Restriction map for
pF1KB7911
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# NO CUT ENZYMES ( 177 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BbvI BcgI BciVI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI Hin1I HindIII
HpaI KasI KpnI KspI MaeI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MseI MslI
MunI NaeI NarI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TthHB8I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 433 GTAGAC GT^[AC][GT]AC
AccII 115 CGCG CG^CG
AfaI 138 GTAC GT^AC
AluI 261 AGCT AG^CT
AlwI 50 GGATC GGATC(4/5)
AspHI 205 GTGCTC G[AT]GC[AT]^C
AspI 73 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 13 CCCGAG C^[CT]CG[GA]G
AvaII 18 GGACC G^G[AT]CC
BclI 41 TGATCA T^GATCA
BmyI 205 GTGCTC G[AGT]GC[ACT]^C
BsiHKAI 205 GTGCTC G[AT]GC[AT]^C
BsmI 192 GAATGC GAATGC(1/-1)
BsoBI 13 CCCGAG C^[CT]CG[GA]G
Bsp1286I 205 GTGCTC G[AGT]GC[ACT]^C
BspMI 26 ACCTGC ACCTGC(4/8)
BsrDI 166 GCAATG GCAATG(2/0)
BsrI 197 CCAGT ACTGG(1/-1)
BstNI 47 CCAGG CC^[AT]GG
BstUI 115 CGCG CG^CG
Cac8I 121 GCCAGC GC[ACGT]^[ACGT]GC
DraII 17 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 215 CCATGG C^C[GA][CT]GG
EcoO109I 17 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRII 47 CCAGG ^CC[AT]GG
FbaI 41 TGATCA T^GATCA
Fnu4HI 118 GCCGC GC^[ACGT]GC
HincII 79 GTCAAC GT[CT]^[GA]AC
HindII 79 GTCAAC GT[CT]^[GA]AC
HinfI 343 GACTC G^A[ACGT]TC
HphI 44 TCACC GGTGA(8/7)
ItaI 118 GCCGC GC^[ACGT]GC
MaeIII 244 GTTAC ^GT[ACGT]AC
MspA1I 235 CAGCGG C[AC]G^C[GT]G
MvaI 47 CCAGG CC^[AT]GG
MvnI 115 CGCG CG^CG
NcoI 215 CCATGG C^CATGG
NlaIII 216 CATG CATG^
PflFI 73 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 343 GACTC GAGTC(4/5)
PpuMI 17 AGGACCT [GA]G^G[AT]CC[CT]
RsaI 138 GTAC GT^AC
SapI 349 GAAGAGC GCTCTTC(1/4)
StuI 302 AGGCCT AGG^CCT
TspRI 198 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 73 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 215 CCATGGAAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BcnI 69 CCCGG CC^[GC]GG
98 CCCGG
BstF5I 146 CATCC GGATG(2/0)
369 GGATG
DdeI 210 CTCAG C^T[ACGT]AG
306 CTCAG
DpnII 42 GATC ^GATC
51 GATC
EcoT14I 89 CCAAGG C^C[AT][AT]GG
215 CCATGG
FauI 113 CCCGC CCCGC(4/6)
279 GCGGG
FokI 146 CATCC GGATG(9/13)
369 GGATG
HaeII 63 GGCGCT [GA]GCGC^[CT]
103 AGCGCC
HapII 70 CCGG C^CGG
99 CCGG
HgaI 186 GACGC GACGC(5/10)
125 GCGTC
HpaII 70 CCGG C^CGG
99 CCGG
MboI 42 GATC ^GATC
51 GATC
MspI 70 CCGG C^CGG
99 CCGG
NciI 69 CCCGG CC^[GC]GG
98 CCCGG
NdeII 42 GATC ^GATC
51 GATC
Sau3AI 42 GATC ^GATC
51 GATC
StyI 89 CCAAGG C^C[AT][AT]GG
215 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AciI BbsI BpuAI BsaJI BssKI
CfoI Cfr13I EarI HaeIII HhaI
HinP1I Ksp632I MboII MnlI MwoI
Sau96I ScrFI