################################## # NO CUT ENZYMES ( 116 enzymes) # ################################## Acc65I AccII AccIII AclI AflII AgeI Alw44I AocI ApaI ApaLI AscI Asp700I Asp718I AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BclI BcnI BfrI BglI BglII BlnI BsaAI BsaWI BseAI BseRI BsiEI BsiWI BsmI BspDI BspEI BspHI BsrDI BsrFI BssHII Bst1107I BstEII BstPI BstUI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraII DrdI EaeI EagI EclXI Eco52I Eco81I EcoO109I EcoO65I FbaI FseI FspI HapII HindIII HpaI HpaII KasI KpnI KspI MflI MluI MluNI MroI MscI MspI MvnI NaeI NarI NciI NdeI NgoMIV NotI NruI PacI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SapI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I StuI XbaI XcmI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AatII 1336 GACGTC GACGT^C AhdI 508 GACGGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 1875 GATCC GGATC(4/5) Aor51HI 2218 AGCGCT AGC^GCT AseI 681 ATTAAT AT^TAAT AsnI 681 ATTAAT AT^TAAT AspEI 508 GACGGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 651 GGACC G^G[AT]CC BciVI 228 GTATCC GTATCC(6/5) BlpI 2094 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2094 GCTGAGC GC^T[ACGT]AGC BsaBI 1323 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 318 GGTCTC GGTCTC(1/5) BsmBI 490 GAGACG CGTCTC(1/5) BsmFI 1829 GGGAC GGGAC(10/14) BspLU11I 150 ACATGT A^CATGT BsrBI 161 CCGCTC CCGCTC(-3/-3) BssSI 1526 CTCGTG CACGAG(-5/-1) BstBI 1376 TTCGAA TT^CGAA CelII 2094 GCTGAGC GC^T[ACGT]AGC DraI 1977 TTTAAA TTT^AAA DraIII 436 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 508 GACGGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2218 AGCGCT AGC^GCT EcoNI 1512 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 426 GAATTC G^AATTC EcoRV 1045 GATATC GAT^ATC EcoT22I 974 ATGCAT ATGCA^T FauI 160 CCCGC CCCGC(4/6) MamI 1323 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 473 CAATTG C^AATTG MslI 748 CACTACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 1460 CCGCTG C[AC]G^C[GT]G MunI 473 CAATTG C^AATTG NcoI 1296 CCATGG C^CATGG NheI 1852 GCTAGC G^CTAGC NsiI 974 ATGCAT ATGCA^T NspV 1376 TTCGAA TT^CGAA PshBI 681 ATTAAT AT^TAAT ScaI 2194 AGTACT AGT^ACT SfuI 1376 TTCGAA TT^CGAA SphI 2120 GCATGC GCATG^C SwaI 1976 ATTTAAAT ATTT^AAAT ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcsI 426 GAATTC [GA]^AATT[CT] 1380 AAATTT AcyI 361 GGCGTC G[GA]^CG[CT]C 1336 GACGTC AflIII 150 ACATGT A^C[GA][CT]GT 1496 ACACGT ApoI 426 GAATTC [GA]^AATT[CT] 1380 AAATTT BbsI 799 GAAGAC GAAGAC(2/6) 1757 GAAGAC BpuAI 799 GAAGAC GAAGAC(2/6) 1757 GAAGAC BsaHI 361 GGCGTC G[GA]^CG[CT]C 1336 GACGTC Bsp1407I 251 TGTACA T^GTACA 611 TGTACA BsrGI 251 TGTACA T^GTACA 611 TGTACA BstAPI 1522 GCAGCTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1785 GCAGTTTCTGC CfoI 1152 GCGC GCG^C 2219 GCGC DsaI 314 CCGTGG C^C[GA][CT]GG 1296 CCATGG EcoT14I 1296 CCATGG C^C[AT][AT]GG 1451 CCAAGG HaeII 1151 GGCGCT [GA]GCGC^[CT] 2218 AGCGCT HaeIII 459 GGCC GG^CC 1515 GGCC HgaI 492 GACGC GACGC(5/10) 362 GCGTC HhaI 1152 GCGC GCG^C 2219 GCGC Hin1I 361 GGCGTC G[GA]^CG[CT]C 1336 GACGTC HinP1I 1152 GCGC G^CGC 2219 GCGC PaeR7I 980 CTCGAG C^TCGAG 2134 CTCGAG PstI 1057 CTGCAG CTGCA^G 2240 CTGCAG SalI 1223 GTCGAC G^TCGAC 1585 GTCGAC SspBI 251 TGTACA T^GTACA 611 TGTACA SspI 1089 AATATT AAT^ATT 1426 AATATT StyI 1296 CCATGG C^C[AT][AT]GG 1451 CCAAGG XhoI 980 CTCGAG C^TCGAG 2134 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AccI AciI AfaI AluI AlwNI AspHI AspI AvaI BbvI BfaI BmyI BpmI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV NspI PflFI PflMI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I