Restriction map for pF1KB6235
##################################
# NO CUT ENZYMES (  116 enzymes) #
##################################

Acc65I      AccII       AccIII      AclI        AflII       
AgeI        Alw44I      AocI        ApaI        ApaLI       
AscI        Asp700I     Asp718I     AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbvCI       BcgI        BclI        BcnI        
BfrI        BglI        BglII       BlnI        BsaAI       
BsaWI       BseAI       BseRI       BsiEI       BsiWI       
BsmI        BspDI       BspEI       BspHI       BsrDI       
BsrFI       BssHII      Bst1107I    BstEII      BstPI       
BstUI       BstYI       BstZ17I     Bsu36I      Cfr10I      
ClaI        CpoI        DraII       DrdI        EaeI        
EagI        EclXI       Eco52I      Eco81I      EcoO109I    
EcoO65I     FbaI        FseI        FspI        HapII       
HindIII     HpaI        HpaII       KasI        KpnI        
KspI        MflI        MluI        MluNI       MroI        
MscI        MspI        MvnI        NaeI        NarI        
NciI        NdeI        NgoMIV      NotI        NruI        
PacI        PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SapI        
SbfI        SexAI       SfiI        SfoI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    StuI        
XbaI        XcmI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AatII        1336  GACGTC               GACGT^C
AhdI          508  GACGGTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI         1875  GATCC                GGATC(4/5)
Aor51HI      2218  AGCGCT               AGC^GCT
AseI          681  ATTAAT               AT^TAAT
AsnI          681  ATTAAT               AT^TAAT
AspEI         508  GACGGTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII         651  GGACC                G^G[AT]CC
BciVI         228  GTATCC               GTATCC(6/5)
BlpI         2094  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2094  GCTGAGC              GC^T[ACGT]AGC
BsaBI        1323  GATTCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          318  GGTCTC               GGTCTC(1/5)
BsmBI         490  GAGACG               CGTCTC(1/5)
BsmFI        1829  GGGAC                GGGAC(10/14)
BspLU11I      150  ACATGT               A^CATGT
BsrBI         161  CCGCTC               CCGCTC(-3/-3)
BssSI        1526  CTCGTG               CACGAG(-5/-1)
BstBI        1376  TTCGAA               TT^CGAA
CelII        2094  GCTGAGC              GC^T[ACGT]AGC
DraI         1977  TTTAAA               TTT^AAA
DraIII        436  CACTCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I      508  GACGGTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     2218  AGCGCT               AGC^GCT
EcoNI        1512  CCTGGCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         426  GAATTC               G^AATTC
EcoRV        1045  GATATC               GAT^ATC
EcoT22I       974  ATGCAT               ATGCA^T
FauI          160  CCCGC                CCCGC(4/6)
MamI         1323  GATTCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          473  CAATTG               C^AATTG
MslI          748  CACTACTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       1460  CCGCTG               C[AC]G^C[GT]G
MunI          473  CAATTG               C^AATTG
NcoI         1296  CCATGG               C^CATGG
NheI         1852  GCTAGC               G^CTAGC
NsiI          974  ATGCAT               ATGCA^T
NspV         1376  TTCGAA               TT^CGAA
PshBI         681  ATTAAT               AT^TAAT
ScaI         2194  AGTACT               AGT^ACT
SfuI         1376  TTCGAA               TT^CGAA
SphI         2120  GCATGC               GCATG^C
SwaI         1976  ATTTAAAT             ATTT^AAAT


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcsI          426  GAATTC               [GA]^AATT[CT]
             1380  AAATTT              
AcyI          361  GGCGTC               G[GA]^CG[CT]C
             1336  GACGTC              
AflIII        150  ACATGT               A^C[GA][CT]GT
             1496  ACACGT              
ApoI          426  GAATTC               [GA]^AATT[CT]
             1380  AAATTT              
BbsI          799  GAAGAC               GAAGAC(2/6)
             1757  GAAGAC              
BpuAI         799  GAAGAC               GAAGAC(2/6)
             1757  GAAGAC              
BsaHI         361  GGCGTC               G[GA]^CG[CT]C
             1336  GACGTC              
Bsp1407I      251  TGTACA               T^GTACA
              611  TGTACA              
BsrGI         251  TGTACA               T^GTACA
              611  TGTACA              
BstAPI       1522  GCAGCTCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1785  GCAGTTTCTGC         
CfoI         1152  GCGC                 GCG^C
             2219  GCGC                
DsaI          314  CCGTGG               C^C[GA][CT]GG
             1296  CCATGG              
EcoT14I      1296  CCATGG               C^C[AT][AT]GG
             1451  CCAAGG              
HaeII        1151  GGCGCT               [GA]GCGC^[CT]
             2218  AGCGCT              
HaeIII        459  GGCC                 GG^CC
             1515  GGCC                
HgaI          492  GACGC                GACGC(5/10)
              362  GCGTC               
HhaI         1152  GCGC                 GCG^C
             2219  GCGC                
Hin1I         361  GGCGTC               G[GA]^CG[CT]C
             1336  GACGTC              
HinP1I       1152  GCGC                 G^CGC
             2219  GCGC                
PaeR7I        980  CTCGAG               C^TCGAG
             2134  CTCGAG              
PstI         1057  CTGCAG               CTGCA^G
             2240  CTGCAG              
SalI         1223  GTCGAC               G^TCGAC
             1585  GTCGAC              
SspBI         251  TGTACA               T^GTACA
              611  TGTACA              
SspI         1089  AATATT               AAT^ATT
             1426  AATATT              
StyI         1296  CCATGG               C^C[AT][AT]GG
             1451  CCAAGG              
XhoI          980  CTCGAG               C^TCGAG
             2134  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   73 enzymes) #
#########################################

AccI        AciI        AfaI        AluI        AlwNI       
AspHI       AspI        AvaI        BbvI        BfaI        
BmyI        BpmI        BsaJI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsoBI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstXI       Cac8I       Cfr13I      DdeI        DpnII       
EarI        EcoRII      Fnu4HI      FokI        HincII      
HindII      HinfI       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       NspI        PflFI       PflMI       
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
Tth111I     TthHB8I     Van91I      
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