Restriction map for
pF1KB5706
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# NO CUT ENZYMES ( 134 enzymes) #
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AatII Acc65I AccII AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BciVI BcnI BfrI BglI
BglII BlnI BsaAI BsaHI BsaI
BsgI BsiEI BsiWI BspDI BspLU11I
BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI BstXI Bsu36I CfoI Cfr10I
ClaI CpoI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MluI
MluNI MscI MvnI NaeI NarI
NciI NcoI NgoMIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I StuI
SwaI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 1499 GTATAC GT^[AC][GT]AC
AccIII 1674 TCCGGA T^CCGGA
AflIII 651 ACGTGT A^C[GA][CT]GT
Asp700I 1069 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 2126 GAGCAC G[AT]GC[AT]^C
AvaI 1388 CCCGAG C^[CT]CG[GA]G
BanI 708 GGTGCC G^G[CT][GA]CC
BbsI 153 GAAGAC GAAGAC(2/6)
BclI 539 TGATCA T^GATCA
BlpI 1654 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1654 GCTAAGC GC^T[ACGT]AGC
BpuAI 153 GAAGAC GAAGAC(2/6)
BsaBI 1594 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1674 TCCGGA [AT]^CCGG[AT]
BseAI 1674 TCCGGA T^CCGGA
BsiHKAI 2126 GAGCAC G[AT]GC[AT]^C
BsmBI 8 GAGACG CGTCTC(1/5)
BsoBI 1388 CCCGAG C^[CT]CG[GA]G
BspEI 1674 TCCGGA T^CCGGA
BspHI 2112 TCATGA T^CATGA
Bst1107I 1499 GTATAC GTA^TAC
BstYI 469 GGATCT [GA]^GATC[CT]
BstZ17I 1499 GTATAC GTA^TAC
CelII 1654 GCTAAGC GC^T[ACGT]AGC
DraI 1192 TTTAAA TTT^AAA
FauI 1220 CCCGC CCCGC(4/6)
FbaI 539 TGATCA T^GATCA
HapII 1675 CCGG C^CGG
HpaII 1675 CCGG C^CGG
MaeII 651 ACGT A^CGT
MamI 1594 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1103 CAATTG C^AATTG
MflI 469 GGATCT [GA]^GATC[CT]
MroI 1674 TCCGGA T^CCGGA
MspI 1675 CCGG C^CGG
MunI 1103 CAATTG C^AATTG
PleI 1053 GAGTC GAGTC(4/5)
PpuMI 1890 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 2112 TCATGA T^CATGA
ScaI 2034 AGTACT AGT^ACT
SexAI 1815 ACCTGGT A^CC[AT]GGT
SpeI 1569 ACTAGT A^CTAGT
TaqI 117 TCGA T^CGA
TthHB8I 117 TCGA T^CGA
XhoII 469 GGATCT [GA]^GATC[CT]
XmnI 1069 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AcsI 1147 AAATTT [GA]^AATT[CT]
1922 AAATTT
AlwNI 195 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
844 CAGTGCCTG
ApaI 1454 GGGCCC GGGCC^C
1985 GGGCCC
ApoI 1147 AAATTT [GA]^AATT[CT]
1922 AAATTT
BanII 1454 GGGCCC G[GA]GC[CT]^C
1985 GGGCCC
Bsp1407I 209 TGTACA T^GTACA
1899 TGTACA
BsrGI 209 TGTACA T^GTACA
1899 TGTACA
BstF5I 1641 GGATG GGATG(2/0)
1908 GGATG
DsaI 17 CCACGG C^C[GA][CT]GG
2018 CCGTGG
EcoNI 273 CCTTATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1368 CCTCAATGAGG
FokI 1641 GGATG GGATG(9/13)
1908 GGATG
MaeIII 350 GTTAC ^GT[ACGT]AC
1237 GTTAC
MslI 1228 CATGTCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1366 CACCTCAATG
NheI 1659 GCTAGC G^CTAGC
1995 GCTAGC
PstI 1241 CTGCAG CTGCA^G
1841 CTGCAG
SspBI 209 TGTACA T^GTACA
1899 TGTACA
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BfaI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrI BssKI BstNI
Cac8I Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII EcoT14I Fnu4HI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MboI MboII MnlI MseI
MspA1I MvaI MwoI NdeI NdeII
NlaIII NlaIV PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TfiI Tru9I TseI
Tsp509I TspRI