################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BbrPI BbsI BbvCI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaWI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MluI MroI MseI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI SspBI SspI StuI SwaI Tru9I Tsp509I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccII 157 CGCG CG^CG AcyI 1164 GGCGCC G[GA]^CG[CT]C Alw44I 198 GTGCAC G^TGCAC AlwNI 560 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 198 GTGCAC G^TGCAC AspI 445 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1014 TGGCCA TGG^CCA BamHI 378 GGATCC G^GATCC BbeI 1164 GGCGCC GGCGC^C BcgI 995 GCAGCCTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 992 CTAG C^TAG BglI 29 GCCCGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 169 TACGTG [CT]AC^GT[GA] BsaHI 1164 GGCGCC G[GA]^CG[CT]C BsaI 692 GAGACC GGTCTC(1/5) BseRI 645 CTCCTC GAGGAG(10/8) BsmBI 551 CGTCTC CGTCTC(1/5) BsmI 733 GCATTC GAATGC(1/-1) BspHI 1382 TCATGA T^CATGA BsrBI 1027 CCGCTC CCGCTC(-3/-3) BstEII 162 GGTCACC G^GT[ACGT]ACC BstPI 162 GGTCACC G^GT[ACGT]ACC BstUI 157 CGCG CG^CG EaeI 1014 TGGCCA [CT]^GGCC[GA] EcoNI 760 CCTTCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 162 GGTCACC G^GT[ACGT]ACC EcoRV 175 GATATC GAT^ATC EcoT14I 765 CCAAGG C^C[AT][AT]GG HaeII 1164 GGCGCC [GA]GCGC^[CT] Hin1I 1164 GGCGCC G[GA]^CG[CT]C KasI 1164 GGCGCC G^GCGCC MaeI 992 CTAG C^TAG MluNI 1014 TGGCCA TGG^CCA MscI 1014 TGGCCA TGG^CCA MvnI 157 CGCG CG^CG NarI 1164 GGCGCC GG^CGCC PaeR7I 576 CTCGAG C^TCGAG PflFI 445 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1222 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 891 GACTC GAGTC(4/5) RcaI 1382 TCATGA T^CATGA SbfI 871 CCTGCAGG CCTGCA^GG ScaI 664 AGTACT AGT^ACT SfoI 1164 GGCGCC GGC^GCC SmlI 576 CTCGAG C^T[CT][GA]AG Sse8387I 871 CCTGCAGG CCTGCA^GG StyI 765 CCAAGG C^C[AT][AT]GG TfiI 370 GATTC G^A[AT]TC Tth111I 445 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1222 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 576 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AfaI 364 GTAC GT^AC 665 GTAC AspHI 198 GTGCAC G[AT]GC[AT]^C 624 GAGCAC BsiHKAI 198 GTGCAC G[AT]GC[AT]^C 624 GAGCAC BstAPI 200 GCACCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 733 GCATTCCCTGC BstYI 378 GGATCC [GA]^GATC[CT] 1364 GGATCT DsaI 182 CCGTGG C^C[GA][CT]GG 303 CCGTGG HinfI 370 GATTC G^A[ACGT]TC 891 GACTC MaeII 170 ACGT A^CGT 947 ACGT MaeIII 163 GTCAC ^GT[ACGT]AC 1039 GTCAC MflI 378 GGATCC [GA]^GATC[CT] 1364 GGATCT MslI 165 CACCTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 486 CACCCCTGTG NlaIII 261 CATG CATG^ 1383 CATG PpuMI 963 GGGACCC [GA]G^G[AT]CC[CT] 1019 AGGTCCC PvuII 836 CAGCTG CAG^CTG 1358 CAGCTG RsaI 364 GTAC GT^AC 665 GTAC SapI 809 GCTCTTC GCTCTTC(1/4) 1301 GCTCTTC SfaNI 346 GATGC GCATC(5/9) 787 GATGC TaqI 143 TCGA T^CGA 577 TCGA Tsp45I 163 GTCAC ^GT[GC]AC 1039 GTCAC TthHB8I 143 TCGA T^CGA 577 TCGA XhoII 378 GGATCC [GA]^GATC[CT] 1364 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AciI AluI AlwI ApaI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI Ksp632I MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIV PstI Sau3AI Sau96I ScrFI SfcI SmaI TseI TspRI XmaCI XmaI