Restriction map for
pF1KB6246
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# NO CUT ENZYMES ( 106 enzymes) #
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Acc65I AccI AclI AcsI AflII
AflIII AgeI AhdI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BbrPI
BcgI BciVI BclI BfaI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsiWI BsmI Bsp1407I BspDI BspLU11I
BsrFI BsrGI BssHII Bst1107I BstBI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraI Eam1105I Eco47III EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MaeI MamI MfeI MluI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspBI SspI SwaI TaqI
Tsp509I TthHB8I Van91I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 1393 GACGTC GACGT^C
AccII 1579 CGCG CG^CG
AccIII 1992 TCCGGA T^CCGGA
Alw44I 1225 GTGCAC G^TGCAC
AocI 242 CCTCAGG CC^T[ACGT]AGG
ApaLI 1225 GTGCAC G^TGCAC
AspI 727 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1963 GGCGCC GGCGC^C
BglII 1475 AGATCT A^GATCT
BseAI 1992 TCCGGA T^CCGGA
BsmBI 1191 CGTCTC CGTCTC(1/5)
BspEI 1992 TCCGGA T^CCGGA
BspHI 1371 TCATGA T^CATGA
BsrDI 155 GCAATG GCAATG(2/0)
BstEII 1185 GGTGACC G^GT[ACGT]ACC
BstPI 1185 GGTGACC G^GT[ACGT]ACC
BstUI 1579 CGCG CG^CG
Bsu36I 242 CCTCAGG CC^T[ACGT]AGG
DraIII 1897 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 24 GACAATCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 873 CTCTTC CTCTTC(1/4)
Eco81I 242 CCTCAGG CC^T[ACGT]AGG
EcoNI 2007 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1185 GGTGACC G^GT[ACGT]ACC
HgaI 731 GCGTC GACGC(5/10)
KasI 1963 GGCGCC G^GCGCC
Ksp632I 873 CTCTTC CTCTTC(1/4)
MroI 1992 TCCGGA T^CCGGA
MseI 1709 TTAA T^TAA
MvnI 1579 CGCG CG^CG
NarI 1963 GGCGCC GG^CGCC
PflFI 727 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2003 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 1371 TCATGA T^CATGA
SacI 252 GAGCTC GAGCT^C
SapI 872 GCTCTTC GCTCTTC(1/4)
ScaI 431 AGTACT AGT^ACT
SexAI 1898 ACCAGGT A^CC[AT]GGT
SfoI 1963 GGCGCC GGC^GCC
SphI 1769 GCATGC GCATG^C
Tru9I 1709 TTAA T^TAA
Tth111I 727 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcyI 1393 GACGTC G[GA]^CG[CT]C
1963 GGCGCC
AlwI 360 GATCC GGATC(4/5)
1059 GATCC
ApaI 634 GGGCCC GGGCC^C
1305 GGGCCC
BalI 314 TGGCCA TGG^CCA
931 TGGCCA
BglI 1093 GCCATGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1726 GCCGCCCTGGC
BsaHI 1393 GACGTC G[GA]^CG[CT]C
1963 GGCGCC
BsaWI 1007 ACCGGA [AT]^CCGG[AT]
1992 TCCGGA
BsiEI 764 CGGCCG CG[GA][CT]^CG
1241 CGGCCG
BssSI 880 CACGAG CACGAG(-5/-1)
1222 CTCGTG
DsaI 140 CCACGG C^C[GA][CT]GG
1832 CCGTGG
EagI 764 CGGCCG C^GGCCG
1241 CGGCCG
EclXI 764 CGGCCG C^GGCCG
1241 CGGCCG
Eco52I 764 CGGCCG C^GGCCG
1241 CGGCCG
HaeII 1963 GGCGCC [GA]GCGC^[CT]
1978 AGCGCC
Hin1I 1393 GACGTC G[GA]^CG[CT]C
1963 GGCGCC
MluNI 314 TGGCCA TGG^CCA
931 TGGCCA
MscI 314 TGGCCA TGG^CCA
931 TGGCCA
MslI 1491 CACCAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1665 CACCCTGGTG
NspI 1112 ACATGC [GA]CATG^[CT]
1769 GCATGC
PleI 1204 GACTC GAGTC(4/5)
168 GAGTC
SfiI 1092 GGCCATGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1725 GGCCGCCCTGGCC
SmaI 709 CCCGGG CCC^GGG
1948 CCCGGG
TfiI 10 GAATC G^A[AT]TC
1368 GAATC
XcmI 477 CCAGCAGCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
503 CCAAAGAGGCACTGG
XmaCI 709 CCCGGG C^CCGGG
1948 CCCGGG
XmaI 709 CCCGGG C^CCGGG
1948 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BcnI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TseI Tsp45I TspRI XhoII