Restriction map for
pF1KB7904
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AvaI AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspEI BsrBI BsrFI BsrGI
BssHII BssKI BssSI Bst1107I BstAPI
BstBI BstNI BstUI BstZ17I Bsu36I
CelII Cfr10I CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRII
EcoRV EcoT22I FbaI FseI HaeII
HapII Hin1I HpaI HpaII KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MspI MunI
MvaI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PvuI RsrII SacI
SacII SbfI ScaI ScrFI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tsp45I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 596 GTCGAC GT^[AC][GT]AC
AclI 18 AACGTT AA^CGTT
AfaI 196 GTAC GT^AC
AflIII 332 ACATGT A^C[GA][CT]GT
AlwI 732 GGATC GGATC(4/5)
AseI 211 ATTAAT AT^TAAT
AsnI 211 ATTAAT AT^TAAT
Asp700I 274 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 834 GAGCAC G[AT]GC[AT]^C
AvaII 777 GGACC G^G[AT]CC
AviII 293 TGCGCA TGC^GCA
BglII 276 AGATCT A^GATCT
BmyI 834 GAGCAC G[AGT]GC[ACT]^C
BsaJI 767 CCAAGG C^C[ACGT][ACGT]GG
BsiHKAI 834 GAGCAC G[AT]GC[AT]^C
BsmFI 776 GGGAC GGGAC(10/14)
Bsp1286I 834 GAGCAC G[AGT]GC[ACT]^C
BspDI 373 ATCGAT AT^CGAT
BspHI 165 TCATGA T^CATGA
BspLU11I 332 ACATGT A^CATGT
BspMI 131 GCAGGT ACCTGC(4/8)
BsrI 638 ACTGG ACTGG(1/-1)
BstEII 762 GGTAACC G^GT[ACGT]ACC
BstPI 762 GGTAACC G^GT[ACGT]ACC
BstXI 915 CCACAGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 294 GCGC GCG^C
ClaI 373 ATCGAT AT^CGAT
DraII 776 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoNI 950 CCTACCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 776 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 762 GGTAACC G^GT[ACGT]ACC
EcoRI 583 GAATTC G^AATTC
EcoT14I 767 CCAAGG C^C[AT][AT]GG
FauI 249 GCGGG CCCGC(4/6)
FspI 293 TGCGCA TGC^GCA
HgaI 905 GACGC GACGC(5/10)
HhaI 294 GCGC GCG^C
HinP1I 294 GCGC G^CGC
HincII 596 GTCGAC GT[CT]^[GA]AC
HindII 596 GTCGAC GT[CT]^[GA]AC
HindIII 620 AAGCTT A^AGCTT
HphI 145 GGTGA GGTGA(8/7)
MaeII 19 ACGT A^CGT
MslI 862 CACAGCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 244 CAGCTG C[AC]G^C[GT]G
NspI 332 ACATGT [GA]CATG^[CT]
PacI 208 TTAATTAA TTAAT^TAA
PpuMI 776 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 211 ATTAAT AT^TAAT
Psp1406I 18 AACGTT AA^CGTT
PvuII 244 CAGCTG CAG^CTG
RcaI 165 TCATGA T^CATGA
RsaI 196 GTAC GT^AC
SalI 596 GTCGAC G^TCGAC
SapI 739 GCTCTTC GCTCTTC(1/4)
StuI 157 AGGCCT AGG^CCT
StyI 767 CCAAGG C^C[AT][AT]GG
XmnI 274 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 954 CCGC CCGC(-3/-1)
249 GCGG
AcsI 583 GAATTC [GA]^AATT[CT]
628 AAATTT
ApoI 583 GAATTC [GA]^AATT[CT]
628 AAATTT
BbsI 317 GAAGAC GAAGAC(2/6)
394 GAAGAC
BfaI 152 CTAG C^TAG
976 CTAG
BpuAI 317 GAAGAC GAAGAC(2/6)
394 GAAGAC
BsiYI 767 CCAAGGCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
950 CCTACCGCAGG
BslI 767 CCAAGGCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
950 CCTACCGCAGG
BsmAI 344 GAGAC GTCTC(1/5)
635 GAGAC
BsrDI 487 GCAATG GCAATG(2/0)
682 GCAATG
BstYI 276 AGATCT [GA]^GATC[CT]
732 GGATCT
Cac8I 225 GCAAGC GC[ACGT]^[ACGT]GC
979 GCCTGC
Cfr13I 777 GGACC G^G[ACGT]CC
925 GGCCC
EarI 161 CTCTTC CTCTTC(1/4)
740 CTCTTC
Ksp632I 161 CTCTTC CTCTTC(1/4)
740 CTCTTC
MaeI 152 CTAG C^TAG
976 CTAG
MaeIII 219 GTTAC ^GT[ACGT]AC
763 GTAAC
MflI 276 AGATCT [GA]^GATC[CT]
732 GGATCT
NlaIV 776 GGGACC GG[ACGT]^[ACGT]CC
777 GGACCC
PstI 648 CTGCAG CTGCA^G
839 CTGCAG
Sau96I 777 GGACC G^G[ACGT]CC
925 GGCCC
TspRI 113 CACTG CA[GC]TG[ACGT][ACGT]^
837 CACTG
XhoII 276 AGATCT [GA]^GATC[CT]
732 GGATCT
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AluI BbvI BstF5I DdeI DpnII
Fnu4HI FokI HaeIII HinfI ItaI
MboI MboII MnlI MseI MwoI
NdeII NlaIII Sau3AI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp509I
TthHB8I