Restriction map for
pF1KB6229
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# NO CUT ENZYMES ( 98 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI AocI
ApoI AscI AseI AsnI AspEI
AspI AvrII BamHI BciVI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BseAI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII Eam1105I Eco81I
EcoNI EcoO65I EcoRI EcoRV FseI
HindIII HpaI KspI MamI MfeI
MluI MroI MseI MunI NaeI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SexAI SgrAI SnaBI SpeI SspBI
SspI SwaI Tru9I Tsp509I Tth111I
Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 452 GGTACC G^GTACC
AccII 1536 CGCG CG^CG
AcyI 1876 GGCGCC G[GA]^CG[CT]C
Asp718I 452 GGTACC G^GTACC
AviII 1544 TGCGCA TGC^GCA
BalI 936 TGGCCA TGG^CCA
BbeI 1876 GGCGCC GGCGC^C
BbrPI 1069 CACGTG CAC^GTG
BcgI 1402 GCACAGTTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 1069 CACGTG [CT]AC^GT[GA]
BsaHI 1876 GGCGCC G[GA]^CG[CT]C
BsaI 367 GAGACC GGTCTC(1/5)
BsaWI 143 ACCGGA [AT]^CCGG[AT]
BsiEI 314 CGGCCG CG[GA][CT]^CG
BsiWI 1292 CGTACG C^GTACG
BsmBI 1768 GAGACG CGTCTC(1/5)
BsrDI 1727 GCAATG GCAATG(2/0)
BstBI 1410 TTCGAA TT^CGAA
BstUI 1536 CGCG CG^CG
DrdI 1459 GACATGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 314 CGGCCG C^GGCCG
EclXI 314 CGGCCG C^GGCCG
Eco52I 314 CGGCCG C^GGCCG
EcoT22I 257 ATGCAT ATGCA^T
FspI 1544 TGCGCA TGC^GCA
Hin1I 1876 GGCGCC G[GA]^CG[CT]C
HincII 200 GTCAAC GT[CT]^[GA]AC
HindII 200 GTCAAC GT[CT]^[GA]AC
KasI 1876 GGCGCC G^GCGCC
KpnI 452 GGTACC GGTAC^C
MaeIII 1054 GTGAC ^GT[ACGT]AC
MluNI 936 TGGCCA TGG^CCA
MscI 936 TGGCCA TGG^CCA
MvnI 1536 CGCG CG^CG
NarI 1876 GGCGCC GG^CGCC
NsiI 257 ATGCAT ATGCA^T
NspI 1040 GCATGC [GA]CATG^[CT]
NspV 1410 TTCGAA TT^CGAA
PmaCI 1069 CACGTG CAC^GTG
PmlI 1069 CACGTG CAC^GTG
SacI 1303 GAGCTC GAGCT^C
ScaI 1910 AGTACT AGT^ACT
SfiI 402 GGCCACCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1876 GGCGCC GGC^GCC
SfuI 1410 TTCGAA TT^CGAA
SmaI 1832 CCCGGG CCC^GGG
SphI 1040 GCATGC GCATG^C
StuI 377 AGGCCT AGG^CCT
TfiI 246 GATTC G^A[AT]TC
Tsp45I 1054 GTGAC ^GT[GC]AC
XmaCI 1832 CCCGGG C^CCGGG
XmaI 1832 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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AlwNI 244 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
349 CAGTGCCTG
Aor51HI 1490 AGCGCT AGC^GCT
1826 AGCGCT
ApaI 532 GGGCCC GGGCC^C
667 GGGCCC
BbvCI 715 GCTGAGG CCTCAGC(-5/-2)
928 GCTGAGG
BclI 1389 TGATCA T^GATCA
1850 TGATCA
BfaI 41 CTAG C^TAG
875 CTAG
BglI 403 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
505 GCCAGTGTGGC
BsmI 1343 GAATGC GAATGC(1/-1)
480 GCATTC
BstAPI 1559 GCAGTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1895 GCACCATCTGC
Eco47III 1490 AGCGCT AGC^GCT
1826 AGCGCT
FbaI 1389 TGATCA T^GATCA
1850 TGATCA
MaeI 41 CTAG C^TAG
875 CTAG
PpuMI 290 AGGACCT [GA]G^G[AT]CC[CT]
841 GGGACCC
SbfI 639 CCTGCAGG CCTGCA^GG
661 CCTGCAGG
Sse8387I 639 CCTGCAGG CCTGCA^GG
661 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AfaI AluI Alw44I AlwI
ApaLI Asp700I AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TseI TspRI TthHB8I
XcmI XhoII XmnI