Restriction map for
pF1KB6274
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# NO CUT ENZYMES ( 104 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AocI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BcgI
BciVI BclI BfrI BlnI BsaBI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI DraI
DraIII Eam1105I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FspI HincII HindII HindIII HpaI
KpnI MamI MfeI MluI MroI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
SalI SapI SbfI ScaI SexAI
SfiI SfuI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AcsI 235 GAATTT [GA]^AATT[CT]
AflIII 1200 ACGTGT A^C[GA][CT]GT
ApoI 235 GAATTT [GA]^AATT[CT]
Asp700I 1512 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI 1468 GCTGAGG CCTCAGC(-5/-2)
BglII 1139 AGATCT A^GATCT
BlpI 717 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 717 GCTCAGC GC^T[ACGT]AGC
BsaWI 743 ACCGGA [AT]^CCGG[AT]
BsiEI 1233 CGGCCG CG[GA][CT]^CG
BsmBI 786 CGTCTC CGTCTC(1/5)
BstXI 1587 CCAGCCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 717 GCTCAGC GC^T[ACGT]AGC
CpoI 1280 CGGACCG CG^G[AT]CCG
EagI 1233 CGGCCG C^GGCCG
EclXI 1233 CGGCCG C^GGCCG
Eco52I 1233 CGGCCG C^GGCCG
FseI 1234 GGCCGGCC GGCCGG^CC
KspI 471 CCGCGG CCGC^GG
MseI 1355 TTAA T^TAA
NspI 1499 GCATGT [GA]CATG^[CT]
PflMI 256 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1023 GAGTC GAGTC(4/5)
RsrII 1280 CGGACCG CG^G[AT]CCG
SacI 1123 GAGCTC GAGCT^C
SacII 471 CCGCGG CCGC^GG
SgrAI 415 CACCGGCG C[GA]^CCGG[CT]G
SmlI 799 CTCAAG C^T[CT][GA]AG
TfiI 28 GATTC G^A[AT]TC
Tru9I 1355 TTAA T^TAA
Van91I 256 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 519 CCACGTAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1512 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Aor51HI 1004 AGCGCT AGC^GCT
1956 AGCGCT
AvaI 1522 CCCGGG C^[CT]CG[GA]G
1736 CCCGGG
BalI 1748 TGGCCA TGG^CCA
1985 TGGCCA
BbsI 630 GAAGAC GAAGAC(2/6)
1516 GTCTTC
BglI 1901 GCCCGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1910 GCCGCAGCGGC
BpuAI 630 GAAGAC GAAGAC(2/6)
1516 GTCTTC
BsaAI 520 CACGTA [CT]AC^GT[GA]
2023 TACGTG
BsaI 673 GAGACC GGTCTC(1/5)
568 GGTCTC
BsoBI 1522 CCCGGG C^[CT]CG[GA]G
1736 CCCGGG
BsrDI 1578 CATTGC GCAATG(2/0)
192 GCAATG
DrdI 1153 GACGTGATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1972 GACCGCCTAGTC
EarI 957 CTCTTC CTCTTC(1/4)
1402 CTCTTC
Eco47III 1004 AGCGCT AGC^GCT
1956 AGCGCT
HgaI 1031 GACGC GACGC(5/10)
785 GCGTC
HinfI 28 GATTC G^A[ACGT]TC
1023 GAGTC
Ksp632I 957 CTCTTC CTCTTC(1/4)
1402 CTCTTC
MluNI 1748 TGGCCA TGG^CCA
1985 TGGCCA
MscI 1748 TGGCCA TGG^CCA
1985 TGGCCA
NaeI 713 GCCGGC GCC^GGC
1235 GCCGGC
NgoMIV 713 GCCGGC G^CCGGC
1235 GCCGGC
PvuII 484 CAGCTG CAG^CTG
1342 CAGCTG
SmaI 1522 CCCGGG CCC^GGG
1736 CCCGGG
XmaCI 1522 CCCGGG C^CCGGG
1736 CCCGGG
XmaI 1522 CCCGGG C^CCGGG
1736 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaI ApaLI
AspHI AvaII BanI BanII BbeI
BbvI BcnI BfaI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrBI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HpaII
HphI ItaI KasI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII