
974 GCGC
KspI 23 CCGCGG CCGC^GG
1348 CCGCGG
MspA1I 23 CCGCGG C[AC]G^C[GT]G
1348 CCGCGG
NspI 42 GCATGC [GA]CATG^[CT]
609 GCATGT
PleI 394 GACTC GAGTC(4/5)
286 GAGTC
SacII 23 CCGCGG CCGC^GG
1348 CCGCGG
SmlI 868 CTTAAG C^T[CT][GA]AG
902 CTCAAG
TaqI 573 TCGA T^CGA
1383 TCGA
TthHB8I 573 TCGA T^CGA
1383 TCGA
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AvaII BbvI BmyI
BsaJI BsiYI BslI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstUI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
MaeIII MboI MboII MnlI MseI
MslI MvaI MvnI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI